DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0006/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0006/defocus_estimation/series0006_tilts.txt Output diagnostic file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0006/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 64000.0, 96000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -45.0 64000.0 96000.0 96000.0 1 -42.0 95500.0 92000.0 95000.0 2 -39.0 96000.0 96000.0 96000.0 3 -36.0 66000.0 96000.0 95500.0 4 -33.0 96000.0 96000.0 96000.0 5 -30.0 96000.0 96000.0 94500.0 6 -27.0 96000.0 95000.0 93500.0 7 -24.0 95000.0 95500.0 94500.0 8 -21.0 85500.0 92000.0 93000.0 9 -18.0 93500.0 93000.0 94000.0 10 -15.0 93000.0 93000.0 91000.0 11 -12.0 92000.0 92000.0 90500.0 12 -9.0 83000.0 92000.0 91000.0 13 -6.0 65000.0 92000.0 92500.0 14 -3.0 92000.0 90500.0 91500.0 15 0.1 93000.0 92000.0 92000.0 16 3.0 83500.0 91000.0 91000.0 17 6.0 90000.0 91000.0 91000.0 18 9.0 66000.0 90500.0 90000.0 19 12.0 93500.0 89500.0 90000.0 20 15.0 89000.0 89500.0 90500.0 21 18.0 88000.0 88500.0 90000.0 22 21.0 85500.0 87000.0 89500.0 23 24.0 87000.0 87000.0 90000.0 24 27.0 86000.0 87500.0 89500.0 25 30.0 85000.0 85000.0 87500.0 26 33.0 82000.0 86500.0 87000.0 27 36.0 80000.0 83500.0 86000.0 28 39.0 77000.0 83500.0 83500.0 29 42.0 68500.0 83000.0 85500.0 30 45.0 71000.0 84000.0 85500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)