DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation/series0009_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0009/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 33152.1, 49728.12, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 49.1 34500.0 42500.0 47500.0 1 46.8 47500.0 49000.0 48500.0 2 44.5 38000.0 46500.0 34000.0 3 42.2 49000.0 33000.0 36000.0 4 39.9 33000.0 33000.0 33000.0 5 37.6 39000.0 37000.0 37000.0 6 35.3 36000.0 39000.0 33000.0 7 33.0 33000.0 37000.0 33500.0 8 30.7 33000.0 33000.0 35500.0 9 28.4 41000.0 41000.0 35500.0 10 26.1 39500.0 33500.0 33500.0 11 23.8 33500.0 36000.0 37500.0 12 21.5 35000.0 33000.0 33000.0 13 19.2 34000.0 37000.0 38000.0 14 16.9 36500.0 33500.0 44000.0 15 14.6 37000.0 35000.0 795828275402873241600.0 16 12.3 38000.0 37000.0 36000.0 17 10.0 37500.0 33500.0 36000.0 18 10.0 33000.0 33500.0 34000.0 19 7.7 33500.0 35000.0 33000.0 20 5.4 34000.0 34000.0 34000.0 21 3.1 35000.0 33500.0 49000.0 22 0.8 33000.0 34500.0 33000.0 23 -1.5 34500.0 34000.0 34500.0 24 -3.8 33500.0 33000.0 34500.0 25 -6.1 33000.0 33000.0 33000.0 26 -8.4 33000.0 33500.0 33000.0 27 -10.7 49500.0 33000.0 34500.0 28 -13.0 38000.0 33000.0 33000.0 29 -15.3 34000.0 33500.0 34000.0 30 -17.6 49500.0 33000.0 37500.0 31 -19.9 46000.0 33000.0 35500.0 32 -22.2 46000.0 36000.0 43000.0 33 -24.5 47000.0 33000.0 33000.0 34 -26.8 47000.0 44500.0 48500.0 35 -29.1 43500.0 36000.0 49500.0 36 -31.4 48000.0 49500.0 43000.0 37 -33.7 49500.0 46500.0 37000.0 38 -36.0 48500.0 40500.0 45000.0 39 -38.3 33500.0 33000.0 41000.0 40 -40.6 38500.0 38500.0 48000.0 41 -42.9 43000.0 39500.0 42000.0 42 -45.2 37000.0 33000.0 37000.0 43 -47.5 38500.0 45000.0 44500.0 44 -49.8 47000.0 36000.0 33000.0 45 -52.1 40000.0 33000.0 33500.0 46 -54.4 35500.0 35500.0 34500.0 47 -56.7 38500.0 43500.0 44500.0 48 -59.0 38500.0 44000.0 36000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 122.457u 1.201s 2:04.22 99.5% 0+0k 0+0io 0pf+0w