DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0004/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0004/defocus_estimation/series0004_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0004/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32080.0, 48120.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 60.6 34000.0 38500.0 33000.0 1 58.3 32500.0 34500.0 43000.0 2 56.0 45000.0 38500.0 34000.0 3 53.7 47000.0 39000.0 46000.0 4 51.4 46000.0 33000.0 0.0 5 49.1 43000.0 32000.0 37000.0 6 46.8 44500.0 44500.0 32000.0 7 44.5 46000.0 35500.0 40500.0 8 42.2 41500.0 43500.0 44500.0 9 39.9 44000.0 38500.0 39500.0 10 37.6 44500.0 43500.0 44500.0 11 35.3 43500.0 40500.0 34000.0 12 33.0 46500.0 44000.0 39500.0 13 30.7 46000.0 43500.0 36500.0 14 28.4 47500.0 45000.0 44500.0 15 26.1 46000.0 44500.0 40500.0 16 23.8 45500.0 48000.0 45000.0 17 21.5 47500.0 48000.0 45000.0 18 19.2 48000.0 45000.0 43000.0 19 16.9 32000.0 45000.0 48000.0 20 14.6 33000.0 46500.0 0.0 21 12.3 37000.0 45500.0 33000.0 22 10.0 48000.0 48000.0 46000.0 23 10.0 48000.0 48000.0 47500.0 24 7.7 33000.0 48000.0 48000.0 25 5.4 33000.0 48000.0 32000.0 26 3.1 47500.0 48000.0 48000.0 27 0.8 45000.0 47000.0 46000.0 28 -1.5 48000.0 48000.0 33000.0 29 -3.8 48000.0 46500.0 48000.0 30 -6.1 46500.0 46500.0 46000.0 31 -8.4 46000.0 46000.0 46000.0 32 -10.7 47500.0 47000.0 43000.0 33 -13.0 48000.0 47000.0 47000.0 34 -15.3 44000.0 46500.0 48000.0 35 -17.6 44000.0 48000.0 45000.0 36 -19.9 46500.0 44500.0 42500.0 37 -22.2 41000.0 44500.0 38000.0 38 -24.5 43000.0 45500.0 41500.0 39 -26.8 42000.0 42500.0 44000.0 40 -29.1 38500.0 44000.0 44500.0 41 -31.4 36000.0 41500.0 39000.0 42 -33.7 37000.0 37500.0 39500.0 43 -36.0 37000.0 39000.0 41500.0 44 -38.3 38500.0 47000.0 43000.0 45 -40.6 34500.0 35000.0 44500.0 46 -42.9 35500.0 38000.0 45500.0 47 -45.2 32000.0 40000.0 39000.0 48 -47.5 34500.0 34000.0 37000.0 49 -49.8 40500.0 33500.0 40000.0 50 -52.1 34500.0 40500.0 41500.0 51 -54.4 32000.0 40000.0 32500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 122.012u 1.371s 2:04.67 98.9% 0+0k 0+0io 0pf+0w