DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0017/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0017/defocus_estimation/series0017_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0017/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32962.5, 49443.75, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 53.7 33500.0 32500.0 43000.0 1 51.4 37500.0 32500.0 33000.0 2 49.1 38500.0 32500.0 48000.0 3 46.8 37000.0 32500.0 38500.0 4 44.5 36500.0 33000.0 32500.0 5 42.2 38500.0 35500.0 35500.0 6 39.9 38500.0 33000.0 34500.0 7 37.6 37000.0 33000.0 33000.0 8 35.3 37000.0 35000.0 32500.0 9 33.0 38000.0 35500.0 41000.0 10 30.7 38500.0 36500.0 38000.0 11 28.4 39500.0 40000.0 43000.0 12 26.1 38500.0 37500.0 40500.0 13 23.8 38500.0 37000.0 34500.0 14 21.5 41500.0 39500.0 37000.0 15 19.2 40500.0 39500.0 36500.0 16 16.9 39000.0 39500.0 38500.0 17 14.6 41500.0 41000.0 140563005308928.0 18 12.3 42000.0 41000.0 45500.0 19 10.0 41500.0 41500.0 40500.0 20 10.0 43500.0 43500.0 40500.0 21 7.7 41500.0 42500.0 43500.0 22 5.4 41500.0 44000.0 43000.0 23 3.1 40500.0 40500.0 42000.0 24 0.8 40500.0 42500.0 41500.0 25 -1.5 43000.0 41000.0 41000.0 26 -3.8 40000.0 40500.0 42500.0 27 -6.1 41000.0 41000.0 41000.0 28 -8.4 41000.0 41000.0 41500.0 29 -10.7 40500.0 42000.0 42000.0 30 -13.0 39000.0 42500.0 44500.0 31 -15.3 39000.0 41000.0 40000.0 32 -17.6 39500.0 41500.0 43000.0 33 -19.9 38000.0 39000.0 41000.0 34 -22.2 38500.0 38000.0 42500.0 35 -24.5 37000.0 39000.0 40000.0 36 -26.8 36500.0 37000.0 39500.0 37 -29.1 34500.0 38500.0 39000.0 38 -31.4 33000.0 38000.0 40000.0 39 -33.7 35000.0 36000.0 39500.0 40 -36.0 32500.0 36500.0 38500.0 41 -38.3 35500.0 35000.0 39000.0 42 -40.6 32500.0 34000.0 36000.0 43 -42.9 35500.0 33500.0 37000.0 44 -45.2 38500.0 35500.0 41500.0 45 -47.5 34000.0 49000.0 49000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 112.891u 1.197s 1:59.97 95.0% 0+0k 0+0io 0pf+0w