DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0006/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0006/defocus_estimation/series0006_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0006/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 60.0 42000.0 35000.0 39000.0 1 57.0 33500.0 35000.0 36000.0 2 54.0 42000.0 32000.0 33000.0 3 51.0 41500.0 38000.0 34500.0 4 48.0 46000.0 34500.0 47500.0 5 45.0 35500.0 36500.0 33500.0 6 42.0 43500.0 48000.0 39000.0 7 39.0 40000.0 34500.0 35500.0 8 36.0 42500.0 40500.0 39000.0 9 33.0 36000.0 36000.0 35500.0 10 30.0 34000.0 33500.0 43000.0 11 27.0 40000.0 32000.0 36500.0 12 24.0 37500.0 46000.0 44000.0 13 21.0 35500.0 47500.0 48000.0 14 18.0 35500.0 33500.0 48000.0 15 15.0 33500.0 47500.0 46000.0 16 12.0 34000.0 43500.0 34000.0 17 9.0 46000.0 35500.0 48000.0 18 6.0 48000.0 46500.0 46500.0 19 3.0 46500.0 45500.0 46000.0 20 -0.1 46500.0 46000.0 46000.0 21 -3.0 46500.0 48000.0 46000.0 22 -6.0 47000.0 46000.0 45500.0 23 -9.0 46500.0 46000.0 45000.0 24 -12.0 45000.0 44500.0 44000.0 25 -15.0 46000.0 32000.0 42000.0 26 -18.0 32000.0 35500.0 43000.0 27 -21.0 38000.0 45000.0 41000.0 28 -24.0 38500.0 36000.0 41000.0 29 -27.0 39000.0 40500.0 38500.0 30 -30.0 34000.0 47500.0 38500.0 31 -33.0 38500.0 37500.0 38000.0 32 -36.0 47500.0 33000.0 35500.0 33 -39.0 32000.0 33000.0 36000.0 34 -42.0 32000.0 38500.0 33500.0 35 -45.0 42000.0 36500.0 34500.0 36 -48.0 37500.0 43000.0 32500.0 37 -51.0 38000.0 35000.0 35500.0 38 -54.0 33500.0 37500.0 47500.0 39 -57.0 47000.0 42500.0 40500.0 40 -60.0 33000.0 44000.0 47500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)