DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation/series0016_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32527.0, 48790.49, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -51.4 36500.0 41500.0 43000.0 1 -49.1 42000.0 33000.0 38500.0 2 -46.8 33500.0 33000.0 39000.0 3 -44.5 37500.0 35000.0 43000.0 4 -42.2 38500.0 41000.0 33000.0 5 -39.9 38000.0 32500.0 33500.0 6 -37.6 43000.0 46500.0 46500.0 7 -35.3 43500.0 33000.0 40500.0 8 -33.0 32500.0 34500.0 33500.0 9 -30.7 40000.0 32500.0 32500.0 10 -28.4 40000.0 34000.0 38500.0 11 -26.1 34500.0 36500.0 34500.0 12 -23.8 32500.0 42500.0 38500.0 13 -21.5 34000.0 37000.0 33000.0 14 -19.2 38000.0 38500.0 32500.0 15 -16.9 48500.0 33000.0 41000.0 16 -14.6 38500.0 33500.0 32500.0 17 -12.3 36500.0 36000.0 34000.0 18 -10.0 47500.0 37000.0 32500.0 19 -10.0 34500.0 41500.0 34000.0 20 -7.7 38000.0 45500.0 35000.0 21 -5.4 38500.0 37500.0 42000.0 22 -3.1 38500.0 42000.0 40500.0 23 -0.8 33000.0 37500.0 42000.0 24 1.5 36000.0 40500.0 36000.0 25 3.8 40000.0 39000.0 44000.0 26 6.1 39000.0 37000.0 35500.0 27 8.4 35500.0 38500.0 39000.0 28 10.7 38500.0 39500.0 44500.0 29 13.0 45000.0 36500.0 37500.0 30 15.3 36000.0 37000.0 48000.0 31 17.6 33500.0 39000.0 36000.0 32 19.9 32500.0 42500.0 38500.0 33 22.2 36500.0 38000.0 46000.0 34 24.5 39000.0 36500.0 43000.0 35 26.8 33000.0 40500.0 45000.0 36 29.1 42500.0 45500.0 37500.0 37 31.4 41000.0 47500.0 44000.0 38 33.7 45500.0 32500.0 33500.0 39 36.0 48500.0 40000.0 36000.0 40 38.3 38500.0 33000.0 41500.0 41 40.6 44500.0 33500.0 42500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 95.202u 0.946s 1:36.30 99.8% 0+0k 0+0io 0pf+0w