DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0004/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0004/defocus_estimation/series0004_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0004/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 43400.0, 65100.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 45.0 43000.0 44000.0 48000.0 1 42.0 52500.0 64000.0 63500.0 2 39.0 45000.0 47500.0 43000.0 3 36.0 62000.0 43500.0 60000.0 4 33.0 46000.0 62500.0 46000.0 5 30.0 47500.0 51000.0 49500.0 6 27.0 56500.0 59500.0 47000.0 7 24.0 55500.0 61000.0 43500.0 8 21.0 51000.0 60000.0 48500.0 9 18.0 52500.0 47000.0 50000.0 10 15.0 49000.0 64500.0 51500.0 11 12.0 61000.0 49500.0 43000.0 12 9.0 47500.0 47500.0 43000.0 13 6.0 51500.0 55500.0 58000.0 14 3.0 50500.0 43500.0 51500.0 15 0.1 51000.0 56500.0 51500.0 16 -0.1 65000.0 46000.0 65000.0 17 -3.0 50000.0 65000.0 45500.0 18 -6.0 48000.0 44500.0 65000.0 19 -9.0 44500.0 61500.0 65000.0 20 -12.0 48000.0 43000.0 64500.0 21 -15.0 46000.0 51000.0 63500.0 22 -18.0 57500.0 64500.0 57500.0 23 -21.0 47500.0 58500.0 54000.0 24 -24.0 63500.0 49000.0 52000.0 25 -27.0 64500.0 54500.0 64500.0 26 -30.0 48500.0 52500.0 50500.0 27 -33.0 62000.0 57500.0 43000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)