DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation/series0020_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0020/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32964.4, 49446.63, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 42.2 34500.0 38000.0 32500.0 1 39.9 44500.0 36000.0 33000.0 2 37.6 39500.0 37500.0 36000.0 3 35.3 40500.0 38500.0 35000.0 4 33.0 42000.0 38500.0 36500.0 5 30.7 40500.0 37500.0 38500.0 6 28.4 41500.0 39500.0 34500.0 7 26.1 42500.0 39000.0 35000.0 8 23.8 45000.0 41500.0 38000.0 9 21.5 42500.0 41500.0 37500.0 10 19.2 43500.0 42500.0 40500.0 11 16.9 43000.0 41500.0 41000.0 12 14.6 44000.0 43000.0 0.0 13 12.3 45500.0 44500.0 43000.0 14 10.0 45500.0 45500.0 43500.0 15 10.0 44000.0 44500.0 44000.0 16 7.7 45500.0 44000.0 44500.0 17 5.4 43000.0 44500.0 44000.0 18 3.1 44000.0 44500.0 43500.0 19 0.8 42000.0 42500.0 43000.0 20 -1.5 44000.0 43000.0 44500.0 21 -3.8 42500.0 42500.0 43000.0 22 -6.1 43000.0 42500.0 43500.0 23 -8.4 41500.0 43500.0 43000.0 24 -10.7 41000.0 42500.0 45000.0 25 -13.0 41500.0 44000.0 43500.0 26 -15.3 41000.0 42000.0 43000.0 27 -17.6 42500.0 42000.0 44500.0 28 -19.9 38500.0 40000.0 39000.0 29 -22.2 37500.0 40000.0 40500.0 30 -24.5 37500.0 39000.0 40500.0 31 -26.8 38000.0 39500.0 41000.0 32 -29.1 36000.0 37500.0 42500.0 33 -31.4 34500.0 38500.0 40500.0 34 -33.7 35500.0 37000.0 40000.0 35 -36.0 33500.0 35000.0 40000.0 36 -38.3 32500.0 34500.0 40000.0 37 -40.6 33000.0 34000.0 38500.0 38 -42.9 37000.0 35500.0 39500.0 39 -45.2 34000.0 35000.0 37000.0 40 -47.5 49000.0 32500.0 35000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 93.217u 1.113s 1:39.07 95.2% 0+0k 0+0io 0pf+0w