DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0003/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0003/defocus_estimation/series0003_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0003/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32250.6, 48375.94, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -60.6 47000.0 42500.0 32000.0 1 -58.3 44000.0 42500.0 35000.0 2 -56.0 37000.0 41500.0 33500.0 3 -53.7 48000.0 37000.0 34500.0 4 -51.4 32000.0 34500.0 35500.0 5 -49.1 44000.0 37500.0 41500.0 6 -46.8 38500.0 39000.0 32000.0 7 -44.5 44000.0 48000.0 40500.0 8 -42.2 46000.0 41500.0 43000.0 9 -39.9 46500.0 42000.0 37500.0 10 -37.6 48000.0 43000.0 39000.0 11 -35.3 34000.0 34500.0 33000.0 12 -33.0 47500.0 35500.0 37500.0 13 -30.7 47000.0 44500.0 45000.0 14 -28.4 47500.0 45500.0 46500.0 15 -26.1 48000.0 45500.0 45000.0 16 -23.8 46500.0 43500.0 33000.0 17 -21.5 48000.0 43000.0 48000.0 18 -19.2 32000.0 46500.0 48000.0 19 -16.9 48000.0 45500.0 44000.0 20 -14.6 37000.0 48000.0 33500.0 21 -12.3 48000.0 37000.0 35500.0 22 -10.0 34500.0 48000.0 48000.0 23 -10.0 48000.0 47000.0 47500.0 24 -7.7 32000.0 45500.0 40500.0 25 -5.4 46500.0 46500.0 44000.0 26 -3.1 47000.0 45500.0 47000.0 27 -0.8 46500.0 47000.0 47500.0 28 1.5 48000.0 46000.0 34500.0 29 3.8 46500.0 45500.0 45500.0 30 6.1 47000.0 47000.0 44500.0 31 8.4 45500.0 44000.0 46000.0 32 10.7 46500.0 46500.0 47000.0 33 13.0 45500.0 48000.0 47000.0 34 15.3 44000.0 44500.0 45000.0 35 17.6 43500.0 46000.0 45500.0 36 19.9 42500.0 44500.0 41500.0 37 22.2 41500.0 44500.0 32500.0 38 24.5 48000.0 44000.0 34500.0 39 26.8 40500.0 43000.0 45000.0 40 29.1 39000.0 43000.0 48000.0 41 31.4 46500.0 39500.0 47500.0 42 33.7 38000.0 43500.0 40000.0 43 36.0 38500.0 40500.0 42500.0 44 38.3 36000.0 46500.0 32500.0 45 40.6 34500.0 39500.0 37000.0 46 42.9 37500.0 35000.0 36000.0 47 45.2 33500.0 36000.0 42500.0 48 47.5 32000.0 37500.0 44000.0 49 49.8 42500.0 43000.0 33500.0 50 52.1 38500.0 41500.0 43000.0 51 54.4 33000.0 33000.0 38000.0 52 56.7 40000.0 46500.0 36500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 129.854u 1.323s 2:21.62 92.6% 0+0k 0+0io 0pf+0w