DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jan22i/protomo_alignments/tiltseries0002/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jan22i/protomo_alignments/tiltseries0002/defocus_estimation/series0002_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/17jan22i/protomo_alignments/tiltseries0002/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -39.0 42000.0 49500.0 40000.0 1 -36.0 44000.0 55000.0 58500.0 2 -33.0 59500.0 46500.0 59500.0 3 -30.0 58500.0 60000.0 60000.0 4 -27.0 44000.0 60000.0 59500.0 5 -24.0 60000.0 58000.0 59500.0 6 -21.0 59500.0 58500.0 59500.0 7 -18.0 58500.0 58000.0 58000.0 8 -15.0 58500.0 58000.0 56500.0 9 -12.0 57500.0 56500.0 56000.0 10 -9.0 56000.0 56000.0 56000.0 11 -6.0 56000.0 55500.0 56000.0 12 -3.0 55000.0 55000.0 55000.0 13 -0.1 54000.0 55000.0 55500.0 14 0.1 42000.0 42000.0 40000.0 15 3.0 60000.0 46500.0 40000.0 16 6.0 45500.0 40000.0 40500.0 17 9.0 40000.0 43500.0 42500.0 18 12.0 47000.0 42500.0 42000.0 19 15.0 60000.0 60000.0 60000.0 20 18.0 45000.0 48000.0 44000.0 21 21.0 60000.0 59500.0 59000.0 22 24.0 60000.0 59500.0 60000.0 23 27.0 60000.0 60000.0 58500.0 24 30.0 59500.0 60000.0 59500.0 25 33.0 57500.0 59500.0 58000.0 26 36.0 56500.0 57500.0 60000.0 27 39.0 55500.0 59000.0 60000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)