DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015_thick1600/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015_thick1600/defocus_estimation/series0015_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0015_thick1600/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 28800.0, 43200.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 39.9 28500.0 38500.0 37000.0 1 37.6 30000.0 30000.0 32500.0 2 35.3 29500.0 28500.0 33500.0 3 33.0 32500.0 30500.0 30000.0 4 30.7 28500.0 29000.0 37000.0 5 28.4 28500.0 30500.0 42500.0 6 26.1 28500.0 29000.0 28500.0 7 23.8 42500.0 37000.0 30000.0 8 21.5 32000.0 37500.0 29500.0 9 19.2 31500.0 29000.0 30000.0 10 16.9 31000.0 28500.0 35500.0 11 14.6 31000.0 30000.0 36000.0 12 12.3 28500.0 39500.0 35500.0 13 10.0 31500.0 37000.0 28500.0 14 10.0 29000.0 31000.0 34000.0 15 7.7 28500.0 28500.0 29000.0 16 5.4 28500.0 29000.0 30000.0 17 3.1 28500.0 28500.0 32500.0 18 0.8 34500.0 29500.0 28500.0 19 -1.5 28500.0 28500.0 28500.0 20 -3.8 34500.0 29000.0 32000.0 21 -6.1 29000.0 28500.0 38500.0 22 -8.4 31000.0 29000.0 28500.0 23 -10.7 29000.0 36500.0 36000.0 24 -13.0 34500.0 28500.0 28500.0 25 -15.3 28500.0 30000.0 37000.0 26 -17.6 28500.0 29000.0 30500.0 27 -19.9 35500.0 32000.0 29000.0 28 -22.2 33500.0 28500.0 28500.0 29 -24.5 28500.0 34500.0 34500.0 30 -26.8 28500.0 29500.0 33500.0 31 -29.1 38500.0 35500.0 29500.0 32 -31.4 29500.0 29500.0 40000.0 33 -33.7 35500.0 28500.0 36500.0 34 -36.0 36000.0 28500.0 30500.0 35 -38.3 37000.0 35000.0 28500.0 36 -40.6 33000.0 28500.0 30500.0 37 -42.9 33000.0 29000.0 29500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 78.291u 0.874s 1:19.24 99.8% 0+0k 0+0io 0pf+0w