DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation/series0021_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32101.9, 48152.81, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 58.3 32500.0 32500.0 44000.0 1 56.0 33500.0 38000.0 43500.0 2 53.7 33000.0 32000.0 33500.0 3 51.4 41000.0 37000.0 34000.0 4 49.1 36000.0 32000.0 46500.0 5 46.8 35000.0 36000.0 46500.0 6 44.5 35000.0 36000.0 47500.0 7 42.2 40000.0 36000.0 47500.0 8 39.9 37500.0 40500.0 48000.0 9 37.6 40000.0 35500.0 37500.0 10 35.3 37500.0 34000.0 48000.0 11 33.0 35500.0 32000.0 45500.0 12 30.7 32000.0 33000.0 33000.0 13 28.4 32000.0 32500.0 44500.0 14 26.1 38500.0 35000.0 36000.0 15 23.8 32000.0 34500.0 48000.0 16 21.5 40500.0 37500.0 32000.0 17 19.2 43000.0 37500.0 34500.0 18 16.9 33500.0 34500.0 38000.0 19 14.6 36000.0 39000.0 35500.0 20 12.3 36500.0 34500.0 32000.0 21 10.0 33000.0 35000.0 32500.0 22 10.0 35000.0 34500.0 34500.0 23 7.7 35500.0 34500.0 34500.0 24 5.4 36000.0 35500.0 34000.0 25 3.1 34500.0 36000.0 37500.0 26 0.8 34500.0 35000.0 33500.0 27 -1.5 36000.0 32000.0 37500.0 28 -3.8 34500.0 32000.0 32000.0 29 -6.1 34500.0 32000.0 40500.0 30 -8.4 32000.0 34500.0 39500.0 31 -10.7 33500.0 35500.0 32000.0 32 -13.0 36000.0 36000.0 32000.0 33 -15.3 34000.0 34500.0 36000.0 34 -17.6 39500.0 35000.0 36500.0 35 -19.9 36500.0 41000.0 36000.0 36 -22.2 35000.0 34500.0 38500.0 37 -24.5 41500.0 35500.0 39500.0 38 -26.8 37000.0 32000.0 32000.0 39 -29.1 38000.0 41500.0 32000.0 40 -31.4 34500.0 38500.0 40000.0 41 -33.7 32000.0 37500.0 39000.0 42 -36.0 38500.0 32000.0 38000.0 43 -38.3 32500.0 35500.0 44500.0 44 -40.6 32000.0 38500.0 45000.0 45 -42.9 37500.0 42500.0 44500.0 46 -45.2 32000.0 36000.0 33000.0 47 -47.5 42000.0 34000.0 33000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 116.666u 1.343s 2:02.64 96.2% 0+0k 0+0io 0pf+0w