DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation/series0020_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32964.4, 49446.63, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -42.2 34500.0 38000.0 32500.0 1 -39.9 44500.0 36000.0 33000.0 2 -37.6 39500.0 37500.0 36000.0 3 -35.3 40500.0 38500.0 35000.0 4 -33.0 42000.0 38500.0 36500.0 5 -30.7 40500.0 37500.0 38500.0 6 -28.4 41500.0 39500.0 34500.0 7 -26.1 42500.0 39000.0 35000.0 8 -23.8 45000.0 41500.0 38000.0 9 -21.5 42500.0 41500.0 37500.0 10 -19.2 43500.0 42500.0 40500.0 11 -16.9 43000.0 41500.0 41000.0 12 -14.6 44000.0 43000.0 0.0 13 -12.3 45500.0 44500.0 43000.0 14 -10.0 45500.0 45500.0 43500.0 15 -10.0 44000.0 44500.0 44000.0 16 -7.7 45500.0 44000.0 44500.0 17 -5.4 43000.0 44500.0 44000.0 18 -3.1 44000.0 44500.0 43500.0 19 -0.8 42000.0 42500.0 43000.0 20 1.5 44000.0 43000.0 44500.0 21 3.8 42500.0 42500.0 43000.0 22 6.1 43000.0 42500.0 43500.0 23 8.4 41500.0 43500.0 43000.0 24 10.7 41000.0 42500.0 45000.0 25 13.0 41500.0 44000.0 43500.0 26 15.3 41000.0 42000.0 43000.0 27 17.6 42500.0 42000.0 44500.0 28 19.9 38500.0 40000.0 39000.0 29 22.2 37500.0 40000.0 40500.0 30 24.5 37500.0 39000.0 40500.0 31 26.8 38000.0 39500.0 41000.0 32 29.1 36000.0 37500.0 42500.0 33 31.4 34500.0 38500.0 40500.0 34 33.7 35500.0 37000.0 40000.0 35 36.0 33500.0 35000.0 40000.0 36 38.3 32500.0 34500.0 40000.0 37 40.6 33000.0 34000.0 38500.0 38 42.9 37000.0 35500.0 39500.0 39 45.2 34000.0 35000.0 37000.0 40 47.5 49000.0 32500.0 35000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 93.234u 0.885s 1:34.24 99.8% 0+0k 0+0io 0pf+0w