- The data were collected in two cryo-EM sessions. The movies are in separate folders according to the microscope session: 20181213_PS2.3.3 and PS.TT.3
- The run_data.star file contains particle information from the final 3D refinement in Relion.

Details for the 20181213_PS2.3.3 session:

- Approximately two-thirds of the data were collected with the Volta phase plate (VPP). "VPP" in the filename indicates that the movie was collected with the phase plate.
- Most movies were collected with a 9-hole beam-image shift acquisition pattern. The filename of such movies contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement. 
- The movies were recorded with Gatan K3 camera in super-resolution mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- SuperRes pixel size: 0.413 A/superpixel
- Physical pixel size: 0.826 A/physpix
- Exposure rate: 10.9 e/physpix/s
- Exposure rate: 16.0 e/A^2/s
- Exposure time: 4.015 s
- Total exposure: 64.2 e/A^2
- Number of frames: 67
- Defocus range: 0.3 - 1.4 um



Details for the PS.TT.3 session:

- The movies were recorded with Gatan K3 camera in counting (non-superres) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- All movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement. 
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Pixel size: 0.826 A/pix
- Exposure rate: 12.2 e/physpix/s
- Exposure rate: 17.9 e/A^2/s
- Exposure time: 3.715 s
- Total exposure: 66.5 e/A^2
- Number of frames: 62
- Defocus range: 0.6 - 1.5 um