DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0018/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0018/defocus_estimation/series0018_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0018/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32282.0, 48422.93, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 56.0 37000.0 33000.0 34000.0 1 53.7 40500.0 34000.0 32000.0 2 51.4 40500.0 34000.0 32000.0 3 49.1 39000.0 35500.0 33500.0 4 46.8 39500.0 34500.0 32500.0 5 44.5 37500.0 35000.0 34000.0 6 42.2 39500.0 35500.0 32000.0 7 39.9 40000.0 35000.0 34500.0 8 37.6 39000.0 36500.0 35000.0 9 35.3 39000.0 38500.0 34500.0 10 33.0 40000.0 36000.0 33500.0 11 30.7 39500.0 37500.0 35000.0 12 28.4 41500.0 37000.0 34500.0 13 26.1 40500.0 40500.0 36000.0 14 23.8 43500.0 42000.0 38500.0 15 21.5 40500.0 43000.0 42500.0 16 19.2 42500.0 39500.0 40500.0 17 16.9 46000.0 40500.0 42500.0 18 14.6 48000.0 41000.0 0.0 19 12.3 42500.0 43000.0 40500.0 20 10.0 45000.0 43000.0 41500.0 21 10.0 42500.0 42000.0 40500.0 22 7.7 40500.0 40500.0 42500.0 23 5.4 43000.0 42000.0 42500.0 24 3.1 42000.0 41000.0 41500.0 25 0.8 41500.0 41000.0 44000.0 26 -1.5 43500.0 42500.0 45000.0 27 -3.8 40500.0 41500.0 42500.0 28 -6.1 41000.0 42000.0 41500.0 29 -8.4 41000.0 41500.0 42500.0 30 -10.7 40500.0 41500.0 42000.0 31 -13.0 40000.0 41000.0 42500.0 32 -15.3 40000.0 40500.0 40000.0 33 -17.6 38000.0 44500.0 44500.0 34 -19.9 37500.0 39500.0 41500.0 35 -22.2 36500.0 40000.0 39500.0 36 -24.5 37000.0 38000.0 38500.0 37 -26.8 35000.0 38500.0 39500.0 38 -29.1 35000.0 37000.0 43500.0 39 -31.4 34500.0 36000.0 39500.0 40 -33.7 32500.0 36000.0 40000.0 41 -36.0 32500.0 41000.0 38500.0 42 -38.3 32000.0 34000.0 38500.0 43 -40.6 37000.0 33000.0 37000.0 44 -42.9 32000.0 33500.0 40500.0 45 -45.2 40000.0 33000.0 42500.0 46 -47.5 48000.0 34500.0 32500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 98.188u 1.255s 1:39.66 99.7% 0+0k 0+0io 0pf+0w