REL 2019-10-23 2020-01-31 2020-04-24 0000-0003-1909-5751 Melody Grace Campbell UCSF 600 16th Street, Room S474 San Francisco CA United States 94143 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street, Room S472D San Francisco CA United States 94143 Campbell MG Cheng Y 1.2 10.6019/EMPIAR-10343 EMDB EMD-20794 EMD-20797 EMD-20798 EMD-20799 EMD-20800 EMD-20801 EMD-20802 EMD-21125 Campbell MG Cormier A Ito S Seed RI Bondesson AJ Lou J Marks JD Baron J Cheng Y Nishimura SL Cell Cell 2020 English 10.1016/j.cell.2019.12.030 31955848 Unaligned 80-frame movies of αVβ8 integrin bound to latent TGF-β on a holey carbon grid /data/Movies_18aug24b micrographs - multiframe TIFF TIFF 1684 80 UNSIGNED BYTE 7676 0.6725 7420 0.6725

αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): 1684 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 16 second movies contain 80 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~70 e/Å^2. The gain reference, a file containing coordinates for a single dead column, and a list of the 1364 micrographs that were used for downstream processing are included.

data/Movies_18aug24b/18aug24b_*.tif data/Stack_18aug24b/Micrographs/18aug24b_*_DW.mrcs data/Stack_18aug24b/Micrographs Dose-weighted aligned micrographs of αVβ8 integrin bound to latent TGF-β on a holey carbon grid /data/Micrographs_18aug24b micrographs - single frame MRC MRC 1684 1 32 BIT FLOAT 3838 1.345 3710 1.345

αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): 1684 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~70 e/Å^2. A list of the 1364 micrographs that were used for downstream processing is included.

data/Micrographs_18aug24b/18aug24b_*.mrc data/Stack_18aug24b/Micrographs/18aug24b_*_DW.mrcs data/Stack_18aug24b/Micrographs Dose-weighted aligned particle stacks of αVβ8 integrin bound to latent TGF-β on a holey carbon grid /data/Stack_18aug24b picked particles - single frame - processed MRCS MRCS 68356 1 32 BIT FLOAT 300 1.345 300 1.345

αVβ8 integrin bound to latent TGF-β on holey carbon grids (particles have strongly preferred orientations): Stacks of 68,356 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after extensive 2D- and 3D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. This particle stack was combined with a stack of particles collected on graphene oxide to complement preferred orientations. The graphene oxide particle stack is deposited with the accession number EMPIAR-10344.

data/Micrographs_18aug24b/18aug24b_*.mrc data/Stack_18aug24b/Micrographs/18aug24b_*_DW.mrcs data/Stack_18aug24b/Micrographs