DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0007/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0007/defocus_estimation/series0007_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jchase/16sep28d/protomo_alignments/tiltseries0007/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 24000.0, 36000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 33.0 36000.0 34500.0 33000.0 1 30.0 36000.0 34000.0 33000.0 2 27.0 34000.0 33000.0 32000.0 3 24.0 34000.0 32000.0 31000.0 4 21.0 32000.0 31500.0 30500.0 5 15.0 30500.0 30500.0 28500.0 6 12.0 28500.0 30500.0 29500.0 7 9.0 29000.0 28500.0 29500.0 8 6.0 29000.0 29000.0 28500.0 9 3.0 28500.0 28500.0 28500.0 10 -0.1 28500.0 28500.0 28500.0 11 -3.0 28500.0 28500.0 28500.0 12 -6.0 27500.0 28500.0 28500.0 13 -9.0 28000.0 28000.0 28500.0 14 -12.0 29000.0 27500.0 28000.0 15 -15.0 27000.0 27000.0 27000.0 16 -18.0 26500.0 26500.0 28000.0 17 -21.0 25000.0 26000.0 27500.0 18 -24.0 25000.0 25500.0 26000.0 19 -27.0 24000.0 25500.0 28500.0 20 -30.0 32500.0 24500.0 26500.0 21 -33.0 36000.0 24000.0 26500.0 22 -36.0 35000.0 24000.0 25500.0 23 -39.0 31500.0 24500.0 25000.0 24 -42.0 33500.0 26500.0 24500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)