DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0002/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0002/defocus_estimation/series0002_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0002/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32817.7, 49226.61, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -60.6 45500.0 44500.0 35500.0 1 -58.3 49000.0 42500.0 38500.0 2 -56.0 36000.0 34000.0 34000.0 3 -53.7 33000.0 44500.0 42500.0 4 -51.4 33000.0 40000.0 36500.0 5 -49.1 34500.0 47500.0 36000.0 6 -46.8 46500.0 42500.0 39500.0 7 -44.5 33500.0 39000.0 39000.0 8 -42.2 42500.0 44000.0 36000.0 9 -39.9 47000.0 40500.0 39000.0 10 -37.6 45000.0 44500.0 40000.0 11 -35.3 49000.0 44000.0 39500.0 12 -33.0 44000.0 38500.0 40500.0 13 -30.7 46500.0 46500.0 41000.0 14 -28.4 47500.0 43500.0 45500.0 15 -26.1 45500.0 46500.0 42500.0 16 -23.8 46000.0 40000.0 42500.0 17 -21.5 44500.0 46000.0 43500.0 18 -19.2 34000.0 46000.0 45000.0 19 -16.9 46500.0 45500.0 45500.0 20 -14.6 48000.0 48500.0 33000.0 21 -12.3 48500.0 49000.0 48000.0 22 -10.0 34500.0 48500.0 48000.0 23 -10.0 47000.0 46000.0 45000.0 24 -7.7 46500.0 47000.0 47500.0 25 -5.4 45000.0 45500.0 47500.0 26 -3.1 43500.0 44500.0 44000.0 27 -0.8 43000.0 42000.0 44000.0 28 1.5 44500.0 44000.0 46000.0 29 3.8 45000.0 45500.0 45000.0 30 6.1 45500.0 44500.0 44000.0 31 8.4 43500.0 44000.0 45000.0 32 10.7 43500.0 44500.0 42500.0 33 13.0 42000.0 45000.0 44500.0 34 15.3 40500.0 44500.0 47000.0 35 17.6 43000.0 44500.0 46500.0 36 19.9 42500.0 45500.0 43000.0 37 22.2 43500.0 44000.0 46500.0 38 24.5 39500.0 41500.0 42500.0 39 26.8 47500.0 41000.0 43000.0 40 29.1 38000.0 39500.0 44000.0 41 31.4 37000.0 40500.0 44500.0 42 33.7 40000.0 40000.0 46000.0 43 36.0 33500.0 42000.0 41500.0 44 38.3 38500.0 38500.0 41500.0 45 40.6 32500.0 38500.0 42500.0 46 42.9 33500.0 39500.0 41500.0 47 45.2 34000.0 38500.0 40500.0 48 47.5 49000.0 36500.0 39500.0 49 49.8 32500.0 37000.0 39500.0 50 52.1 36000.0 36000.0 38500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 118.416u 1.150s 1:59.84 99.7% 0+0k 0+0io 0pf+0w