DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation/series0009_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 33152.1, 49728.12, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -49.1 34500.0 42500.0 47500.0 1 -46.8 47500.0 49000.0 48500.0 2 -44.5 38000.0 46500.0 34000.0 3 -42.2 49000.0 33000.0 36000.0 4 -39.9 33000.0 33000.0 33000.0 5 -37.6 39000.0 37000.0 37000.0 6 -35.3 36000.0 39000.0 33000.0 7 -33.0 33000.0 37000.0 33500.0 8 -30.7 33000.0 33000.0 35500.0 9 -28.4 41000.0 41000.0 35500.0 10 -26.1 39500.0 33500.0 33500.0 11 -23.8 33500.0 36000.0 37500.0 12 -21.5 35000.0 33000.0 33000.0 13 -19.2 34000.0 37000.0 38000.0 14 -16.9 36500.0 33500.0 44000.0 15 -14.6 37000.0 35000.0 795828275402873241600.0 16 -12.3 38000.0 37000.0 36000.0 17 -10.0 37500.0 33500.0 36000.0 18 -10.0 33000.0 33500.0 34000.0 19 -7.7 33500.0 35000.0 33000.0 20 -5.4 34000.0 34000.0 34000.0 21 -3.1 35000.0 33500.0 49000.0 22 -0.8 33000.0 34500.0 33000.0 23 1.5 34500.0 34000.0 34500.0 24 3.8 33500.0 33000.0 34500.0 25 6.1 33000.0 33000.0 33000.0 26 8.4 33000.0 33500.0 33000.0 27 10.7 49500.0 33000.0 34500.0 28 13.0 38000.0 33000.0 33000.0 29 15.3 34000.0 33500.0 34000.0 30 17.6 49500.0 33000.0 37500.0 31 19.9 46000.0 33000.0 35500.0 32 22.2 46000.0 36000.0 43000.0 33 24.5 47000.0 33000.0 33000.0 34 26.8 47000.0 44500.0 48500.0 35 29.1 43500.0 36000.0 49500.0 36 31.4 48000.0 49500.0 43000.0 37 33.7 49500.0 46500.0 37000.0 38 36.0 48500.0 40500.0 45000.0 39 38.3 33500.0 33000.0 41000.0 40 40.6 38500.0 38500.0 48000.0 41 42.9 43000.0 39500.0 42000.0 42 45.2 37000.0 33000.0 37000.0 43 47.5 38500.0 45000.0 44500.0 44 49.8 47000.0 36000.0 33000.0 45 52.1 40000.0 33000.0 33500.0 46 54.4 35500.0 35500.0 34500.0 47 56.7 38500.0 43500.0 44500.0 48 59.0 38500.0 44000.0 36000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 121.916u 1.345s 2:10.46 94.4% 0+0k 0+0io 0pf+0w