DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0004/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0004/defocus_estimation/series0004_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0004/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 64000.0, 96000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 45.0 70000.0 90000.0 73500.0 1 42.0 96000.0 73500.0 96000.0 2 39.0 65500.0 66000.0 94500.0 3 36.0 64000.0 96000.0 96000.0 4 33.0 87500.0 66500.0 95500.0 5 30.0 96000.0 95000.0 95500.0 6 27.0 80500.0 96000.0 96000.0 7 24.0 76000.0 96000.0 96000.0 8 21.0 95000.0 95000.0 96000.0 9 18.0 96000.0 96000.0 96000.0 10 15.0 93500.0 94500.0 94500.0 11 12.0 94500.0 94000.0 92000.0 12 9.0 95000.0 93000.0 96000.0 13 6.0 93000.0 93000.0 94000.0 14 3.0 93500.0 94000.0 94000.0 15 0.1 92000.0 92500.0 91500.0 16 -0.0 94500.0 94500.0 93500.0 17 -3.0 93000.0 94500.0 94500.0 18 -6.0 93000.0 93000.0 95500.0 19 -9.0 94000.0 92500.0 93500.0 20 -12.0 93000.0 93000.0 92500.0 21 -15.0 79000.0 90000.0 92000.0 22 -18.0 90500.0 90500.0 91500.0 23 -21.0 89500.0 90000.0 91500.0 24 -24.0 87000.0 90000.0 91000.0 25 -27.0 83500.0 89500.0 91000.0 26 -30.0 86500.0 88500.0 89500.0 27 -33.0 87000.0 88500.0 90000.0 28 -36.0 84500.0 87500.0 89500.0 29 -39.0 85000.0 86500.0 89000.0 30 -42.0 83500.0 86500.0 88500.0 31 -45.0 81000.0 85000.0 87500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)