REL
2022-09-16
2022-11-16
2022-11-16
Single particle cryo-EM of KdpFAB(Ser162-P, D307N)C in apo condition
0000-0002-9901-2065
Jan
Rheinberger
Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen
Nijenborgh 4
Groningen
Netherlands
9747 AG
0000-0001-7017-109X
Cristina
Paulino
Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen
Nijenborgh 4
Groningen
Netherlands
9747 AG
Silberberg JM
Stock C
Hielkema L
Corey RA
Rheinberger J
Wunnicke D
Dubach VRA
Stansfeld PJ
Hänelt I
Paulino C
2.5
10.6019/EMPIAR-11229
EMDB
molecule
EMD-14914
EMD-14915
EMD-14916
Silberberg JM
Stock C
Hielkema L
Corey RA
Rheinberger J
Wunnicke D
Dubach VRA
Stansfeld PJ
Hänelt I
Paulino C
Inhibited KdpFABC transitions into an E1 off-cycle state
eLife
Elife
11
2022
English
10.7554/elife.80988
36255052
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 1)
/data/raw_181029_KdpFABC-B-D307N-KCl-bCP75s3_ngc
micrographs - multiframe
TIFF
TIFF
787
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 2)
/data/raw_181031_KdpFABC-B-D307N-KCl-bCP75s5_ngc
micrographs - multiframe
TIFF
TIFF
1370
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 3)
/data/raw_181205_KdpFABC-B-D307N-KCl-bCP75s2_ngc
micrographs - multiframe
TIFF
TIFF
2213
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 4)
/data/raw_181214_KdpFABC-B-D307N-KCl-bCP75s6_ngc
micrographs - multiframe
TIFF
TIFF
1333
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 5)
/data/raw_190320_KdpFABC-B-D307N-KCl-bLH18s7_ngc
micrographs - multiframe
TIFF
TIFF
317
60
UNSIGNED BYTE
3838
1.012
3838
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 6)
/data/raw_190322_KdpFABC-B-D307N-KCl-bLH18s6_ngc
micrographs - multiframe
TIFF
TIFF
618
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 7)
/data/raw_190403_KdpFABC-B-D307N-KCl-bLH18s3_ngc
micrographs - multiframe
TIFF
TIFF
181
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 8)
/data/raw_190404_KdpFABC-B-D307N-KCl-bLH19s2_ngc
micrographs - multiframe
TIFF
TIFF
448
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 9)
/data/raw_190613_KdpFABC-B-D307N-KCl-bLH21s3_ngc
micrographs - multiframe
TIFF
TIFF
93
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 10)
/data/raw_190614_KdpFABC-B-D307N-KCl-bLH21s7_ngc
micrographs - multiframe
TIFF
TIFF
959
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 11)
/data/raw_190626_KdpFABC-B-D307N-KCl-bLH21s2_ngc
micrographs - multiframe
TIFF
TIFF
862
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 12)
/data/raw_190807_KdpFABC-B-D307N-KCl-bLH22s1_ngc
micrographs - multiframe
TIFF
TIFF
897
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 13)
/data/raw_190809_KdpFABC-B-D307N-KCl-bLH22s2_ngc
micrographs - multiframe
TIFF
TIFF
1248
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 14)
/data/raw_190814_KdpFABC-B-D307N-KCl-bLH23s1_ngc
micrographs - multiframe
TIFF
TIFF
584
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 15)
/data/raw_190816_KdpFABC-B-D307N-KCl-bLH23s2_ngc
micrographs - multiframe
TIFF
TIFF
954
60
UNSIGNED BYTE
3838
1.012
3710
1.012
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.