DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation/series0016_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0016/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32527.0, 48790.49, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 51.4 36500.0 41500.0 43000.0 1 49.1 42000.0 33000.0 38500.0 2 46.8 33500.0 33000.0 39000.0 3 44.5 37500.0 35000.0 43000.0 4 42.2 38500.0 41000.0 33000.0 5 39.9 38000.0 32500.0 33500.0 6 37.6 43000.0 46500.0 46500.0 7 35.3 43500.0 33000.0 40500.0 8 33.0 32500.0 34500.0 33500.0 9 30.7 40000.0 32500.0 32500.0 10 28.4 40000.0 34000.0 38500.0 11 26.1 34500.0 36500.0 34500.0 12 23.8 32500.0 42500.0 38500.0 13 21.5 34000.0 37000.0 33000.0 14 19.2 38000.0 38500.0 32500.0 15 16.9 48500.0 33000.0 41000.0 16 14.6 38500.0 33500.0 32500.0 17 12.3 36500.0 36000.0 34000.0 18 10.0 47500.0 37000.0 32500.0 19 10.0 34500.0 41500.0 34000.0 20 7.7 38000.0 45500.0 35000.0 21 5.4 38500.0 37500.0 42000.0 22 3.1 38500.0 42000.0 40500.0 23 0.8 33000.0 37500.0 42000.0 24 -1.5 36000.0 40500.0 36000.0 25 -3.8 40000.0 39000.0 44000.0 26 -6.1 39000.0 37000.0 35500.0 27 -8.4 35500.0 38500.0 39000.0 28 -10.7 38500.0 39500.0 44500.0 29 -13.0 45000.0 36500.0 37500.0 30 -15.3 36000.0 37000.0 48000.0 31 -17.6 33500.0 39000.0 36000.0 32 -19.9 32500.0 42500.0 38500.0 33 -22.2 36500.0 38000.0 46000.0 34 -24.5 39000.0 36500.0 43000.0 35 -26.8 33000.0 40500.0 45000.0 36 -29.1 42500.0 45500.0 37500.0 37 -31.4 41000.0 47500.0 44000.0 38 -33.7 45500.0 32500.0 33500.0 39 -36.0 48500.0 40000.0 36000.0 40 -38.3 38500.0 33000.0 41500.0 41 -40.6 44500.0 33500.0 42500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 91.262u 0.904s 1:32.31 99.8% 0+0k 0+0io 0pf+0w