DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation/series0047_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 2.4, 3.60, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -52.0 0.0 0.0 0.0 1 -50.0 0.0 0.0 0.0 2 -48.0 0.0 0.0 0.0 3 -46.0 0.0 0.0 0.0 4 -44.0 0.0 0.0 0.0 5 -42.0 0.0 0.0 0.0 6 -40.0 0.0 0.0 0.0 7 -38.0 0.0 0.0 0.0 8 -36.0 0.0 0.0 0.0 9 -34.0 0.0 0.0 0.0 10 -32.0 0.0 0.0 0.0 11 -30.0 0.0 0.0 0.0 12 -28.0 0.0 0.0 0.0 13 -26.0 0.0 0.0 0.0 14 -24.0 0.0 0.0 0.0 15 -22.0 0.0 0.0 0.0 16 -20.0 0.0 0.0 0.0 17 -18.0 0.0 0.0 0.0 18 -16.0 0.0 0.0 0.0 19 -14.0 0.0 0.0 0.0 20 -12.0 0.0 0.0 0.0 21 -10.0 0.0 0.0 0.0 22 -8.0 0.0 0.0 0.0 23 -6.0 0.0 0.0 0.0 24 -4.0 0.0 0.0 0.0 25 -2.0 0.0 0.0 0.0 26 -0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 28 2.0 0.0 0.0 0.0 29 4.0 0.0 0.0 0.0 30 6.0 0.0 0.0 0.0 31 8.0 0.0 0.0 0.0 32 10.0 0.0 0.0 0.0 33 12.0 0.0 0.0 0.0 34 14.0 0.0 0.0 0.0 35 16.0 0.0 0.0 0.0 36 18.0 0.0 0.0 0.0 37 20.0 0.0 0.0 0.0 38 22.0 0.0 0.0 0.0 39 24.0 0.0 0.0 0.0 40 26.0 0.0 0.0 0.0 41 28.0 0.0 0.0 0.0 42 30.0 0.0 0.0 0.0 43 32.0 0.0 0.0 0.0 44 34.0 0.0 0.0 0.0 45 36.0 0.0 0.0 0.0 46 38.0 0.0 0.0 0.0 47 40.0 0.0 0.0 0.0 48 42.0 0.0 0.0 0.0 49 44.0 0.0 0.0 0.0 50 46.0 0.0 0.0 0.0 51 48.0 0.0 0.0 0.0 52 50.0 0.0 0.0 0.0 53 52.0 0.0 0.0 0.0 54 54.0 0.0 0.0 0.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)