REL 2019-05-17 2020-03-02 2020-03-02 0000-0002-5457-4478 Beata Turonova Structural and Computational Biology, European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg Germany 69117 0000-0002-7397-1321 Martin Beck Structural and Computational Biology, European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg Germany 69117 Turoňová B Hagen WJH Obr M Mosalaganti S Beugelink JW Zimmerli CE Kräusslich HG Beck M 45.6 10.6019/EMPIAR-10277 EMDB EMD-10207 Turoňová B Hagen WJH Obr M Mosalaganti S Beugelink JW Zimmerli CE Kräusslich HG Beck M Nature communications Nat Commun 1 11 2020 English 10.1038/s41467-020-14535-2 32054835 Unsorted raw tilt-series for benchmarked tilt-schemes /data/tilt_series tilt series MRC MRC 35 41 SIGNED 16 BIT INTEGER 3708 1.3269 3838 1.3269

Raw, unsorted, non-dose-filtered tilt-series for 7 different tilt-schemes (5 tilt-series per each tilt-scheme). Each tilt-series contains 41 images - each image is an motion-corrected average of 10-20 frames.

Tilt-series collected using the dose-symmetric scheme with 3 degrees tilt-step without any gold fiducials /data/no_fiducials tilt series MRC MRC 8 41 SIGNED 16 BIT INTEGER variable 1.3269 3838 1.3269

Tilt-series acquired using the dose-symmetric tilt-scheme with 3 degrees tilt-step (tilt-range from -60 to +60 degrees) without any gold fiducials. The tilt-series are either not complete (i.e. have less than 41 images) and/or are cut in one dimension to remove any fiducials from the field of view. The trimming was done on the frame level to ensure that the final motion-corrected images do not profit from the presence of any fiducials. The tilt angles are contained in the header. The axis angle for all tilt-series was 85.5 degrees.