DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015/defocus_estimation/series0015_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32168.3, 48252.48, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 44.5 41500.0 32000.0 48000.0 1 42.2 34000.0 35000.0 34500.0 2 39.9 46500.0 38500.0 32000.0 3 37.6 40500.0 37500.0 33000.0 4 35.3 39000.0 38500.0 33500.0 5 33.0 35500.0 37500.0 36500.0 6 30.7 43000.0 36000.0 36500.0 7 28.4 32000.0 38500.0 37500.0 8 26.1 35500.0 37500.0 44000.0 9 23.8 33500.0 36500.0 41500.0 10 21.5 32000.0 36000.0 35500.0 11 19.2 32000.0 41500.0 37000.0 12 16.9 44000.0 38500.0 38500.0 13 14.6 32000.0 36000.0 34500.0 14 12.3 38500.0 40000.0 33000.0 15 10.0 32000.0 37000.0 42000.0 16 10.0 41000.0 32000.0 33500.0 17 7.7 38500.0 32000.0 32000.0 18 5.4 32500.0 38000.0 37000.0 19 3.1 35000.0 32000.0 37500.0 20 0.8 33500.0 32000.0 35000.0 21 -1.5 38500.0 41500.0 33500.0 22 -3.8 42000.0 34500.0 32000.0 23 -6.1 39000.0 39000.0 39000.0 24 -8.4 35000.0 35500.0 33000.0 25 -10.7 38500.0 36500.0 36000.0 26 -13.0 34500.0 35500.0 32000.0 27 -15.3 38500.0 36000.0 37500.0 28 -17.6 33000.0 36000.0 32000.0 29 -19.9 35000.0 32000.0 43500.0 30 -22.2 33500.0 35000.0 35000.0 31 -24.5 38500.0 35000.0 38500.0 32 -26.8 38000.0 36000.0 33000.0 33 -29.1 34000.0 38500.0 44000.0 34 -31.4 32000.0 35000.0 39000.0 35 -33.7 35500.0 35500.0 36000.0 36 -36.0 36000.0 35000.0 36000.0 37 -38.3 37000.0 35000.0 35000.0 38 -40.6 33000.0 32000.0 41000.0 39 -42.9 33500.0 40000.0 44000.0 40 -45.2 37000.0 36000.0 33000.0 41 -47.5 48000.0 43500.0 46000.0 42 -49.8 37000.0 32000.0 32500.0 43 -52.1 36000.0 34000.0 39500.0 44 -54.4 39000.0 32500.0 46500.0 45 -56.7 33500.0 37000.0 37000.0 46 -59.0 33500.0 33000.0 35500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 114.015u 1.305s 2:01.37 95.0% 0+0k 0+0io 0pf+0w