DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0004/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0004/defocus_estimation/series0004_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0004/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.0, 200.0, 0.07, 10000.0, 1.5 Angstroms/Pixel: 1.50 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 6.0 dFMin[A], dFMax[A], FStep: 4.0, 6.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -36.0 0.0 0.0 0.0 1 -33.0 0.0 0.0 0.0 2 -30.0 0.0 0.0 0.0 3 -27.0 0.0 0.0 0.0 4 -24.0 0.0 0.0 0.0 5 -21.0 0.0 0.0 0.0 6 -18.0 0.0 0.0 0.0 7 -15.0 0.0 0.0 0.0 8 -12.0 0.0 0.0 0.0 9 -9.0 0.0 0.0 0.0 10 -6.0 0.0 0.0 0.0 11 -3.0 0.0 0.0 0.0 12 -0.1 0.0 0.0 0.0 13 0.1 0.0 0.0 0.0 14 3.0 0.0 0.0 0.0 15 6.0 0.0 0.0 0.0 16 9.0 0.0 0.0 0.0 17 12.0 0.0 0.0 0.0 18 15.0 0.0 0.0 0.0 19 18.0 0.0 0.0 0.0 20 21.0 0.0 0.0 0.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 2112 3840 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)