DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0011_thick300/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0011_thick300/defocus_estimation/series0011_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0011_thick300/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 44000.0, 66000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -45.0 64500.0 44000.0 65500.0 1 -42.5 66000.0 52500.0 54500.0 2 -40.0 53500.0 66000.0 44000.0 3 -37.5 58000.0 65000.0 58000.0 4 -35.0 60000.0 62500.0 66000.0 5 -32.5 59500.0 61500.0 60500.0 6 -30.0 59500.0 57000.0 65000.0 7 -27.5 59000.0 66000.0 62000.0 8 -25.0 62500.0 62000.0 61500.0 9 -22.5 58500.0 59500.0 61500.0 10 -20.0 54000.0 59000.0 51000.0 11 -17.5 56500.0 58000.0 61000.0 12 -15.0 55000.0 57500.0 57500.0 13 -12.5 54000.0 48000.0 57500.0 14 -10.0 49000.0 57500.0 44000.0 15 -7.5 56000.0 57000.0 59500.0 16 -5.0 44500.0 62000.0 58500.0 17 -2.5 54000.0 56000.0 58000.0 18 -0.1 54000.0 62000.0 56000.0 19 0.0 55000.0 58000.0 59500.0 20 2.5 53500.0 53500.0 54000.0 21 5.0 52000.0 53000.0 56500.0 22 7.5 55000.0 53500.0 53000.0 23 10.0 47000.0 48500.0 49000.0 24 12.5 51000.0 51000.0 51500.0 25 15.0 50000.0 53000.0 53000.0 26 17.5 50500.0 50500.0 48500.0 27 20.0 49000.0 49000.0 47500.0 28 22.5 51500.0 47500.0 50500.0 29 25.0 48500.0 45000.0 46000.0 30 27.5 47500.0 47500.0 52500.0 31 30.0 45000.0 46000.0 47000.0 32 32.5 49000.0 47500.0 46000.0 33 35.0 47000.0 48500.0 46000.0 34 37.5 48500.0 50000.0 44000.0 35 40.0 48000.0 49000.0 44000.0 36 42.5 47000.0 46000.0 44500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)