DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation/series0019_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0019/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32168.3, 48252.38, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 56.0 33000.0 37500.0 47000.0 1 53.7 32000.0 32000.0 47500.0 2 51.4 33000.0 32000.0 36000.0 3 49.1 36000.0 40000.0 47000.0 4 46.8 39500.0 35500.0 33000.0 5 44.5 40500.0 32000.0 36000.0 6 42.2 48000.0 32000.0 32000.0 7 39.9 35000.0 32000.0 41000.0 8 37.6 41500.0 34000.0 32000.0 9 35.3 42500.0 35000.0 32000.0 10 33.0 39000.0 32000.0 46000.0 11 30.7 42500.0 33500.0 32000.0 12 28.4 39500.0 32000.0 36000.0 13 26.1 32000.0 32500.0 42500.0 14 23.8 44500.0 33000.0 36500.0 15 21.5 38500.0 33000.0 32500.0 16 19.2 38000.0 41000.0 38500.0 17 16.9 46500.0 35000.0 35000.0 18 14.6 36000.0 38500.0 46000.0 19 12.3 37000.0 36000.0 37500.0 20 10.0 46000.0 42500.0 40500.0 21 10.0 38000.0 38000.0 39000.0 22 7.7 32500.0 43500.0 45500.0 23 5.4 36500.0 40000.0 37000.0 24 3.1 46500.0 41500.0 41500.0 25 0.8 38500.0 41000.0 37500.0 26 -1.5 38500.0 40500.0 37000.0 27 -3.8 37500.0 41500.0 37500.0 28 -6.1 38000.0 37000.0 40000.0 29 -8.4 40000.0 39000.0 37500.0 30 -10.7 45500.0 37500.0 40000.0 31 -13.0 47500.0 39000.0 38500.0 32 -15.3 35000.0 44000.0 41500.0 33 -17.6 37500.0 38500.0 39500.0 34 -19.9 35500.0 35000.0 37500.0 35 -22.2 43000.0 35500.0 40500.0 36 -24.5 34000.0 34500.0 38500.0 37 -26.8 32500.0 34000.0 37000.0 38 -29.1 37500.0 36000.0 37000.0 39 -31.4 33500.0 36500.0 36000.0 40 -33.7 38000.0 38500.0 35500.0 41 -36.0 32500.0 33000.0 36000.0 42 -38.3 32500.0 33000.0 35000.0 43 -40.6 37000.0 44000.0 43000.0 44 -42.9 33500.0 34500.0 44000.0 45 -45.2 44500.0 39500.0 37500.0 46 -47.5 39500.0 39500.0 40500.0 47 -49.8 40500.0 41000.0 34000.0 48 -52.1 45000.0 44000.0 35000.0 49 -54.4 33500.0 38500.0 39500.0 50 -56.7 34500.0 39500.0 46000.0 51 -59.0 32000.0 37000.0 48000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 119.371u 1.501s 2:06.97 95.1% 0+0k 0+0io 0pf+0w