REL 2014-07-23 2014-08-07 2014-08-07 Alberto Bartesaghi Center for Cancer Research, National Cancer Institute Bethesda United States 20892 Sriram Subramaniam Center for Cancer Research, National Cancer Institute Bethesda United States 20892 Bartesaghi A Matthies D Banerjee S Merk A Subramaniam S 1 2015-07-01 REL

Directory structure reorganized. Entry has now full set of micrographs including frame alignment shifts, particle locations and defoci.

442.5 10.6019/EMPIAR-10013 EMDB EMD-5995 Full set of multi-frame micrographs /data/all micrographs - multiframe MRC MRC 509 38 32 BIT FLOAT 7420 0.6375 7676 0.6375

Full set of multiframe micrographs related to the dataset:10012. Related PDB: 3J7H. Please note that the number of frames varies between [5, 10, 25 and 38]! Although the image files are in MRC format, they have been compressed to save space. They can be uncompresssed using for example: for f in *.tbz; do tar -xjf $f -C mrc/ ; done Image files are numbered: BGal_000000_frames.tbz to BGal_000508_frames.tbz Frame alignment shifts in IMOD's transform format: BGal_000000.xf to BGal_000508.xf Picked particle locations in EMAN's boxer format: BGal_000000.box to BGal_000508.box Defocus per image in Angstroms: BGal_defocus.txt Additional details: Cs : 2.7 Anisotropic magnification (if known) ? : N/A frame rate : 2.5 to .33 frames/s dose per frame : 1.2 to 9 e-/A^2 alignment method/software : 10.1073/pnas.1402809111 which frame used for alignment reference: middle frame particle selection software : 10.1073/pnas.1402809111

Sample set of multi-frame micrographs /data/sample micrographs - multiframe DM4 DM4 5 38 1 38 32 BIT FLOAT 7420 0.6375 7676 0.6375

Selection of 5 multiframe micrographs related to the dataset:10012. Related PDB: 3J7H.