DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation/series0047_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/17feb10h/protomo_alignments/tiltseries0047dc/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 24000.0, 36000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 52.0 35000.0 35000.0 25500.0 1 50.0 27000.0 25000.0 25000.0 2 48.0 25500.0 30500.0 28000.0 3 46.0 32000.0 24000.0 28000.0 4 44.0 24500.0 24000.0 30000.0 5 42.0 25000.0 31500.0 26500.0 6 40.0 24000.0 30500.0 27000.0 7 38.0 24000.0 28000.0 28000.0 8 36.0 30500.0 24000.0 33500.0 9 34.0 24000.0 31000.0 28000.0 10 32.0 28500.0 29500.0 30000.0 11 30.0 27000.0 30000.0 36000.0 12 28.0 24500.0 32500.0 27500.0 13 26.0 31500.0 32000.0 32000.0 14 24.0 32500.0 25000.0 35500.0 15 22.0 24000.0 28000.0 33500.0 16 20.0 26500.0 27000.0 27000.0 17 18.0 25000.0 34000.0 32000.0 18 16.0 26000.0 27500.0 33500.0 19 14.0 30500.0 30500.0 26500.0 20 12.0 24500.0 24000.0 36000.0 21 10.0 31000.0 26000.0 30500.0 22 8.0 26000.0 27500.0 30500.0 23 6.0 34500.0 26000.0 29500.0 24 4.0 25500.0 25500.0 26000.0 25 2.0 30500.0 30000.0 25500.0 26 0.0 29000.0 32500.0 29000.0 27 -0.0 24000.0 28000.0 24500.0 28 -2.0 24000.0 24500.0 24000.0 29 -4.0 28000.0 30000.0 34000.0 30 -6.0 24500.0 32000.0 26500.0 31 -8.0 29500.0 30500.0 29500.0 32 -10.0 35000.0 29000.0 24000.0 33 -12.0 28500.0 24000.0 24500.0 34 -14.0 27500.0 26500.0 27000.0 35 -16.0 26000.0 36000.0 29500.0 36 -18.0 30000.0 28000.0 27000.0 37 -20.0 26000.0 27500.0 26000.0 38 -22.0 29500.0 30000.0 30500.0 39 -24.0 29000.0 29500.0 24000.0 40 -26.0 26000.0 28000.0 31000.0 41 -28.0 24500.0 30000.0 26500.0 42 -30.0 24000.0 29500.0 29500.0 43 -32.0 26500.0 24500.0 27000.0 44 -34.0 29000.0 25000.0 29000.0 45 -36.0 31000.0 24000.0 24000.0 46 -38.0 30000.0 24000.0 25000.0 47 -40.0 29500.0 24500.0 28000.0 48 -42.0 28000.0 24000.0 29000.0 49 -44.0 27000.0 28000.0 24000.0 50 -46.0 30000.0 24000.0 25000.0 51 -48.0 26000.0 28500.0 24500.0 52 -50.0 30500.0 24500.0 24000.0 53 -52.0 27000.0 33500.0 29500.0 54 -54.0 34500.0 31000.0 24000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)