DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0003/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0003/defocus_estimation/series0003_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0003/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 42.0 58000.0 43000.0 51500.0 1 39.0 41000.0 45500.0 60000.0 2 36.0 57500.0 40000.0 42500.0 3 33.0 40000.0 57000.0 41000.0 4 30.0 45500.0 52000.0 54000.0 5 27.0 44500.0 60000.0 42000.0 6 24.0 45000.0 45500.0 58500.0 7 21.0 53500.0 60000.0 56000.0 8 18.0 47000.0 59500.0 60000.0 9 15.0 60000.0 55000.0 60000.0 10 12.0 52000.0 59000.0 60000.0 11 9.0 60000.0 60000.0 60000.0 12 6.0 60000.0 60000.0 60000.0 13 3.0 60000.0 60000.0 60000.0 14 -0.0 60000.0 60000.0 60000.0 15 -3.0 60000.0 60000.0 60000.0 16 -6.0 60000.0 60000.0 60000.0 17 -9.0 59500.0 60000.0 60000.0 18 -12.0 59500.0 60000.0 60000.0 19 -15.0 60000.0 59500.0 60000.0 20 -18.0 58500.0 59000.0 60000.0 21 -21.0 58500.0 58500.0 60000.0 22 -24.0 58000.0 58500.0 60000.0 23 -27.0 57500.0 58500.0 60000.0 24 -30.0 56500.0 58500.0 60000.0 25 -33.0 56000.0 58000.0 60000.0 26 -36.0 54500.0 57000.0 59000.0 27 -39.0 54000.0 56000.0 60000.0 28 -42.0 53500.0 56000.0 59000.0 29 -45.0 45500.0 55500.0 58500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)