DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation/series0019_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32168.3, 48252.38, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -56.0 33000.0 37500.0 47000.0 1 -53.7 32000.0 32000.0 47500.0 2 -51.4 33000.0 32000.0 36000.0 3 -49.1 36000.0 40000.0 47000.0 4 -46.8 39500.0 35500.0 33000.0 5 -44.5 40500.0 32000.0 36000.0 6 -42.2 48000.0 32000.0 32000.0 7 -39.9 35000.0 32000.0 41000.0 8 -37.6 41500.0 34000.0 32000.0 9 -35.3 42500.0 35000.0 32000.0 10 -33.0 39000.0 32000.0 46000.0 11 -30.7 42500.0 33500.0 32000.0 12 -28.4 39500.0 32000.0 36000.0 13 -26.1 32000.0 32500.0 42500.0 14 -23.8 44500.0 33000.0 36500.0 15 -21.5 38500.0 33000.0 32500.0 16 -19.2 38000.0 41000.0 38500.0 17 -16.9 46500.0 35000.0 35000.0 18 -14.6 36000.0 38500.0 46000.0 19 -12.3 37000.0 36000.0 37500.0 20 -10.0 46000.0 42500.0 40500.0 21 -10.0 38000.0 38000.0 39000.0 22 -7.7 32500.0 43500.0 45500.0 23 -5.4 36500.0 40000.0 37000.0 24 -3.1 46500.0 41500.0 41500.0 25 -0.8 38500.0 41000.0 37500.0 26 1.5 38500.0 40500.0 37000.0 27 3.8 37500.0 41500.0 37500.0 28 6.1 38000.0 37000.0 40000.0 29 8.4 40000.0 39000.0 37500.0 30 10.7 45500.0 37500.0 40000.0 31 13.0 47500.0 39000.0 38500.0 32 15.3 35000.0 44000.0 41500.0 33 17.6 37500.0 38500.0 39500.0 34 19.9 35500.0 35000.0 37500.0 35 22.2 43000.0 35500.0 40500.0 36 24.5 34000.0 34500.0 38500.0 37 26.8 32500.0 34000.0 37000.0 38 29.1 37500.0 36000.0 37000.0 39 31.4 33500.0 36500.0 36000.0 40 33.7 38000.0 38500.0 35500.0 41 36.0 32500.0 33000.0 36000.0 42 38.3 32500.0 33000.0 35000.0 43 40.6 37000.0 44000.0 43000.0 44 42.9 33500.0 34500.0 44000.0 45 45.2 44500.0 39500.0 37500.0 46 47.5 39500.0 39500.0 40500.0 47 49.8 40500.0 41000.0 34000.0 48 52.1 45000.0 44000.0 35000.0 49 54.4 33500.0 38500.0 39500.0 50 56.7 34500.0 39500.0 46000.0 51 59.0 32000.0 37000.0 48000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 119.252u 1.150s 2:00.62 99.8% 0+0k 0+0io 0pf+0w