DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0008_thick200/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0008_thick200/defocus_estimation/series0008_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/17jul05d/protomo_alignments/tiltseries0008_thick200/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 48000.0, 72000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -42.0 63500.0 62000.0 59000.0 1 -39.0 64500.0 60500.0 49000.0 2 -36.0 64500.0 63500.0 60500.0 3 -33.0 64000.0 62500.0 59500.0 4 -30.0 64500.0 62500.0 57500.0 5 -27.0 65000.0 62500.0 52500.0 6 -24.0 63500.0 62500.0 61000.0 7 -21.0 64000.0 62500.0 60500.0 8 -18.0 64500.0 63500.0 61500.0 9 -15.0 66000.0 65500.0 64500.0 10 -12.0 66500.0 66000.0 64500.0 11 -9.0 67000.0 66500.0 65000.0 12 -6.0 66000.0 66500.0 52500.0 13 -3.0 62500.0 65500.0 62000.0 14 -0.1 66500.0 64000.0 63000.0 15 0.1 63500.0 63500.0 62500.0 16 3.0 65000.0 64000.0 65000.0 17 6.0 63500.0 63500.0 59000.0 18 9.0 62500.0 63500.0 60000.0 19 12.0 50000.0 63500.0 64500.0 20 15.0 61000.0 63000.0 65500.0 21 18.0 64500.0 65500.0 67000.0 22 21.0 66000.0 65000.0 63500.0 23 24.0 63000.0 64500.0 65000.0 24 27.0 62500.0 63000.0 66000.0 25 30.0 61000.0 63000.0 60500.0 26 33.0 60500.0 62000.0 61500.0 27 36.0 60500.0 62500.0 64500.0 28 39.0 61000.0 63000.0 64000.0 29 42.0 60500.0 63500.0 65500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)