Experimental details: - Approximately half of the data were collected with the Volta phase plate (VPP). Such movies are in the "VPP" subfolder and contain "VPP" in their filename. - The movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position. - The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift-position beam-tilt measurement and correction with CTF refinement. - The movies were recorded with Gatan K3 camera in counting (hardware bin by 2) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM. - Accelerating voltage: 300 kV - Spherical aberration: 2.7 mm - Pixel size: 0.826 A/pix - Exposure rate: 11.75 e/pix/s - Exposure rate: 17.22 e/A^2/s - Exposure time: 3.715 s - Total exposure: 64.0 e/A^2 - Number of frames: 62 - Defocus range: 0.4 - 1.6 um