DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation/series0021_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32101.9, 48152.81, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -58.3 32500.0 32500.0 44000.0 1 -56.0 33500.0 38000.0 43500.0 2 -53.7 33000.0 32000.0 33500.0 3 -51.4 41000.0 37000.0 34000.0 4 -49.1 36000.0 32000.0 46500.0 5 -46.8 35000.0 36000.0 46500.0 6 -44.5 35000.0 36000.0 47500.0 7 -42.2 40000.0 36000.0 47500.0 8 -39.9 37500.0 40500.0 48000.0 9 -37.6 40000.0 35500.0 37500.0 10 -35.3 37500.0 34000.0 48000.0 11 -33.0 35500.0 32000.0 45500.0 12 -30.7 32000.0 33000.0 33000.0 13 -28.4 32000.0 32500.0 44500.0 14 -26.1 38500.0 35000.0 36000.0 15 -23.8 32000.0 34500.0 48000.0 16 -21.5 40500.0 37500.0 32000.0 17 -19.2 43000.0 37500.0 34500.0 18 -16.9 33500.0 34500.0 38000.0 19 -14.6 36000.0 39000.0 35500.0 20 -12.3 36500.0 34500.0 32000.0 21 -10.0 33000.0 35000.0 32500.0 22 -10.0 35000.0 34500.0 34500.0 23 -7.7 35500.0 34500.0 34500.0 24 -5.4 36000.0 35500.0 34000.0 25 -3.1 34500.0 36000.0 37500.0 26 -0.8 34500.0 35000.0 33500.0 27 1.5 36000.0 32000.0 37500.0 28 3.8 34500.0 32000.0 32000.0 29 6.1 34500.0 32000.0 40500.0 30 8.4 32000.0 34500.0 39500.0 31 10.7 33500.0 35500.0 32000.0 32 13.0 36000.0 36000.0 32000.0 33 15.3 34000.0 34500.0 36000.0 34 17.6 39500.0 35000.0 36500.0 35 19.9 36500.0 41000.0 36000.0 36 22.2 35000.0 34500.0 38500.0 37 24.5 41500.0 35500.0 39500.0 38 26.8 37000.0 32000.0 32000.0 39 29.1 38000.0 41500.0 32000.0 40 31.4 34500.0 38500.0 40000.0 41 33.7 32000.0 37500.0 39000.0 42 36.0 38500.0 32000.0 38000.0 43 38.3 32500.0 35500.0 44500.0 44 40.6 32000.0 38500.0 45000.0 45 42.9 37500.0 42500.0 44500.0 46 45.2 32000.0 36000.0 33000.0 47 47.5 42000.0 34000.0 33000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 116.220u 1.037s 1:58.78 98.7% 0+0k 0+0io 0pf+0w