DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0007_thick300/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0007_thick300/defocus_estimation/series0007_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0007_thick300/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 84000.0, 126000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 42.0 108000.0 106500.0 103500.0 1 39.0 108000.0 106500.0 94500.0 2 36.0 109000.0 105000.0 105000.0 3 33.0 108000.0 108500.0 101500.0 4 30.0 109500.0 106000.0 105000.0 5 27.0 108500.0 105000.0 96500.0 6 24.0 108500.0 107500.0 110500.0 7 21.0 101000.0 109500.0 100500.0 8 18.0 110000.0 104000.0 106500.0 9 15.0 109000.0 100500.0 106500.0 10 12.0 112000.0 109000.0 93500.0 11 9.0 111000.0 108000.0 108500.0 12 6.0 111500.0 108000.0 85000.0 13 3.0 112500.0 108500.0 104000.0 14 0.1 112000.0 108500.0 92500.0 15 -0.1 109000.0 109500.0 111000.0 16 -3.0 110500.0 109000.0 109000.0 17 -6.0 110000.0 107500.0 115000.0 18 -9.0 114000.0 107500.0 109500.0 19 -12.0 97000.0 108500.0 115500.0 20 -15.0 108000.0 110500.0 113500.0 21 -18.0 107000.0 110000.0 109500.0 22 -21.0 107500.0 111000.0 116000.0 23 -24.0 108500.0 109500.0 109000.0 24 -27.0 106500.0 106500.0 94000.0 25 -30.0 105000.0 108000.0 104500.0 26 -33.0 107000.0 106000.0 107500.0 27 -36.0 105000.0 107500.0 111000.0 28 -39.0 104000.0 108000.0 112000.0 29 -42.0 98500.0 108000.0 108000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)