DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009)
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Input tilt-series file name:
/gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0002/defocus_estimation/stack_for_defocus_estimation.mrcs
Input tilt-angles file name:
/gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0002/defocus_estimation/series0002_tilts.txt
Output diagnostic file name:
/gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0002/defocus_estimation//diagnostic.mrc
Tile size:
384
Input CTF parameters:
CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]:
2.0, 200.0, 0.07, 10000.0, 1.5
Angstroms/Pixel: 1.50
Input Fitting parameters:
CTFMin[0,1], ResMin[A], ResMax[A]:
0.5, 100000.00, 6.0
dFMin[A], dFMax[A], FStep:
4.0, 6.00, 500.0
Summary of defocus gradients:
=============================
Image TiltAngle DF-left* DF-center* DF-right*
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0 -36.0 0.0 0.0 0.0
1 -33.0 0.0 0.0 0.0
2 -30.0 0.0 0.0 0.0
3 -27.0 0.0 0.0 0.0
4 -24.0 0.0 0.0 0.0
5 -21.0 0.0 0.0 0.0
6 -18.0 0.0 0.0 0.0
7 -15.0 0.0 0.0 0.0
8 -12.0 0.0 0.0 0.0
9 -9.0 0.0 0.0 0.0
10 -6.0 0.0 0.0 0.0
11 -3.0 0.0 0.0 0.0
12 -0.1 0.0 0.0 0.0
13 0.1 0.0 0.0 0.0
14 3.0 0.0 0.0 0.0
15 6.0 0.0 0.0 0.0
16 9.0 0.0 0.0 0.0
17 12.0 0.0 0.0 0.0
18 15.0 0.0 0.0 0.0
19 18.0 0.0 0.0 0.0
20 21.0 0.0 0.0 0.0
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* Approx. DF values corresponding to the X columns:
left center right
384 2112 3840
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Tilt angles are according to the convention used in TOMOCTF:
i.e., images with positive tilt angle have
increasing underfocus magnitude with the X axis (left to right)