DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0002/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0002/defocus_estimation/series0002_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0002/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 36000.0, 54000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -45.0 37000.0 50500.0 46000.0 1 -42.0 45500.0 40000.0 36000.0 2 -39.0 37000.0 45500.0 45500.0 3 -36.0 36000.0 36500.0 44500.0 4 -33.0 39500.0 37500.0 53500.0 5 -30.0 39500.0 38500.0 48000.0 6 -27.0 37000.0 37000.0 39500.0 7 -24.0 52500.0 45500.0 36000.0 8 -21.0 36500.0 42500.0 51000.0 9 -18.0 54000.0 41000.0 36500.0 10 -15.0 38500.0 41500.0 37500.0 11 -12.0 41500.0 36000.0 38000.0 12 -9.0 41500.0 38000.0 37000.0 13 -6.0 53500.0 44000.0 41500.0 14 -3.0 45500.0 38000.0 38500.0 15 -0.1 42000.0 41500.0 38500.0 16 0.1 39000.0 37000.0 44500.0 17 3.0 49000.0 50000.0 54000.0 18 6.0 38000.0 53500.0 48500.0 19 9.0 36000.0 49000.0 41500.0 20 12.0 45500.0 44500.0 45000.0 21 15.0 38000.0 37500.0 54000.0 22 18.0 37500.0 54000.0 54000.0 23 21.0 43000.0 38500.0 39500.0 24 24.0 54000.0 39500.0 54000.0 25 27.0 54000.0 37500.0 36000.0 26 30.0 41000.0 49000.0 36500.0 27 33.0 53500.0 41500.0 37000.0 28 36.0 51500.0 36000.0 36000.0 29 39.0 54000.0 41500.0 42500.0 30 42.0 54000.0 38000.0 36500.0 31 45.0 48000.0 49000.0 40500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)