Loaded microscope plugin FEIScopePlugin.dll (named FEIScope) Greetings from NYSBC! This is the FEI Tecnai F20 with DE-20 Camera System at NYSBC. The last properties file change was 04/05/2016. SerialEM Version 3.5.4 64-bit, built Mar 7 2016 21:45:24 Started 9/14/2017 11:01:25 8 summed frames were saved to D:\RawFrames\DE20\20170914_99560_000_0p0 25 summed frames were saved to D:\RawFrames\DE20\20170914_99562_001_0p0 Min = 0.0, max = 15824.0, mean = 8526.09, SD = 802.35 Pixel size = 0.154 nm 54.161 electrons (8530.35 counts) per unbinned pixel per second 25 summed frames were saved to D:\RawFrames\DE20\20170914_99563_002_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99576_gain_003_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99577_gain_004_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99578_gain_005_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99579_gain_006_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99580_gain_007_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99581_gain_008_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99582_gain_009_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99583_gain_010_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99584_gain_011_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99585_gain_012_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99586_gain_013_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99587_gain_014_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99588_gain_015_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99589_gain_016_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99590_gain_017_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99591_gain_018_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99592_gain_019_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99593_gain_020_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99594_gain_021_0p0 1 summed frames were saved to D:\RawFrames\DE20\20170914_99595_gain_022_0p0 Dose rate calculated to be 23.03 electrons/square Angstrom/sec for spot 3, C2 50.36% 54.62 electrons/unbinned pixel/sec 1 summed frames were saved to D:\RawFrames\DE20\20170914_99595_gain_022_0p0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99596_023_0p0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99598_024_0p0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99599_025_0p0 Measured defocus = 26.39 microns drift = 0.75 nm/sec Measured defocus = 6.66 microns drift = 0.30 nm/sec Measured defocus = -7.11 microns drift = 0.22 nm/sec 8 summed frames were saved to D:\RawFrames\DE20\20170914_99601_026_0p0 Rough eucentricity: changing Z by -21.71 to 71.88, continuing... Rough eucentricity: changing Z by -1.19 to 70.69, finished. Measured defocus = 0.00 microns drift = 0.00 nm/sec Measured defocus = -36.32 microns drift = 0.10 nm/sec Measured defocus = -16.93 microns drift = 0.07 nm/sec Measured defocus = -1.25 microns drift = 0.04 nm/sec Measured defocus = -4.31 microns drift = 0.17 nm/sec 8 summed frames were saved to D:\RawFrames\DE20\20170914_99602_027_0p0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99603_028_0p0 Rough eucentricity: changing Z by -4.94 to 88.64, finished. Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 1.12 microns and lateral displacement of 4.29 microns Measured defocus = -4.72 microns drift = 1.51 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.075 um; position 0.036, 0.107 Measured defocus = -3.95 microns drift = 0.84 nm/sec Tracking image shifted -0.004, 0.018 um; position 0.040, 0.088 8 summed frames were saved to D:\RawFrames\DE20\20170914_99607_tomo1_000_18p0 Saved Z = 0 at 0.00 min Tilt = 18.00 Mean = 2397 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.040 0.088 0.627 Need regular tracking - Need autofocus Tracking image shifted 0.021, 0.106 um; position 0.018, -0.018 Measured defocus = -3.94 microns drift = 0.17 nm/sec Tracking image shifted -0.003, 0.002 um; position 0.022, -0.020 8 summed frames were saved to D:\RawFrames\DE20\20170914_99608_tomo1_001_15p1 Record image shifted -0.002, -0.019 um Tracking reference shifted by 19.9,-2.6 pixels, -0.002,-0.019 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 1 at 0.76 min Tilt = 15.15 Mean = 2395 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.024 -0.001 0.572 Predicted X = 0.009 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.090 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 0.517 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.003 um; position 0.013, -0.088 Measured defocus = -4.04 microns drift = 0.21 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.015, -0.086 8 summed frames were saved to D:\RawFrames\DE20\20170914_99609_tomo1_002_12p3 Record image shifted -0.001, -0.009 um Tracking reference shifted by 9.7,-0.8 pixels, -0.001,-0.009 um Saved Z = 2 at 1.55 min Tilt = 12.25 Mean = 2402 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.016 -0.077 0.561 Errors in X/Y Prediction 0.007 0.013 um, 1.0 1.8 % Error in Z Prediction 0.044 Predicted X = 0.007 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.162 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Z = 0.521 StdErr = 0.033 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.007 um; position 0.003, -0.155 8 summed frames were saved to D:\RawFrames\DE20\20170914_99610_tomo1_003_9p3 Record image shifted 0.001, -0.009 um Tracking reference shifted by 9.6,1.6 pixels, 0.001,-0.009 um Saved Z = 3 at 2.04 min Tilt = 9.32 Mean = 2400 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.002 -0.146 0.521 Errors in X/Y Prediction -0.005 0.016 um, 0.7 2.2 % Predicted X = -0.012 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.220 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Z = 0.489 StdErr = 0.044 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, 0.126 um; position -0.029, -0.347 Measured defocus = -4.25 microns drift = 0.81 nm/sec Tracking image shifted 0.022, 0.014 um; position -0.051, -0.361 8 summed frames were saved to D:\RawFrames\DE20\20170914_99611_tomo1_004_6p4 Record image shifted 0.008, -0.018 um Tracking reference shifted by 18.8,8.5 pixels, 0.008,-0.018 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 4 at 2.81 min Tilt = 6.36 Mean = 2403 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.059 -0.343 0.736 Errors in X/Y Prediction -0.047 -0.123 um, 6.3 16.5 % Error in Z Prediction 0.247 Predicted X = -0.119 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.455 StdErr = 0.095 nFit = 3 nDrop = 0 Predicted Z = 0.782 StdErr = 0.088 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.059, -0.045 um; position -0.061, -0.410 Measured defocus = -3.93 microns drift = 0.40 nm/sec Tracking image shifted 0.011, -0.006 um; position -0.072, -0.404 8 summed frames were saved to D:\RawFrames\DE20\20170914_99612_tomo1_005_3p4 Record image shifted 0.022, -0.010 um Tracking reference shifted by 11.0,23.0 pixels, 0.022,-0.010 um Saved Z = 5 at 3.60 min Tilt = 3.38 Mean = 2400 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.093 -0.393 0.713 Errors in X/Y Prediction 0.026 0.062 um, 3.5 8.3 % Error in Z Prediction -0.069 Predicted X = -0.128 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.502 StdErr = 0.181 nFit = 4 nDrop = 0 Predicted Z = 0.800 StdErr = 0.093 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.020, -0.045 um; position -0.108, -0.457 8 summed frames were saved to D:\RawFrames\DE20\20170914_99613_tomo1_006_0p4 Record image shifted -0.004, -0.014 um Tracking reference shifted by 14.5,-4.0 pixels, -0.004,-0.014 um Saved Z = 6 at 4.09 min Tilt = 0.38 Mean = 2401 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.105 -0.444 0.800 Errors in X/Y Prediction 0.024 0.059 um, 3.2 7.9 % Predicted X = -0.116 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.384 StdErr = 0.097 nFit = 4 nDrop = 0 Predicted Z = 0.856 StdErr = 0.114 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.015, 0.119 um; position -0.101, -0.503 Measured defocus = -3.84 microns drift = 0.32 nm/sec Tracking image shifted 0.010, -0.009 um; position -0.111, -0.494 8 summed frames were saved to D:\RawFrames\DE20\20170914_99614_tomo1_007_-2p6 Record image shifted -0.002, -0.011 um Tracking reference shifted by 11.4,-2.3 pixels, -0.002,-0.011 um Saved Z = 7 at 4.86 min Tilt = -2.62 Mean = 2404 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.109 -0.483 0.697 Errors in X/Y Prediction 0.007 -0.099 um, 0.9 13.4 % Error in Z Prediction -0.160 Predicted X = -0.113 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.520 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 0.682 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.046 um; position -0.111, -0.566 8 summed frames were saved to D:\RawFrames\DE20\20170914_99615_tomo1_008_-5p6 Record image shifted -0.000, -0.012 um Tracking reference shifted by 13.2,-0.3 pixels, -0.000,-0.012 um Saved Z = 8 at 5.34 min Tilt = -5.61 Mean = 2405 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.111 -0.553 0.682 Errors in X/Y Prediction 0.002 -0.033 um, 0.2 4.5 % Predicted X = -0.114 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.623 StdErr = 0.027 nFit = 4 nDrop = 0 Predicted Z = 0.670 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, 0.011 um; position -0.130, -0.634 8 summed frames were saved to D:\RawFrames\DE20\20170914_99616_tomo1_009_-8p6 Record image shifted -0.000, -0.008 um Tracking reference shifted by 9.0,-0.5 pixels, -0.000,-0.008 um Saved Z = 9 at 5.82 min Tilt = -8.60 Mean = 2403 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.130 -0.626 0.670 Errors in X/Y Prediction -0.016 -0.002 um, 2.2 0.3 % Predicted X = -0.148 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.721 StdErr = 0.019 nFit = 4 nDrop = 0 Predicted Z = 0.657 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, -0.023 um; position -0.144, -0.698 8 summed frames were saved to D:\RawFrames\DE20\20170914_99617_tomo1_010_-11p6 Record image shifted -0.003, -0.009 um Tracking reference shifted by 9.4,-3.1 pixels, -0.003,-0.009 um Saved Z = 10 at 6.31 min Tilt = -11.56 Mean = 2401 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.141 -0.690 0.657 Errors in X/Y Prediction 0.007 0.032 um, 0.9 4.3 % Predicted X = -0.153 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.753 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 0.645 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.001 um; position -0.151, -0.752 Measured defocus = -3.87 microns drift = 0.84 nm/sec Tracking image shifted 0.005, -0.009 um; position -0.156, -0.743 8 summed frames were saved to D:\RawFrames\DE20\20170914_99618_tomo1_011_-14p5 Record image shifted -0.001, -0.002 um Tracking reference shifted by 2.1,-1.4 pixels, -0.001,-0.002 um Saved Z = 11 at 7.08 min Tilt = -14.50 Mean = 2403 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.154 -0.741 0.519 Errors in X/Y Prediction -0.002 0.012 um, 0.3 1.6 % Error in Z Prediction -0.126 Predicted X = -0.168 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.783 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Z = 0.495 StdErr = 0.059 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.024 um; position -0.162, -0.807 8 summed frames were saved to D:\RawFrames\DE20\20170914_99619_tomo1_012_-17p4 Record image shifted -0.001, -0.018 um Tracking reference shifted by 18.7,-1.5 pixels, -0.001,-0.018 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 12 at 7.57 min Tilt = -17.40 Mean = 2401 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.160 -0.789 0.495 Errors in X/Y Prediction 0.008 -0.007 um, 1.0 0.9 % Predicted X = -0.166 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.827 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 0.461 StdErr = 0.066 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99620_tomo1_013_-20p3 Record image shifted -0.003, 0.009 um Saved Z = 13 at 8.06 min Tilt = -20.27 Mean = 2394 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.163 -0.836 0.461 Errors in X/Y Prediction 0.003 -0.009 um, 0.4 1.2 % Predicted X = -0.165 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.881 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Z = 0.427 StdErr = 0.072 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99621_tomo1_014_-23p1 Record image shifted -0.007, 0.023 um Saved Z = 14 at 8.15 min Tilt = -23.08 Mean = 2395 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.158 -0.904 0.427 Errors in X/Y Prediction 0.007 -0.023 um, 0.9 3.2 % Predicted X = -0.153 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.976 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Z = 0.393 StdErr = 0.080 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.035 um; position -0.152, -0.941 Measured defocus = -3.98 microns drift = 0.23 nm/sec Tracking image shifted 0.008, -0.004 um; position -0.160, -0.937 8 summed frames were saved to D:\RawFrames\DE20\20170914_99622_tomo1_015_-25p8 Record image shifted 0.010, -0.019 um Tracking reference shifted by 20.4,10.2 pixels, 0.010,-0.019 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 15 at 8.92 min Tilt = -25.83 Mean = 2392 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.169 -0.918 0.377 Errors in X/Y Prediction -0.016 0.058 um, 2.1 7.8 % Error in Z Prediction -0.016 Predicted X = -0.180 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.935 StdErr = 0.050 nFit = 4 nDrop = 0 Predicted Z = 0.331 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.015, 0.053 um; position -0.165, -0.987 8 summed frames were saved to D:\RawFrames\DE20\20170914_99623_tomo1_016_-28p5 Record image shifted 0.024, 0.011 um Tracking reference shifted by -11.2,25.3 pixels, 0.024,0.011 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 16 at 9.44 min Tilt = -28.53 Mean = 2381 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.189 -0.998 0.331 Errors in X/Y Prediction -0.009 -0.063 um, 1.2 8.5 % Predicted X = -0.209 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.053 StdErr = 0.079 nFit = 4 nDrop = 0 Predicted Z = 0.291 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.026, -0.012 um; position -0.183, -1.041 8 summed frames were saved to D:\RawFrames\DE20\20170914_99624_tomo1_017_-31p2 Record image shifted 0.013, -0.060 um Tracking reference shifted by 64.2,14.1 pixels, 0.013,-0.060 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 17 at 9.95 min Tilt = -31.17 Mean = 2386 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.196 -0.980 0.291 Errors in X/Y Prediction 0.012 0.072 um, 1.7 9.7 % Predicted X = -0.212 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.988 StdErr = 0.107 nFit = 4 nDrop = 0 Predicted Z = 0.251 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.022, 0.040 um; position -0.190, -1.029 8 summed frames were saved to D:\RawFrames\DE20\20170914_99625_tomo1_018_-33p7 Record image shifted 0.007, -0.053 um Tracking reference shifted by 56.3,7.0 pixels, 0.007,-0.053 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 18 at 10.46 min Tilt = -33.74 Mean = 2379 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.197 -0.976 0.251 Errors in X/Y Prediction 0.015 0.012 um, 2.1 1.7 % Predicted X = -0.202 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.902 StdErr = 0.072 nFit = 4 nDrop = 0 Predicted Z = 0.211 StdErr = 0.027 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, 0.115 um; position -0.189, -1.018 Measured defocus = -3.93 microns drift = 1.05 nm/sec Tracking image shifted 0.001, -0.028 um; position -0.191, -0.990 8 summed frames were saved to D:\RawFrames\DE20\20170914_99626_tomo1_019_-36p2 Record image shifted 0.009, -0.016 um Tracking reference shifted by 17.0,9.3 pixels, 0.009,-0.016 um Saved Z = 19 at 11.26 min Tilt = -36.23 Mean = 2367 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.199 -0.974 0.157 Errors in X/Y Prediction 0.003 -0.072 um, 0.4 9.7 % Error in Z Prediction -0.054 Predicted X = -0.200 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.982 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 0.125 StdErr = 0.046 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.007 um; position -0.199, -0.989 8 summed frames were saved to D:\RawFrames\DE20\20170914_99627_tomo1_020_-38p6 Record image shifted -0.001, -0.002 um Tracking reference shifted by 2.0,-1.0 pixels, -0.001,-0.002 um Saved Z = 20 at 11.74 min Tilt = -38.65 Mean = 2356 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.199 -0.987 0.125 Errors in X/Y Prediction 0.002 -0.005 um, 0.2 0.7 % Predicted X = -0.200 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -1.006 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Z = 0.079 StdErr = 0.050 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99628_tomo1_021_-41p0 Record image shifted -0.009, 0.015 um Saved Z = 21 at 11.82 min Tilt = -41.00 Mean = 2353 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.191 -1.021 0.079 Errors in X/Y Prediction 0.009 -0.015 um, 1.2 2.0 % Predicted X = -0.188 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.071 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Z = 0.034 StdErr = 0.054 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.078 um; position -0.192, -0.994 8 summed frames were saved to D:\RawFrames\DE20\20170914_99629_tomo1_022_-43p3 Record image shifted 0.002, -0.002 um Tracking reference shifted by 2.0,1.9 pixels, 0.002,-0.002 um Saved Z = 22 at 12.30 min Tilt = -43.26 Mean = 2340 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.193 -0.992 0.034 Measured defocus = -4.45 microns drift = 2.02 nm/sec Errors in X/Y Prediction -0.005 0.080 um, 0.7 10.7 % Need regular tracking - Need autofocus Tracking image shifted -0.041, -0.057 um; position -0.210, 0.134 Measured defocus = -4.01 microns drift = 1.96 nm/sec Tracking image shifted -0.015, 0.086 um; position -0.195, 0.048 8 summed frames were saved to D:\RawFrames\DE20\20170914_99630_tomo1_023_20p9 Record image shifted -0.002, 0.050 um Tracking reference shifted by -53.3,-2.5 pixels, -0.002,0.050 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 23 at 14.04 min Tilt = 20.85 Mean = 2390 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.193 -0.002 0.530 Need regular tracking - Need autofocus Tracking image shifted -0.012, -0.053 um; position -0.181, 0.051 Measured defocus = -4.02 microns drift = 1.56 nm/sec Tracking image shifted -0.004, 0.076 um; position -0.177, -0.025 8 summed frames were saved to D:\RawFrames\DE20\20170914_99631_tomo1_024_23p7 Record image shifted -0.009, 0.063 um Tracking reference shifted by -67.2,-9.5 pixels, -0.009,0.063 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 24 at 14.85 min Tilt = 23.66 Mean = 2383 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.168 -0.088 0.581 Predicted X = -0.144 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.174 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 0.633 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.016, -0.125 um; position -0.160, -0.050 Measured defocus = -3.96 microns drift = 2.16 nm/sec Tracking image shifted 0.005, 0.089 um; position -0.165, -0.139 8 summed frames were saved to D:\RawFrames\DE20\20170914_99632_tomo1_025_26p4 Record image shifted -0.002, 0.057 um Tracking reference shifted by -60.5,-2.2 pixels, -0.002,0.057 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 25 at 15.64 min Tilt = 26.40 Mean = 2380 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.163 -0.196 0.637 Errors in X/Y Prediction -0.019 -0.021 um, 2.5 2.9 % Error in Z Prediction 0.004 Predicted X = -0.157 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.289 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Z = 0.691 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, -0.124 um; position -0.157, -0.165 8 summed frames were saved to D:\RawFrames\DE20\20170914_99633_tomo1_026_29p1 Record image shifted 0.007, 0.125 um Tracking reference shifted by -133.4,8.1 pixels, 0.007,0.125 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 26 at 16.15 min Tilt = 29.09 Mean = 2360 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.164 -0.290 0.691 Errors in X/Y Prediction -0.007 -0.001 um, 1.0 0.2 % Predicted X = -0.165 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.394 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Z = 0.744 StdErr = 0.004 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99634_tomo1_027_31p7 Record image shifted 0.014, 0.100 um Saved Z = 27 at 16.64 min Tilt = 31.71 Mean = 2357 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.180 -0.493 0.744 Errors in X/Y Prediction -0.014 -0.100 um, 1.9 13.4 % Predicted X = -0.186 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.624 StdErr = 0.081 nFit = 3 nDrop = 0 Predicted Z = 0.798 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.272 um; position -0.199, -0.896 Measured defocus = -3.97 microns drift = 0.61 nm/sec Tracking image shifted -0.013, 0.014 um; position -0.187, -0.910 8 summed frames were saved to D:\RawFrames\DE20\20170914_99635_tomo1_028_34p3 Record image shifted -0.001, 0.081 um Tracking reference shifted by -87.0,-1.4 pixels, -0.001,0.081 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 28 at 17.42 min Tilt = 34.26 Mean = 2362 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.185 -0.991 0.774 Errors in X/Y Prediction 0.001 -0.367 um, 0.1 49.5 % Error in Z Prediction -0.025 Predicted X = -0.198 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -1.645 StdErr = 0.123 nFit = 4 nDrop = 0 Predicted Z = 0.823 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.555, 0.282 um; position -0.752, -1.926 8 summed frames were saved to D:\RawFrames\DE20\20170914_99636_tomo1_029_36p7 Record image shifted 0.813, 0.142 um Tracking reference shifted by -150.1,869.1 pixels, 0.813,0.142 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.697 in X, 0.153 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99637_tomo1_030_36p7 Record image shifted -0.252, 0.206 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.216 in X, 0.227 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99638_tomo1_031_36p7 Record image shifted -0.024, 0.068 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 29 at 20.57 min Tilt = 36.74 Mean = 2292 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -1.290 -2.342 0.823 Resetting image shifts Errors in X/Y Prediction -1.092 -0.697 um, 147.2 94.0 % Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.152 um; position -0.142, -0.057 8 summed frames were saved to D:\RawFrames\DE20\20170914_99639_tomo1_032_31p7 Record image shifted -0.174, -0.116 um Saved Z = 27 at 23.48 min Tilt = 31.71 Mean = 2412 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.174 0.116 -0.220 Need regular tracking - Need autofocus Tracking image shifted 0.838, 0.328 um; position -0.665, -0.212 Measured defocus = -4.44 microns drift = 0.15 nm/sec Tracking image shifted 0.657, 0.094 um; position -1.321, -0.306 8 summed frames were saved to D:\RawFrames\DE20\20170914_99640_tomo1_033_34p3 Record image shifted -0.113, -0.127 um Tracking reference shifted by 135.1,-121.1 pixels, -0.113,-0.127 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 28 at 24.52 min Tilt = 34.26 Mean = 2308 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -1.208 -0.179 0.280 Predicted X = -2.590 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.474 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.399, 0.011 um; position -2.989, -0.486 Measured defocus = -4.64 microns drift = 0.94 nm/sec Tracking image shifted 0.311, -0.005 um; position -3.300, -0.481 8 summed frames were saved to D:\RawFrames\DE20\20170914_99641_tomo1_034_36p7 Record image shifted -0.690, -0.464 um Tracking reference shifted by 495.0,-738.3 pixels, -0.690,-0.464 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.591 in X, 0.524 in Y) 8 summed frames were saved to D:\RawFrames\DE20\20170914_99642_tomo1_035_36p7 Record image shifted -0.111, -0.157 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.095 in X, 0.171 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99643_tomo1_036_36p7 Record image shifted -0.109, 0.090 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 29 at 29.10 min Tilt = 36.74 Mean = 2248 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -2.389 0.051 0.921 Errors in X/Y Prediction 0.201 0.526 um, 27.1 70.8 % Predicted X = -3.704 StdErr = 0.150 nFit = 3 nDrop = 0 Predicted Y = -0.069 StdErr = 0.392 nFit = 3 nDrop = 0 Predicted Z = 1.562 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.522, -0.220 um; position -3.183, 0.152 Measured defocus = -3.18 microns drift = 0.45 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.15 microns drift = 0.28 nm/sec Tracking image shifted -0.338, 0.053 um; position -2.845, 0.098 8 summed frames were saved to D:\RawFrames\DE20\20170914_99644_tomo1_037_39p1 Record image shifted -0.457, 0.035 um Tracking reference shifted by -38.5,-488.6 pixels, -0.457,0.035 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.392 in X, 0.023 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99645_tomo1_038_39p1 Record image shifted -0.006, 0.007 um Saved Z = 30 at 30.09 min Tilt = 39.14 Mean = 2243 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -2.381 0.056 0.941 Errors in X/Y Prediction 1.323 0.125 um, 178.3 16.8 % Error in Z Prediction -0.621 Predicted X = -3.166 StdErr = 0.886 nFit = 3 nDrop = 0 Predicted Y = 0.211 StdErr = 0.168 nFit = 3 nDrop = 0 Predicted Z = 1.375 StdErr = 0.463 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.464, 0.094 um; position -2.701, 0.117 Measured defocus = -2.98 microns drift = 0.25 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.18 microns drift = 0.14 nm/sec Tracking image shifted -0.331, 0.013 um; position -2.371, 0.105 8 summed frames were saved to D:\RawFrames\DE20\20170914_99646_tomo1_039_41p5 Record image shifted 0.002, 0.043 um Tracking reference shifted by -45.6,1.8 pixels, 0.002,0.043 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 31 at 30.98 min Tilt = 41.47 Mean = 2231 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -2.372 0.062 0.550 Errors in X/Y Prediction 0.793 -0.149 um, 106.9 20.1 % Error in Z Prediction -0.825 Predicted X = -2.364 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.067 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Z = 0.433 StdErr = 0.306 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, -0.042 um; position -2.360, 0.109 Measured defocus = -3.87 microns drift = 0.29 nm/sec Tracking image shifted -0.004, 0.007 um; position -2.357, 0.102 8 summed frames were saved to D:\RawFrames\DE20\20170914_99647_tomo1_040_43p7 Record image shifted 0.006, 0.038 um Tracking reference shifted by -40.7,6.8 pixels, 0.006,0.038 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 32 at 31.78 min Tilt = 43.71 Mean = 2220 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -2.363 0.064 0.310 2017/09/14 12:02 D:\serialem\hwei\091417\tomo1.st 0 33 3712 4984 1221 18.0 43.7 3.00 COS 31.8 1x1 DE20 19000 1 0.23 LD 0.0 0.0 Total dose in electrons/square Angstrom: Recording: 105.0 Tracking: 352.3 Focusing: 352.3 Tasks: 22.47 Previews: 0.0 Measured defocus = -5.02 microns drift = 0.49 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.010, 0.077 um; position 0.084, 0.753 Measured defocus = -3.86 microns drift = 0.61 nm/sec Tracking image shifted -0.002, 0.013 um; position 0.086, 0.740 8 summed frames were saved to D:\RawFrames\DE20\20170914_99649_tomo2_000_18p0 Saved Z = 0 at 0.00 min Tilt = 18.00 Mean = 2322 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.086 0.740 1.201 Need regular tracking - Need autofocus Tracking image shifted 0.030, 0.130 um; position 0.056, 0.610 Measured defocus = -4.01 microns drift = 0.10 nm/sec Tracking image shifted -0.001, -0.000 um; position 0.057, 0.610 8 summed frames were saved to D:\RawFrames\DE20\20170914_99650_tomo2_001_15p1 Record image shifted -0.004, -0.035 um Tracking reference shifted by 37.6,-3.9 pixels, -0.004,-0.035 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 1 at 0.76 min Tilt = 15.15 Mean = 2330 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.060 0.645 1.214 Predicted X = 0.035 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.551 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.227 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, 0.022 um; position 0.046, 0.529 Measured defocus = -3.95 microns drift = 0.23 nm/sec Tracking image shifted -0.005, -0.014 um; position 0.051, 0.543 8 summed frames were saved to D:\RawFrames\DE20\20170914_99651_tomo2_002_12p3 Record image shifted -0.004, -0.031 um Tracking reference shifted by 33.4,-4.8 pixels, -0.004,-0.031 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 2 at 1.55 min Tilt = 12.25 Mean = 2334 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.056 0.574 1.171 Errors in X/Y Prediction 0.021 0.023 um, 2.8 3.1 % Error in Z Prediction -0.056 Predicted X = 0.051 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.487 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Z = 1.165 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.017 um; position 0.047, 0.470 8 summed frames were saved to D:\RawFrames\DE20\20170914_99652_tomo2_003_9p3 Record image shifted -0.004, -0.043 um Tracking reference shifted by 46.2,-4.8 pixels, -0.004,-0.043 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 3 at 2.06 min Tilt = 9.32 Mean = 2338 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.052 0.514 1.165 Errors in X/Y Prediction 0.001 0.027 um, 0.1 3.6 % Predicted X = 0.048 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.446 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Z = 1.150 StdErr = 0.056 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.029 um; position 0.048, 0.417 Measured defocus = -4.07 microns drift = 0.53 nm/sec Tracking image shifted -0.002, -0.014 um; position 0.050, 0.431 8 summed frames were saved to D:\RawFrames\DE20\20170914_99653_tomo2_004_6p4 Record image shifted -0.002, -0.037 um Tracking reference shifted by 39.3,-2.7 pixels, -0.002,-0.037 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 4 at 2.86 min Tilt = 6.36 Mean = 2341 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.053 0.467 1.223 Errors in X/Y Prediction 0.005 0.021 um, 0.7 2.9 % Error in Z Prediction 0.073 Predicted X = 0.053 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.412 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Z = 1.219 StdErr = 0.063 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.039 um; position 0.054, 0.373 8 summed frames were saved to D:\RawFrames\DE20\20170914_99654_tomo2_005_3p4 Record image shifted -0.003, -0.043 um Tracking reference shifted by 45.5,-2.9 pixels, -0.003,-0.043 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 5 at 3.36 min Tilt = 3.38 Mean = 2346 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.057 0.415 1.219 Errors in X/Y Prediction 0.003 0.003 um, 0.4 0.5 % Predicted X = 0.061 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.372 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Z = 1.225 StdErr = 0.076 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.057 um; position 0.064, 0.315 8 summed frames were saved to D:\RawFrames\DE20\20170914_99655_tomo2_006_0p4 Record image shifted -0.001, -0.045 um Tracking reference shifted by 48.1,-1.5 pixels, -0.001,-0.045 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 6 at 3.86 min Tilt = 0.38 Mean = 2346 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.066 0.360 1.225 Errors in X/Y Prediction 0.005 -0.012 um, 0.7 1.7 % Predicted X = 0.075 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.299 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Z = 1.231 StdErr = 0.091 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, 0.033 um; position 0.087, 0.266 Measured defocus = -3.96 microns drift = 0.35 nm/sec Tracking image shifted 0.002, -0.016 um; position 0.085, 0.282 8 summed frames were saved to D:\RawFrames\DE20\20170914_99657_tomo2_007_-2p6 Record image shifted -0.001, -0.034 um Tracking reference shifted by 36.4,-1.0 pixels, -0.001,-0.034 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 7 at 4.67 min Tilt = -2.62 Mean = 2352 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.086 0.316 1.191 Errors in X/Y Prediction 0.012 0.017 um, 1.6 2.3 % Error in Z Prediction -0.041 Predicted X = 0.107 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.272 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Z = 1.205 StdErr = 0.055 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.080 um; position 0.104, 0.192 8 summed frames were saved to D:\RawFrames\DE20\20170914_99658_tomo2_008_-5p6 Record image shifted 0.003, -0.036 um Tracking reference shifted by 38.2,3.4 pixels, 0.003,-0.036 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 8 at 5.17 min Tilt = -5.61 Mean = 2355 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.101 0.228 1.205 Errors in X/Y Prediction -0.006 -0.045 um, 0.9 6.0 % Predicted X = 0.115 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.137 StdErr = 0.037 nFit = 4 nDrop = 0 Predicted Z = 1.208 StdErr = 0.062 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.019, 0.051 um; position 0.096, 0.086 8 summed frames were saved to D:\RawFrames\DE20\20170914_99659_tomo2_009_-8p6 Record image shifted 0.031, 0.100 um Tracking reference shifted by -106.7,33.6 pixels, 0.031,0.100 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 9 at 5.68 min Tilt = -8.60 Mean = 2348 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.065 -0.014 1.208 Errors in X/Y Prediction -0.051 -0.151 um, 6.8 20.4 % Predicted X = 0.028 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.327 StdErr = 0.072 nFit = 4 nDrop = 0 Predicted Z = 1.211 StdErr = 0.070 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.025, -0.181 um; position 0.054, -0.146 8 summed frames were saved to D:\RawFrames\DE20\20170914_99660_tomo2_010_-11p6 Record image shifted 0.031, -0.028 um Tracking reference shifted by 30.3,32.9 pixels, 0.031,-0.028 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 10 at 6.19 min Tilt = -11.56 Mean = 2355 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.023 -0.118 1.211 Errors in X/Y Prediction -0.006 0.209 um, 0.7 28.1 % Predicted X = -0.019 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.303 StdErr = 0.192 nFit = 4 nDrop = 0 Predicted Z = 1.213 StdErr = 0.079 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.036, -0.043 um; position 0.017, -0.259 Measured defocus = -4.09 microns drift = 0.49 nm/sec Tracking image shifted 0.011, -0.023 um; position 0.006, -0.237 8 summed frames were saved to D:\RawFrames\DE20\20170914_99661_tomo2_011_-14p5 Record image shifted 0.020, -0.034 um Tracking reference shifted by 36.6,21.1 pixels, 0.020,-0.034 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 11 at 6.98 min Tilt = -14.50 Mean = 2350 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.014 -0.202 1.307 Errors in X/Y Prediction 0.005 0.101 um, 0.6 13.6 % Error in Z Prediction 0.093 Predicted X = -0.051 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.179 StdErr = 0.078 nFit = 4 nDrop = 0 Predicted Z = 1.300 StdErr = 0.083 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.033, 0.147 um; position -0.018, -0.326 8 summed frames were saved to D:\RawFrames\DE20\20170914_99662_tomo2_012_-17p4 Record image shifted 0.012, -0.038 um Tracking reference shifted by 41.0,13.1 pixels, 0.012,-0.038 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 12 at 7.48 min Tilt = -17.41 Mean = 2350 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.031 -0.288 1.300 Errors in X/Y Prediction 0.020 -0.109 um, 2.7 14.7 % Predicted X = -0.047 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.358 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Z = 1.313 StdErr = 0.090 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.016, 0.055 um; position -0.032, -0.413 8 summed frames were saved to D:\RawFrames\DE20\20170914_99663_tomo2_013_-20p3 Record image shifted 0.012, -0.050 um Tracking reference shifted by 53.4,13.1 pixels, 0.012,-0.050 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 13 at 7.99 min Tilt = -20.27 Mean = 2348 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.044 -0.363 1.313 Errors in X/Y Prediction 0.003 -0.005 um, 0.4 0.6 % Predicted X = -0.058 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.436 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 1.326 StdErr = 0.098 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99664_tomo2_014_-23p1 Record image shifted -0.001, 0.008 um Saved Z = 14 at 8.47 min Tilt = -23.09 Mean = 2337 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.056 -0.444 1.326 Errors in X/Y Prediction 0.001 -0.008 um, 0.2 1.0 % Predicted X = -0.068 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.519 StdErr = 0.011 nFit = 4 nDrop = 0 Predicted Z = 1.339 StdErr = 0.107 nFit = 3 nDrop = 0 Need autofocus Measured defocus = -3.85 microns drift = 0.20 nm/sec 8 summed frames were saved to D:\RawFrames\DE20\20170914_99665_tomo2_015_-25p8 Record image shifted 0.002, -0.008 um Saved Z = 15 at 9.00 min Tilt = -25.84 Mean = 2340 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.071 -0.511 1.187 Errors in X/Y Prediction -0.002 0.008 um, 0.3 1.0 % Error in Z Prediction -0.152 Predicted X = -0.085 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.579 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Z = 1.226 StdErr = 0.140 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.009, 0.089 um; position -0.076, -0.668 Measured defocus = -3.92 microns drift = 0.20 nm/sec Tracking image shifted 0.007, -0.011 um; position -0.083, -0.657 8 summed frames were saved to D:\RawFrames\DE20\20170914_99666_tomo2_016_-28p5 Record image shifted 0.007, -0.075 um Tracking reference shifted by 80.6,7.5 pixels, 0.007,-0.075 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 16 at 9.76 min Tilt = -28.54 Mean = 2340 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.090 -0.581 1.156 Errors in X/Y Prediction -0.005 -0.002 um, 0.6 0.3 % Error in Z Prediction -0.070 Predicted X = -0.109 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.643 StdErr = 0.011 nFit = 4 nDrop = 0 Predicted Z = 1.127 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.020, 0.044 um; position -0.089, -0.686 8 summed frames were saved to D:\RawFrames\DE20\20170914_99667_tomo2_017_-31p2 Record image shifted 0.010, -0.043 um Tracking reference shifted by 45.5,10.2 pixels, 0.010,-0.043 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 17 at 10.27 min Tilt = -31.18 Mean = 2336 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.099 -0.644 1.127 Errors in X/Y Prediction 0.010 -0.001 um, 1.4 0.2 % Predicted X = -0.115 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.706 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Z = 1.097 StdErr = 0.000 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99668_tomo2_018_-33p7 Record image shifted -0.009, -0.007 um Saved Z = 18 at 10.75 min Tilt = -33.75 Mean = 2318 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.106 -0.699 1.097 Errors in X/Y Prediction 0.009 0.007 um, 1.1 1.0 % Predicted X = -0.115 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.746 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Z = 1.067 StdErr = 0.000 nFit = 3 nDrop = 0 8 summed frames were saved to D:\RawFrames\DE20\20170914_99669_tomo2_019_-36p2 Record image shifted -0.010, 0.031 um Saved Z = 19 at 10.83 min Tilt = -36.24 Mean = 2315 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.104 -0.778 1.067 Errors in X/Y Prediction 0.010 -0.031 um, 1.4 4.2 % Predicted X = -0.109 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.857 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Z = 1.037 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, 0.024 um; position -0.107, -0.881 Measured defocus = -3.96 microns drift = 0.26 nm/sec Tracking image shifted 0.009, -0.002 um; position -0.116, -0.879 8 summed frames were saved to D:\RawFrames\DE20\20170914_99670_tomo2_020_-38p7 Record image shifted 0.010, -0.060 um Tracking reference shifted by 64.2,10.6 pixels, 0.010,-0.060 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 20 at 11.59 min Tilt = -38.66 Mean = 2317 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.126 -0.819 1.003 Errors in X/Y Prediction -0.018 0.038 um, 2.4 5.2 % Error in Z Prediction -0.033 Predicted X = -0.132 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.868 StdErr = 0.040 nFit = 4 nDrop = 0 Predicted Z = 0.967 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.046 um; position -0.126, -0.914 8 summed frames were saved to D:\RawFrames\DE20\20170914_99671_tomo2_021_-41p0 Record image shifted 0.016, -0.059 um Tracking reference shifted by 63.3,17.5 pixels, 0.016,-0.059 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 21 at 12.10 min Tilt = -41.00 Mean = 2311 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.142 -0.855 0.967 Errors in X/Y Prediction -0.010 0.013 um, 1.4 1.8 % Predicted X = -0.163 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.861 StdErr = 0.022 nFit = 4 nDrop = 0 Predicted Z = 0.930 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.020, 0.084 um; position -0.143, -0.945 8 summed frames were saved to D:\RawFrames\DE20\20170914_99672_tomo2_022_-43p3 Record image shifted 0.015, -0.078 um Tracking reference shifted by 82.9,16.3 pixels, 0.015,-0.078 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 22 at 12.61 min Tilt = -43.26 Mean = 2305 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.158 -0.867 0.930 Measured defocus = -3.80 microns drift = 1.41 nm/sec Errors in X/Y Prediction 0.004 -0.006 um, 0.6 0.8 % Need regular tracking - Need autofocus Tracking image shifted -0.144, 0.053 um; position 0.007, 0.845 Measured defocus = -4.15 microns drift = 1.55 nm/sec Tracking image shifted -0.298, 0.141 um; position 0.305, 0.704 8 summed frames were saved to D:\RawFrames\DE20\20170914_99674_tomo2_023_20p9 Record image shifted 0.380, -0.158 um Tracking reference shifted by 169.8,405.3 pixels, 0.380,-0.158 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.325 in X, 0.182 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99675_tomo2_024_20p9 Record image shifted -0.000, 0.008 um Saved Z = 23 at 14.69 min Tilt = 20.85 Mean = 2322 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.075 0.854 0.929 Need regular tracking - Need autofocus Tracking image shifted -0.013, -0.064 um; position -0.061, 0.918 Measured defocus = -3.78 microns drift = 1.48 nm/sec Tracking image shifted -0.004, 0.083 um; position -0.057, 0.836 8 summed frames were saved to D:\RawFrames\DE20\20170914_99676_tomo2_025_23p7 Record image shifted -0.009, 0.071 um Tracking reference shifted by -75.5,-9.5 pixels, -0.009,0.071 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 24 at 15.47 min Tilt = 23.66 Mean = 2312 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.048 0.765 0.744 Predicted X = -0.021 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.676 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 0.559 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, -0.130 um; position -0.039, 0.805 Measured defocus = -4.04 microns drift = 2.29 nm/sec Tracking image shifted -0.001, 0.034 um; position -0.038, 0.771 8 summed frames were saved to D:\RawFrames\DE20\20170914_99677_tomo2_026_26p4 Record image shifted -0.009, 0.132 um Tracking reference shifted by -141.5,-9.6 pixels, -0.009,0.132 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.008 in X, 0.152 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99678_tomo2_027_26p4 Record image shifted -0.002, 0.040 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 25 at 16.89 min Tilt = 26.40 Mean = 2306 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.027 0.599 0.613 Errors in X/Y Prediction -0.006 -0.076 um, 0.8 10.3 % Error in Z Prediction 0.054 Predicted X = -0.006 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.485 StdErr = 0.057 nFit = 3 nDrop = 0 Predicted Z = 0.445 StdErr = 0.040 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.013, -0.152 um; position -0.019, 0.637 8 summed frames were saved to D:\RawFrames\DE20\20170914_99679_tomo2_028_29p1 Record image shifted 0.023, 0.215 um Tracking reference shifted by -230.0,24.8 pixels, 0.023,0.215 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.020 in X, 0.247 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99680_tomo2_029_29p1 Record image shifted 0.023, 0.056 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 26 at 17.79 min Tilt = 29.09 Mean = 2304 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.065 0.365 0.445 Errors in X/Y Prediction -0.059 -0.120 um, 7.9 16.1 % Predicted X = -0.102 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.176 StdErr = 0.051 nFit = 3 nDrop = 0 Predicted Z = 0.287 StdErr = 0.053 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.042, -0.263 um; position -0.060, 0.439 8 summed frames were saved to D:\RawFrames\DE20\20170914_99682_tomo2_030_31p7 Record image shifted 0.016, 0.121 um Tracking reference shifted by -129.2,17.1 pixels, 0.016,0.121 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 27 at 18.32 min Tilt = 31.71 Mean = 2289 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = -0.076 0.318 0.287 Errors in X/Y Prediction 0.027 0.142 um, 3.6 19.1 % Predicted X = -0.104 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.146 StdErr = 0.140 nFit = 3 nDrop = 0 Predicted Z = 0.129 StdErr = 0.067 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.033, -0.205 um; position -0.072, 0.351 Measured defocus = -5.35 microns drift = 4.44 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.16 microns drift = 16.59 nm/sec Tracking image shifted 0.351, 0.075 um; position -0.423, 0.276 8 summed frames were saved to D:\RawFrames\DE20\20170914_99683_tomo2_031_34p3 Record image shifted -0.265, 0.545 um Tracking reference shifted by -582.4,-282.6 pixels, -0.265,0.545 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.227 in X, 0.623 in Y) 2017/09/14 12:31 D:\serialem\hwei\091417\tomo2.st 0 28 3712 4984 1036 18.0 31.7 3.00 COS 18.3 1x1 DE20 19000 1 0.23 LD 0.0 0.0 Total dose in electrons/square Angstrom: Recording: 81.6 Tracking: 311.4 Focusing: 245.0 Tasks: 22.47 Previews: 0.4 Measured defocus = -3.74 microns drift = 1.10 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.015, 0.056 um; position 0.168, 0.341 Measured defocus = -4.10 microns drift = 0.74 nm/sec 8 summed frames were saved to D:\RawFrames\DE20\20170914_99686_tomo3_000_18p0 Saved Z = 0 at 0.00 min Tilt = 18.00 Mean = 2313 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.168 0.341 1.534 Need regular tracking - Need autofocus Tracking image shifted 0.036, 0.147 um; position 0.132, 0.195 Measured defocus = -4.03 microns drift = 0.16 nm/sec Tracking image shifted 0.003, -0.011 um; position 0.130, 0.206 8 summed frames were saved to D:\RawFrames\DE20\20170914_99687_tomo3_001_15p1 Record image shifted 0.000, 0.008 um Tracking reference shifted by -8.8,0.5 pixels, 0.000,0.008 um Saved Z = 1 at 0.77 min Tilt = 15.15 Mean = 2321 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.129 0.198 1.563 Predicted X = 0.091 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.054 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.591 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.018, -0.021 um; position 0.109, 0.075 Measured defocus = -3.98 microns drift = 0.11 nm/sec Tracking image shifted -0.000, -0.004 um; position 0.109, 0.079 8 summed frames were saved to D:\RawFrames\DE20\20170914_99688_tomo3_002_12p3 Record image shifted 0.000, 0.001 um Tracking reference shifted by -0.7,0.3 pixels, 0.000,0.001 um Saved Z = 2 at 1.53 min Tilt = 12.25 Mean = 2325 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.109 0.078 1.567 Errors in X/Y Prediction 0.018 0.025 um, 2.4 3.3 % Error in Z Prediction -0.024 Predicted X = 0.088 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.057 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Z = 1.588 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, -0.026 um; position 0.097, -0.032 8 summed frames were saved to D:\RawFrames\DE20\20170914_99689_tomo3_003_9p3 Record image shifted 0.001, -0.002 um Tracking reference shifted by 2.2,1.5 pixels, 0.001,-0.002 um Saved Z = 3 at 2.00 min Tilt = 9.32 Mean = 2327 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.095 -0.030 1.588 Errors in X/Y Prediction 0.007 0.028 um, 0.9 3.8 % Predicted X = 0.081 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.145 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Z = 1.604 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, -0.019 um; position 0.088, -0.127 Measured defocus = -3.99 microns drift = 0.23 nm/sec Tracking image shifted 0.000, -0.007 um; position 0.088, -0.120 8 summed frames were saved to D:\RawFrames\DE20\20170914_99690_tomo3_004_6p4 Record image shifted 0.001, -0.002 um Tracking reference shifted by 2.4,1.4 pixels, 0.001,-0.002 um Saved Z = 4 at 2.75 min Tilt = 6.36 Mean = 2331 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.087 -0.118 1.590 Errors in X/Y Prediction 0.005 0.027 um, 0.7 3.7 % Error in Z Prediction -0.014 Predicted X = 0.078 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.219 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Z = 1.598 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.009 um; position 0.085, -0.210 8 summed frames were saved to D:\RawFrames\DE20\20170914_99691_tomo3_005_3p4 Record image shifted -0.001, -0.002 um Tracking reference shifted by 2.6,-1.4 pixels, -0.001,-0.002 um Saved Z = 5 at 3.22 min Tilt = 3.38 Mean = 2329 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.086 -0.208 1.598 Errors in X/Y Prediction 0.008 0.011 um, 1.1 1.5 % Predicted X = 0.085 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.283 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Z = 1.607 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.014, 0.030 um; position 0.099, -0.313 8 summed frames were saved to D:\RawFrames\DE20\20170914_99692_tomo3_006_0p4 Record image shifted -0.000, -0.002 um Tracking reference shifted by 2.4,-0.4 pixels, -0.000,-0.002 um Saved Z = 6 at 3.69 min Tilt = 0.38 Mean = 2329 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.100 -0.311 1.607 Errors in X/Y Prediction 0.014 -0.028 um, 1.9 3.7 % Predicted X = 0.114 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.419 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Z = 1.617 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.016, -0.010 um; position 0.129, -0.408 Measured defocus = -3.92 microns drift = 0.27 nm/sec Tracking image shifted -0.002, -0.009 um; position 0.131, -0.399 8 summed frames were saved to D:\RawFrames\DE20\20170914_99693_tomo3_007_-2p6 Record image shifted 0.000, -0.001 um Tracking reference shifted by 1.0,0.3 pixels, 0.000,-0.001 um Saved Z = 7 at 4.64 min Tilt = -2.62 Mean = 2324 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.131 -0.398 1.532 Errors in X/Y Prediction 0.017 0.020 um, 2.3 2.8 % Error in Z Prediction -0.084 Predicted X = 0.162 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.492 StdErr = 0.019 nFit = 4 nDrop = 0 Predicted Z = 1.534 StdErr = 0.046 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.016 um; position 0.158, -0.508 8 summed frames were saved to D:\RawFrames\DE20\20170914_99694_tomo3_008_-5p6 Record image shifted 0.002, -0.002 um Tracking reference shifted by 2.2,1.8 pixels, 0.002,-0.002 um Saved Z = 8 at 5.94 min Tilt = -5.61 Mean = 2323 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.156 -0.506 1.534 Errors in X/Y Prediction -0.006 -0.014 um, 0.8 1.9 % Predicted X = 0.181 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.607 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Z = 1.526 StdErr = 0.052 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.023, 0.013 um; position 0.158, -0.621 8 summed frames were saved to D:\RawFrames\DE20\20170914_99695_tomo3_009_-8p6 Record image shifted 0.000, 0.005 um Tracking reference shifted by -5.5,0.5 pixels, 0.000,0.005 um Saved Z = 9 at 6.61 min Tilt = -8.60 Mean = 2326 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.158 -0.626 1.526 Errors in X/Y Prediction -0.024 -0.019 um, 3.2 2.5 % Predicted X = 0.159 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.764 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Z = 1.518 StdErr = 0.058 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.034 um; position 0.156, -0.730 8 summed frames were saved to D:\RawFrames\DE20\20170914_99696_tomo3_010_-11p6 Record image shifted 0.000, -0.003 um Tracking reference shifted by 3.0,0.5 pixels, 0.000,-0.003 um Saved Z = 10 at 7.09 min Tilt = -11.55 Mean = 2324 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.156 -0.728 1.518 Errors in X/Y Prediction -0.003 0.036 um, 0.4 4.9 % Predicted X = 0.154 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.834 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Z = 1.510 StdErr = 0.065 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.009 um; position 0.157, -0.824 Measured defocus = -4.07 microns drift = 0.42 nm/sec Tracking image shifted 0.001, -0.017 um; position 0.156, -0.808 8 summed frames were saved to D:\RawFrames\DE20\20170914_99697_tomo3_011_-14p5 Record image shifted 0.003, 0.005 um Tracking reference shifted by -4.9,3.5 pixels, 0.003,0.005 um Saved Z = 11 at 7.85 min Tilt = -14.50 Mean = 2318 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.153 -0.812 1.587 Errors in X/Y Prediction -0.002 0.021 um, 0.3 2.9 % Error in Z Prediction 0.076 Predicted X = 0.149 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.879 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Z = 1.571 StdErr = 0.068 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, 0.024 um; position 0.155, -0.903 8 summed frames were saved to D:\RawFrames\DE20\20170914_99698_tomo3_012_-17p4 Record image shifted 0.000, 0.004 um Tracking reference shifted by -4.7,0.4 pixels, 0.000,0.004 um Saved Z = 12 at 8.33 min Tilt = -17.40 Mean = 2315 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.154 -0.908 1.571 Errors in X/Y Prediction 0.005 -0.028 um, 0.7 3.8 % Predicted X = 0.156 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.993 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Z = 1.571 StdErr = 0.074 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.017 um; position 0.158, -1.010 8 summed frames were saved to D:\RawFrames\DE20\20170914_99699_tomo3_013_-20p3 Record image shifted -0.001, 0.006 um Tracking reference shifted by -6.7,-1.3 pixels, -0.001,0.006 um Saved Z = 13 at 8.80 min Tilt = -20.26 Mean = 2315 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.159 -1.016 1.571 Errors in X/Y Prediction 0.003 -0.023 um, 0.4 3.1 % Predicted X = 0.164 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.136 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Z = 1.572 StdErr = 0.081 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.013 um; position 0.162, -1.123 8 summed frames were saved to D:\RawFrames\DE20\20170914_99700_tomo3_014_-23p1 Record image shifted 0.001, -0.001 um Tracking reference shifted by 0.8,1.1 pixels, 0.001,-0.001 um Saved Z = 14 at 9.28 min Tilt = -23.08 Mean = 2309 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.161 -1.122 1.572 Errors in X/Y Prediction -0.003 0.014 um, 0.4 1.8 % Predicted X = 0.163 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.238 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Z = 1.572 StdErr = 0.088 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.023 um; position 0.168, -1.214 Measured defocus = -3.93 microns drift = 0.10 nm/sec Tracking image shifted 0.000, -0.009 um; position 0.168, -1.205 8 summed frames were saved to D:\RawFrames\DE20\20170914_99701_tomo3_015_-25p8 Record image shifted 0.000, 0.007 um Tracking reference shifted by -7.0,0.4 pixels, 0.000,0.007 um Saved Z = 15 at 10.03 min Tilt = -25.83 Mean = 2314 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.167 -1.212 1.503 Errors in X/Y Prediction 0.004 0.026 um, 0.6 3.5 % Error in Z Prediction -0.069 Predicted X = 0.174 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.295 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Z = 1.522 StdErr = 0.082 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.004 um; position 0.176, -1.299 8 summed frames were saved to D:\RawFrames\DE20\20170914_99702_tomo3_016_-28p5 Record image shifted -0.002, 0.004 um Tracking reference shifted by -4.8,-2.5 pixels, -0.002,0.004 um Saved Z = 16 at 10.50 min Tilt = -28.53 Mean = 2301 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.179 -1.303 1.522 Errors in X/Y Prediction 0.005 -0.008 um, 0.7 1.1 % Predicted X = 0.190 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.383 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Z = 1.519 StdErr = 0.088 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, -0.007 um; position 0.199, -1.376 8 summed frames were saved to D:\RawFrames\DE20\20170914_99703_tomo3_017_-31p2 Record image shifted -0.002, -0.004 um Tracking reference shifted by 4.3,-1.9 pixels, -0.002,-0.004 um Saved Z = 17 at 10.97 min Tilt = -31.17 Mean = 2293 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.201 -1.372 1.519 Errors in X/Y Prediction 0.011 0.011 um, 1.5 1.5 % Predicted X = 0.216 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -1.437 StdErr = 0.017 nFit = 4 nDrop = 0 Predicted Z = 1.515 StdErr = 0.095 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.013, -0.001 um; position 0.229, -1.436 8 summed frames were saved to D:\RawFrames\DE20\20170914_99704_tomo3_018_-33p7 Record image shifted -0.001, 0.007 um Tracking reference shifted by -7.1,-1.4 pixels, -0.001,0.007 um Saved Z = 18 at 11.44 min Tilt = -33.74 Mean = 2281 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.230 -1.442 1.515 Errors in X/Y Prediction 0.014 -0.005 um, 1.9 0.7 % Predicted X = 0.255 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.495 StdErr = 0.016 nFit = 4 nDrop = 0 Predicted Z = 1.511 StdErr = 0.103 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, 0.004 um; position 0.263, -1.499 Measured defocus = -3.79 microns drift = 0.48 nm/sec Tracking image shifted -0.009, -0.019 um; position 0.272, -1.480 8 summed frames were saved to D:\RawFrames\DE20\20170914_99705_tomo3_019_-36p2 Record image shifted 0.003, 0.016 um Tracking reference shifted by -17.5,3.5 pixels, 0.003,0.016 um Saved Z = 19 at 12.20 min Tilt = -36.23 Mean = 2283 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.269 -1.496 1.314 Errors in X/Y Prediction 0.014 -0.001 um, 1.9 0.1 % Error in Z Prediction -0.197 Predicted X = 0.301 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -1.548 StdErr = 0.011 nFit = 4 nDrop = 0 Predicted Z = 1.298 StdErr = 0.062 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.005, 0.019 um; position 0.296, -1.567 8 summed frames were saved to D:\RawFrames\DE20\20170914_99706_tomo3_020_-38p6 Record image shifted -0.003, -0.001 um Tracking reference shifted by 1.3,-2.9 pixels, -0.003,-0.001 um Saved Z = 20 at 12.67 min Tilt = -38.65 Mean = 2266 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.299 -1.565 1.298 Errors in X/Y Prediction -0.002 -0.018 um, 0.2 2.4 % Predicted X = 0.335 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -1.626 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Z = 1.264 StdErr = 0.067 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, -0.073 um; position 0.335, -1.553 8 summed frames were saved to D:\RawFrames\DE20\20170914_99707_tomo3_021_-41p0 Record image shifted -0.002, 0.001 um Tracking reference shifted by -1.1,-1.9 pixels, -0.002,0.001 um Saved Z = 21 at 13.14 min Tilt = -40.99 Mean = 2261 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.337 -1.554 1.264 Errors in X/Y Prediction 0.002 0.072 um, 0.2 9.7 % Predicted X = 0.369 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.533 StdErr = 0.063 nFit = 4 nDrop = 0 Predicted Z = 1.230 StdErr = 0.072 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, 0.059 um; position 0.381, -1.592 8 summed frames were saved to D:\RawFrames\DE20\20170914_99708_tomo3_022_-43p3 Record image shifted 0.000, 0.013 um Tracking reference shifted by -13.8,0.5 pixels, 0.000,0.013 um Saved Z = 22 at 13.61 min Tilt = -43.25 Mean = 2252 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.381 -1.605 1.230 Measured defocus = -4.08 microns drift = 1.32 nm/sec Errors in X/Y Prediction 0.011 -0.072 um, 1.5 9.7 % Need regular tracking - Need autofocus Tracking image shifted -0.039, -0.124 um; position -0.009, 1.139 Measured defocus = -3.93 microns drift = 1.37 nm/sec Tracking image shifted -0.017, 0.055 um; position 0.007, 1.085 8 summed frames were saved to D:\RawFrames\DE20\20170914_99709_tomo3_023_20p9 Record image shifted -0.493, 0.014 um Tracking reference shifted by -15.9,-526.9 pixels, -0.493,0.014 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.422 in X, 0.016 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99710_tomo3_024_20p9 Record image shifted -0.017, 0.037 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 23 at 15.67 min Tilt = 20.85 Mean = 2311 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.517 1.034 1.265 Need regular tracking - Need autofocus Tracking image shifted -0.015, -0.081 um; position 0.532, 1.114 Measured defocus = -3.93 microns drift = 1.58 nm/sec Tracking image shifted -0.000, 0.082 um; position 0.532, 1.032 8 summed frames were saved to D:\RawFrames\DE20\20170914_99711_tomo3_025_23p7 Record image shifted -0.006, 0.042 um Tracking reference shifted by -45.4,-6.8 pixels, -0.006,0.042 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 24 at 16.45 min Tilt = 23.66 Mean = 2316 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.539 0.990 1.229 Predicted X = 0.560 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.946 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.193 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, -0.113 um; position 0.550, 1.059 Measured defocus = -4.44 microns drift = 9.77 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -3.50 microns drift = 1.20 nm/sec Tracking image shifted -0.013, 0.055 um; position 0.563, 1.004 8 summed frames were saved to D:\RawFrames\DE20\20170914_99712_tomo3_026_26p4 Record image shifted -0.005, 0.038 um Tracking reference shifted by -40.5,-5.2 pixels, -0.005,0.038 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 25 at 17.32 min Tilt = 26.40 Mean = 2308 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.568 0.966 1.157 Errors in X/Y Prediction 0.008 0.021 um, 1.1 2.8 % Error in Z Prediction -0.036 Predicted X = 0.598 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.929 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Z = 1.109 StdErr = 0.027 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.019, -0.079 um; position 0.579, 1.008 8 summed frames were saved to D:\RawFrames\DE20\20170914_99713_tomo3_027_29p1 Record image shifted 0.017, 0.388 um Tracking reference shifted by -414.3,19.0 pixels, 0.017,0.388 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.015 in X, 0.446 in Y) Redoing Record because position is too far off 8 summed frames were saved to D:\RawFrames\DE20\20170914_99714_tomo3_028_29p1 Record image shifted -0.005, -0.002 um Saved Z = 26 at 17.91 min Tilt = 29.09 Mean = 2307 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.566 0.622 1.109 Errors in X/Y Prediction -0.032 -0.307 um, 4.3 41.4 % Predicted X = 0.564 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.492 StdErr = 0.239 nFit = 3 nDrop = 0 Predicted Z = 1.055 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.018, -0.225 um; position 0.582, 0.717 8 summed frames were saved to D:\RawFrames\DE20\20170914_99715_tomo3_029_31p7 Record image shifted -0.035, 0.028 um Tracking reference shifted by -29.5,-37.0 pixels, -0.035,0.028 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 27 at 18.42 min Tilt = 31.71 Mean = 2303 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.616 0.689 1.055 Errors in X/Y Prediction 0.052 0.197 um, 7.1 26.6 % Predicted X = 0.631 StdErr = 0.039 nFit = 3 nDrop = 0 Predicted Y = 0.482 StdErr = 0.307 nFit = 3 nDrop = 0 Predicted Z = 1.001 StdErr = 0.045 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.297 um; position 0.634, 0.779 Measured defocus = -4.18 microns drift = 0.61 nm/sec Tracking image shifted -0.019, 0.018 um; position 0.653, 0.760 8 summed frames were saved to D:\RawFrames\DE20\20170914_99716_tomo3_030_34p3 Record image shifted -0.020, 0.021 um Tracking reference shifted by -22.4,-20.9 pixels, -0.020,0.021 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 28 at 19.21 min Tilt = 34.26 Mean = 2289 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.672 0.739 1.192 Errors in X/Y Prediction 0.041 0.258 um, 5.5 34.7 % Error in Z Prediction 0.191 Predicted X = 0.724 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 1.094 StdErr = 0.283 nFit = 4 nDrop = 0 Predicted Z = 1.178 StdErr = 0.080 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.034, 0.281 um; position 0.691, 0.814 8 summed frames were saved to D:\RawFrames\DE20\20170914_99717_tomo3_031_36p7 Record image shifted -0.017, 0.053 um Tracking reference shifted by -56.1,-18.6 pixels, -0.017,0.053 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 29 at 19.71 min Tilt = 36.74 Mean = 2281 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.708 0.761 1.178 Errors in X/Y Prediction -0.016 -0.333 um, 2.2 44.9 % Predicted X = 0.757 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.763 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Z = 1.174 StdErr = 0.092 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.034, -0.050 um; position 0.724, 0.813 8 summed frames were saved to D:\RawFrames\DE20\20170914_99718_tomo3_032_39p1 Record image shifted -0.021, 0.107 um Tracking reference shifted by -114.9,-22.2 pixels, -0.021,0.107 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 30 at 20.21 min Tilt = 39.14 Mean = 2269 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.745 0.706 1.174 Errors in X/Y Prediction -0.013 -0.057 um, 1.7 7.7 % Predicted X = 0.781 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.610 StdErr = 0.032 nFit = 4 nDrop = 0 Predicted Z = 1.170 StdErr = 0.106 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.022, -0.128 um; position 0.759, 0.738 Measured defocus = -3.98 microns drift = 6.43 nm/sec Tracking image shifted 0.003, 0.378 um; position 0.756, 0.360 8 summed frames were saved to D:\RawFrames\DE20\20170914_99719_tomo3_033_41p5 Record image shifted -0.030, 0.043 um Tracking reference shifted by -45.9,-31.8 pixels, -0.030,0.043 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 31 at 20.99 min Tilt = 41.47 Mean = 2259 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.786 0.318 1.480 Errors in X/Y Prediction 0.005 -0.292 um, 0.6 39.4 % Error in Z Prediction 0.310 Predicted X = 0.824 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.212 StdErr = 0.169 nFit = 4 nDrop = 0 Predicted Z = 1.492 StdErr = 0.154 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.037, -0.441 um; position 0.861, 0.230 Measured defocus = -3.57 microns drift = 0.73 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.08 microns drift = 0.66 nm/sec Tracking image shifted 0.028, -0.144 um; position 0.833, 0.374 8 summed frames were saved to D:\RawFrames\DE20\20170914_99720_tomo3_034_43p7 Record image shifted -0.028, 0.025 um Tracking reference shifted by -26.6,-29.4 pixels, -0.028,0.025 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 32 at 21.86 min Tilt = 43.72 Mean = 2253 C2 = 51.28% dose = 2.92 e/A2 X/Y/Z = 0.860 0.349 0.999 2017/09/14 13:05 D:\serialem\hwei\091417\tomo3.st 0 33 3712 4984 1221 18.0 43.7 3.00 COS 21.9 1x1 DE20 19000 1 0.23 LD 0.0 0.0 Total dose in electrons/square Angstrom: Recording: 96.2 Tracking: 301.2 Focusing: 275.7 Tasks: 22.47 Previews: 0.4