DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0013_thick400/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0013_thick400/defocus_estimation/series0013_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/17jul12a/protomo_alignments/tiltseries0013_thick400/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 48000.0, 72000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 45.0 49000.0 51500.0 51000.0 1 42.5 67500.0 48500.0 71500.0 2 40.0 53000.0 71000.0 50500.0 3 37.5 61500.0 68500.0 51000.0 4 35.0 56000.0 71500.0 53000.0 5 32.5 65000.0 66500.0 70500.0 6 30.0 48500.0 70500.0 70500.0 7 27.5 66500.0 66000.0 50000.0 8 25.0 69000.0 57000.0 66500.0 9 22.5 56000.0 61000.0 67000.0 10 20.0 61000.0 69000.0 63500.0 11 17.5 64000.0 68500.0 62500.0 12 15.0 59000.0 55000.0 64500.0 13 12.5 65000.0 62000.0 57500.0 14 10.0 68500.0 61500.0 53500.0 15 7.5 71000.0 48000.0 62500.0 16 5.0 63500.0 59500.0 61000.0 17 2.5 59000.0 66500.0 71000.0 18 0.1 51000.0 54000.0 57500.0 19 -0.0 53000.0 60500.0 56500.0 20 -2.5 60000.0 49500.0 55500.0 21 -5.0 59000.0 48000.0 49000.0 22 -7.5 57500.0 57500.0 56500.0 23 -10.0 55000.0 59000.0 49500.0 24 -12.5 54500.0 54000.0 50500.0 25 -15.0 57000.0 53500.0 62500.0 26 -17.5 48500.0 49500.0 58000.0 27 -20.0 54000.0 61000.0 58000.0 28 -22.5 54000.0 65000.0 53500.0 29 -25.0 50500.0 50000.0 48000.0 30 -27.5 60000.0 60500.0 48500.0 31 -30.0 57500.0 52500.0 61000.0 32 -32.5 55000.0 49500.0 64500.0 33 -35.0 49500.0 48000.0 48500.0 34 -37.5 49500.0 68500.0 48000.0 35 -40.0 53500.0 51500.0 58000.0 36 -42.5 49500.0 49500.0 48000.0 37 -45.0 48000.0 48000.0 52000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)