REL
2022-08-29
2022-11-14
2022-11-14
Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP
0000-0001-8261-4396
Denis
Chrétien
Institute of Genetics and Development of Rennes
263 avenue du Général Leclerc, Bâtiment 13
Rennes
France
35042
0000-0001-8261-4396
Denis
Chrétien
Institute of Genetics and Development of Rennes
263 avenue du Général Leclerc, Bâtiment 13
Rennes
France
35042
Guyomar C
Bousquet C
Ku S
Heumann J
Guilloux G
Gaillard N
Heichette C
Duchesne L
Steinmetz MO
Gibeaux R
Chrétien D
76.1
10.6019/EMPIAR-11264
EMDB
molecule
EMD-15740
Guyomar C
Bousquet C
Ku S
Heumann J
Guilloux G
Gaillard N
Heichette C
Duchesne L
Steinmetz MO
Gibeaux R
Chrétien D
Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts
bioRxiv
2021
English
10.1101/2021.07.14.452321
Single and dual-axis tilt series of microtubules assembled from purified tubulin in the presence of GMPCPP, decorated with kinesin motor domains, and vitrified by plunge freezing into liquid ethane
EMPIAR-11253
Single- and dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in the presence of GMPCPP and decorated with kinesin motor domains
/data/raw_tilt_series
tilt series
MRC
MRC
8
1
SIGNED 16 BIT INTEGER
variable
variable
variable
variable
Single- and dual-axis tilt series. The number of images in the tilt series is variable.
GMPCPP_S1_tomo1, GMPCPP_S1_tomo2, GMPCPP_S1_tomo3 and GMPCPP_S1_tomo4 are dual-axis tilt series, with a pixel spacing of 7.9 Å (image size 2048, 2048).
GMPCPP_S2_tomo1 and GMPCPP_S2_tomo2 are single-axis tilt series, with a pixel size of 3.7 Å (image size 4096,4096).
GMPCPP_tomoFig5 and GMPCPP_tomoFig6 are single-axis tilt series, with a pixel size of 2.1 Å (image size 4096,4096).