protomo2aligner.py --my_tlt=False --r5_kernel_x=5 --r5_iters=0 --r4_mask_apod_y=10 --r4_mask_apod_x=10 --corr_mode=mcf --rundir=/gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0021_thick1800 --r1_peak_search_radius_y=300.0 --r1_peak_search_radius_x=300.0 --serialem_stack= --r3_highpass_diameter_x=7690.0 --r4_lowpass_diameter_x=11.2 --filename_prefix= --r4_cmdiameter_x=100.0 --r4_cmdiameter_y=100.0 --r1_region_x=2749 --r1_region_y=3017 --cmdiameter_y=100.0 --r2_corr_mode=mcf --r5_highpass_diameter_x=7690.0 --r4_lowpass_apod_y=28.0 --r4_lowpass_apod_x=28.0 --r3_kernel_y=5 --r3_kernel_x=5 --r3_lowpass_apod_x=56.0 --r3_lowpass_apod_y=56.0 --manual_alignment_finished=False --r5_body=0 --runname=tiltseries0021_thick1800 --r2_region_y=3017 --r2_region_x=2749 --r2_sampling=4 --video_type=html5vid --rotation=true --r2_highpass_apod_y=3845.0 --defocus_ang_positive=90 --r5_mask_width_x=2724 --frame_aligned=True --image_file_type=mrc --r3_highpass_diameter_y=7690.0 --r3_highpass_apod_x=3845.0 --r5_cmdiameter_y=100.0 --r5_cmdiameter_x=100.0 --r2_mask_width_x=662 --r2_mask_width_y=729 --r5_region_x=2749 --r5_region_y=3017 --highpass_diameter_y=0.001 --highpass_diameter_x=0.001 --create_reconstruction=true --r5_mask_apod_x=10 --r5_mask_apod_y=10 --r4_lowpass_diameter_y=11.2 --r1_body=0 --r1_mask_apod_x=10 --defocus_difference=0.2 --correlation_size_y=160 --correlation_size_x=160 --r2_cmdiameter_x=100.0 --r2_cmdiameter_y=100.0 --gridsearch_limit=2.0 --azimuth_stability_check=True --r1_highpass_diameter_y=7690.0 --r4_kernel_x=5 --r4_kernel_y=5 --amp_contrast_defocus=0.07 --peak_search_radius_x=100.0 --peak_search_radius_y=100.0 --r5_mask_width_y=2992 --window_area=0.95 --r1_corr_mode=mcf --gradient=true --create_tilt_video=true --mask_apod_y=10 --r5_peak_search_radius_y=300.0 --exclude_images_by_angle= --r3_mask_apod_x=10 --r3_mask_apod_y=10 --norotations=false --r3_highpass_apod_y=3845.0 --r3_iters=5 --r1_highpass_diameter_x=7690.0 --r2_lowpass_diameter_y=44.8 --map_size_y=463 --map_size_z=200 --r5_lowpass_diameter_y=5.6 --r5_lowpass_diameter_x=5.6 --max_shift=999999 --cachedir=cache --clip_high=3.5 --grow=0 --r3_cmdiameter_y=100.0 --r3_cmdiameter_x=100.0 --keep_recons=false --r4_mask_width_x=2724 --r4_mask_width_y=2992 --r5_highpass_apod_y=3845.0 --logging=true --DefocusTol=200 --filter=median --sessionname=19feb06g --exclude_images=999999 --commit=False --r1_kernel_y=5 --r1_kernel_x=5 --dose_presets=False --mask_apod_x=10 --r4_corr_mode=mcf --show_window_size=true --defocus_estimate=False --r4_iters=10 --amp_contrast_ctf=0.07 --r1_highpass_apod_y=3845.0 --r1_highpass_apod_x=3845.0 --r2_highpass_apod_x=3845.0 --sampling=8 --starting_tlt_file=Manual --r5_lowpass_apod_x=14.0 --r5_lowpass_apod_y=14.0 --dose_c=2.81 --r1_cmdiameter_y=100.0 --further_alignment=False --r1_lowpass_diameter_y=89.6 --r1_lowpass_diameter_x=89.6 --r4_sampling=1 --res_max=10 --r1_mask_apod_y=10 --res_min=10000 --scale_switch=31 --dose_b=-1.665 --positive=90.0 --r3_peak_search_radius_y=300.0 --r3_peak_search_radius_x=300.0 --negative=-90.0 --parallel=True --tiltseries=21 --r5_corr_mode=pcf --change_refimg=False --r3_mask_width_y=1483 --r3_mask_width_x=1349 --map_sampling=8 --thr_low=0 --gridsearch_step=0.1 --coarse=False --r2_iters=10 --region_y=512 --r4_highpass_diameter_y=7690.0 --r4_highpass_diameter_x=7690.0 --mask_width_x=1024 --highpass_apod_x=0.002 --binning=true --r1_lowpass_apod_y=224.0 --thr_high=999999 --r5_sampling=1 --highpass_apod_y=0.002 --defocus_min=0 --r1_sampling=8 --ctf_correct=False --r2_highpass_diameter_y=7690.0 --r2_mask_apod_y=10 --r2_mask_apod_x=10 --desired_ref_tilt_angle=0.0 --r5_highpass_apod_x=3845.0 --defocus_save=0 --protomo_outdir=out --r5_highpass_diameter_y=7690.0 --defocus_max=0 --r4_region_y=3017 --r4_region_x=2749 --r5_kernel_y=5 --r3_region_x=2749 --r3_region_y=3017 --r4_body=0 --serialem_mdoc= --r2_peak_search_radius_x=300.0 --r2_peak_search_radius_y=300.0 --thickness=1800 --expid=8521 --r2_body=0 --orientation=true --do_estimation=false --iter_gradient=false --projectid=282 --map_size_x=479 --r3_body=0 --r3_lowpass_diameter_y=22.4 --r3_lowpass_diameter_x=22.4 --mask_width_y=1024 --max_correction=999999 --elevation=false --r2_kernel_x=5 --r2_kernel_y=5 --slab=true --pixelsize=2.7975 --link=False --r2_highpass_diameter_x=7690.0 --lowpass_diameter_x=0.5 --lowpass_diameter_y=0.5 --defocus=3 --border=100 --r3_corr_mode=mcf --r2_lowpass_apod_y=112.0 --exclude_images_by_angle_accuracy=0.05 --r2_lowpass_apod_x=112.0 --defocus_ang_negative=-90 --r3_sampling=2 --description= --make_searchable=True --translimit=301.0 --iters=1 --r5_peak_search_radius_x=300.0 --r1_iters=10 --r1_cmdiameter_x=100.0 --preprocessing=true --scale=false --iWidth=20 --r2_lowpass_diameter_x=44.8 --dimx=3838 --dimy=3710 --r4_peak_search_radius_x=300.0 --r4_peak_search_radius_y=300.0 --r1_lowpass_apod_x=224.0 --region_x=512 --manual_alignment=False --loglevel=2 --restart_cycle=0 --dose_a=0.245 --lowpass_apod_y=0.05 --lowpass_apod_x=0.05 --kernel_x=3 --kernel_y=3 --clip_low=3.5 --azimuth_max_deviation=2.5 --tilt_clip=true --r4_highpass_apod_x=3845.0 --r4_highpass_apod_y=3845.0 --azimuth=true --r1_mask_width_y=352 --r1_mask_width_x=318 --jobtype=protomo --from_multirefine=True node37 Start time: 2019yr02m12d-11hr52m20s Description: Using alignment .tlt file as determined by protomo2multirefine.py.Starting Protomo alignment Beginning Refinements Beginning Refinement Iteration #1, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) lowpass = 89.6 Angstroms Round #1. Parameters in Protomo units: (Note: At binned by 8, pixelsize is 22.38 Angstroms, Nyquist is 44.76 Angstroms) window.lowpass.apodization = { 0.199821428571 0.199821428571 } align.translimit = 37.625e0 map.sampling = 8 window.highpass.diameter = { 0.00582054616385 0.00582054616385 } window.highpass.apodization = { 0.0116410923277 0.0116410923277 } window.mask.apodization = { 10 10 } align.mask.width = { 318 352 } sampling = 8 align.mask.apodization = { 10 10 } map.body = 153.025907739 map.lowpass.diameter = { 0.499553571429 0.499553571429 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 343 377 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 318 352 } reference.body = 153.025907739 window.lowpass.diameter = { 0.499553571429 0.499553571429 } align.correlation.mode = mcf No parameters were switched for Iteration #1 Creating quality assessment statistics... CCMS(shift) = 0.06759 for Iteration #1 of Tilt-Series #21. CCMS(rotations) = 1.10153 for Iteration #1 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #1 of Tilt-Series #21. The scaled sum of CCMS values is #0.05582 for iteration 1 of Tilt-Series #21. Alignment quality for Iteration #1 of Tilt-Series #21 (19feb06g): Bad - 1st iteration is always bad because the geometry has not yet been estimated (confidence: 100.000000%)Creating Depiction Videos for Iteration #1 in Parallel... Creating Refinement tilt-series video for iteration #1... Generating Refinement reconstruction for iteration #1... The tilt azimuth after iteration #1 deviated from the initial tilt azimuth by 1.714000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #2 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #2, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #2 Creating quality assessment statistics... CCMS(shift) = 0.0687 for Iteration #2 of Tilt-Series #21. CCMS(rotations) = 1.27192 for Iteration #2 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #2 of Tilt-Series #21. The scaled sum of CCMS values is #0.05979 for iteration 2 of Tilt-Series #21. Alignment quality for Iteration #2 of Tilt-Series #21 (19feb06g): Bad (confidence: 100.000000%)Creating Depiction Videos for Iteration #2 in Parallel... Creating Refinement tilt-series video for iteration #2... Generating Refinement reconstruction for iteration #2... The tilt azimuth after iteration #2 deviated from the initial tilt azimuth by 2.460000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #3 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #3, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #3 Creating quality assessment statistics... CCMS(shift) = 0.03747 for Iteration #3 of Tilt-Series #21. CCMS(rotations) = 0.98895 for Iteration #3 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #3 of Tilt-Series #21. The scaled sum of CCMS values is #0.03852 for iteration 3 of Tilt-Series #21. Alignment quality for Iteration #3 of Tilt-Series #21 (19feb06g): Bad (confidence: 100.000000%)Creating Depiction Videos for Iteration #3 in Parallel... Creating Refinement tilt-series video for iteration #3... Generating Refinement reconstruction for iteration #3... The tilt azimuth after iteration #3 deviated from the initial tilt azimuth by 3.062000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #4 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #4, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #4 Creating quality assessment statistics... CCMS(shift) = 0.03615 for Iteration #4 of Tilt-Series #21. CCMS(rotations) = 0.58872 for Iteration #4 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #4 of Tilt-Series #21. The scaled sum of CCMS values is #0.02985 for iteration 4 of Tilt-Series #21. Alignment quality for Iteration #4 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #4 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #4 in Parallel... Creating Refinement tilt-series video for iteration #4... Generating Refinement reconstruction for iteration #4... The tilt azimuth after iteration #4 deviated from the initial tilt azimuth by 1.559000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #5 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #5, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #5 Creating quality assessment statistics... CCMS(shift) = 0.02671 for Iteration #5 of Tilt-Series #21. CCMS(rotations) = 0.61381 for Iteration #5 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #5 of Tilt-Series #21. The scaled sum of CCMS values is #0.02563 for iteration 5 of Tilt-Series #21. Alignment quality for Iteration #5 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #5 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #5 in Parallel... Creating Refinement tilt-series video for iteration #5... Generating Refinement reconstruction for iteration #5... The tilt azimuth after iteration #5 deviated from the initial tilt azimuth by 1.989000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #6 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #6, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #6 Creating quality assessment statistics... CCMS(shift) = 0.02663 for Iteration #6 of Tilt-Series #21. CCMS(rotations) = 0.5487 for Iteration #6 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #6 of Tilt-Series #21. The scaled sum of CCMS values is #0.02429 for iteration 6 of Tilt-Series #21. Alignment quality for Iteration #6 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #6 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #6 in Parallel... Creating Refinement tilt-series video for iteration #6... Generating Refinement reconstruction for iteration #6... The tilt azimuth after iteration #6 deviated from the initial tilt azimuth by 2.084000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #7 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #7, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #7 Creating quality assessment statistics... CCMS(shift) = 0.02477 for Iteration #7 of Tilt-Series #21. CCMS(rotations) = 0.66655 for Iteration #7 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #7 of Tilt-Series #21. The scaled sum of CCMS values is #0.02572 for iteration 7 of Tilt-Series #21. Alignment quality for Iteration #7 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #7 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #7 in Parallel... Creating Refinement tilt-series video for iteration #7... Generating Refinement reconstruction for iteration #7... The tilt azimuth after iteration #7 deviated from the initial tilt azimuth by 2.118000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #8 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #8, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #8 Creating quality assessment statistics... CCMS(shift) = 0.0212 for Iteration #8 of Tilt-Series #21. CCMS(rotations) = 0.6859 for Iteration #8 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #8 of Tilt-Series #21. The scaled sum of CCMS values is #0.02432 for iteration 8 of Tilt-Series #21. Alignment quality for Iteration #8 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #8 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #8 in Parallel... Creating Refinement tilt-series video for iteration #8... Generating Refinement reconstruction for iteration #8... The tilt azimuth after iteration #8 deviated from the initial tilt azimuth by 2.192000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #9 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #9, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #9 Creating quality assessment statistics... CCMS(shift) = 0.02469 for Iteration #9 of Tilt-Series #21. CCMS(rotations) = 0.72689 for Iteration #9 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #9 of Tilt-Series #21. The scaled sum of CCMS values is #0.02689 for iteration 9 of Tilt-Series #21. Alignment quality for Iteration #9 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #9 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #9 in Parallel... Creating Refinement tilt-series video for iteration #9... Generating Refinement reconstruction for iteration #9... The tilt azimuth after iteration #9 deviated from the initial tilt azimuth by 2.198000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #10 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #10, Round #1 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #10 Creating quality assessment statistics... CCMS(shift) = 0.02332 for Iteration #10 of Tilt-Series #21. CCMS(rotations) = 0.70137 for Iteration #10 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #10 of Tilt-Series #21. The scaled sum of CCMS values is #0.02569 for iteration 10 of Tilt-Series #21. Alignment quality for Iteration #10 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #10 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #10 in Parallel... Creating Refinement tilt-series video for iteration #10... Generating Refinement reconstruction for iteration #10... The tilt azimuth after iteration #10 deviated from the initial tilt azimuth by 2.129000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #11, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) lowpass = 44.8 angstroms Round #2. Parameters in Protomo units: (Note: At binned by 4, pixelsize is 11.19 Angstroms, Nyquist is 22.38 Angstroms) window.lowpass.apodization = { 0.199821428571 0.199821428571 } align.translimit = 75.25e0 map.sampling = 4 window.highpass.diameter = { 0.00291027308192 0.00291027308192 } window.highpass.apodization = { 0.00582054616385 0.00582054616385 } window.mask.apodization = { 10 10 } align.mask.width = { 662 729 } sampling = 4 align.mask.apodization = { 10 10 } map.body = 306.051815479 map.lowpass.diameter = { 0.499553571429 0.499553571429 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 687 754 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 662 729 } reference.body = 306.051815479 window.lowpass.diameter = { 0.499553571429 0.499553571429 } align.correlation.mode = mcf No parameters were switched for Iteration #11 Creating quality assessment statistics... CCMS(shift) = 0.01732 for Iteration #11 of Tilt-Series #21. CCMS(rotations) = 0.80626 for Iteration #11 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #11 of Tilt-Series #21. The scaled sum of CCMS values is #0.02478 for iteration 11 of Tilt-Series #21. Alignment quality for Iteration #11 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #11 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #11 in Parallel... Creating Refinement tilt-series video for iteration #11... Generating Refinement reconstruction for iteration #11... The tilt azimuth after iteration #11 deviated from the initial tilt azimuth by 2.538000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #12 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #12, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #12 Creating quality assessment statistics... CCMS(shift) = 0.02943 for Iteration #12 of Tilt-Series #21. CCMS(rotations) = 0.70136 for Iteration #12 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #12 of Tilt-Series #21. The scaled sum of CCMS values is #0.02874 for iteration 12 of Tilt-Series #21. Alignment quality for Iteration #12 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #12 of Tilt-Series #21 (19feb06g): Very StableCreating Depiction Videos for Iteration #12 in Parallel... Creating Refinement tilt-series video for iteration #12... Generating Refinement reconstruction for iteration #12... The tilt azimuth after iteration #12 deviated from the initial tilt azimuth by 1.970000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #13 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #13, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #13 Creating quality assessment statistics... CCMS(shift) = 0.02093 for Iteration #13 of Tilt-Series #21. CCMS(rotations) = 0.85932 for Iteration #13 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #13 of Tilt-Series #21. The scaled sum of CCMS values is #0.02765 for iteration 13 of Tilt-Series #21. Alignment quality for Iteration #13 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #13 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #13 in Parallel... Creating Refinement tilt-series video for iteration #13... Generating Refinement reconstruction for iteration #13... The tilt azimuth after iteration #13 deviated from the initial tilt azimuth by 2.728000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #14 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #14, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #14 Creating quality assessment statistics... CCMS(shift) = 0.02962 for Iteration #14 of Tilt-Series #21. CCMS(rotations) = 0.71891 for Iteration #14 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #14 of Tilt-Series #21. The scaled sum of CCMS values is #0.02919 for iteration 14 of Tilt-Series #21. Alignment quality for Iteration #14 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #14 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #14 in Parallel... Creating Refinement tilt-series video for iteration #14... Generating Refinement reconstruction for iteration #14... The tilt azimuth after iteration #14 deviated from the initial tilt azimuth by 1.913000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #15 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #15, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #15 Creating quality assessment statistics... CCMS(shift) = 0.02245 for Iteration #15 of Tilt-Series #21. CCMS(rotations) = 0.83282 for Iteration #15 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #15 of Tilt-Series #21. The scaled sum of CCMS values is #0.02788 for iteration 15 of Tilt-Series #21. Alignment quality for Iteration #15 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #15 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #15 in Parallel... Creating Refinement tilt-series video for iteration #15... Generating Refinement reconstruction for iteration #15... The tilt azimuth after iteration #15 deviated from the initial tilt azimuth by 2.655000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #16 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #16, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #16 Creating quality assessment statistics... CCMS(shift) = 0.02979 for Iteration #16 of Tilt-Series #21. CCMS(rotations) = 0.72531 for Iteration #16 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #16 of Tilt-Series #21. The scaled sum of CCMS values is #0.0294 for iteration 16 of Tilt-Series #21. Alignment quality for Iteration #16 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #16 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #16 in Parallel... Creating Refinement tilt-series video for iteration #16... Generating Refinement reconstruction for iteration #16... The tilt azimuth after iteration #16 deviated from the initial tilt azimuth by 1.909000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #17 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #17, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #17 Creating quality assessment statistics... CCMS(shift) = 0.02149 for Iteration #17 of Tilt-Series #21. CCMS(rotations) = 0.82557 for Iteration #17 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #17 of Tilt-Series #21. The scaled sum of CCMS values is #0.02726 for iteration 17 of Tilt-Series #21. Alignment quality for Iteration #17 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #17 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #17 in Parallel... Creating Refinement tilt-series video for iteration #17... Generating Refinement reconstruction for iteration #17... The tilt azimuth after iteration #17 deviated from the initial tilt azimuth by 2.526000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #18 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #18, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #18 Creating quality assessment statistics... CCMS(shift) = 0.03108 for Iteration #18 of Tilt-Series #21. CCMS(rotations) = 0.8183 for Iteration #18 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #18 of Tilt-Series #21. The scaled sum of CCMS values is #0.0319 for iteration 18 of Tilt-Series #21. Alignment quality for Iteration #18 of Tilt-Series #21 (19feb06g): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #18 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #18 in Parallel... Creating Refinement tilt-series video for iteration #18... Generating Refinement reconstruction for iteration #18... The tilt azimuth after iteration #18 deviated from the initial tilt azimuth by 2.061000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #19 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #19, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #19 Creating quality assessment statistics... CCMS(shift) = 0.021 for Iteration #19 of Tilt-Series #21. CCMS(rotations) = 0.91428 for Iteration #19 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #19 of Tilt-Series #21. The scaled sum of CCMS values is #0.02879 for iteration 19 of Tilt-Series #21. Alignment quality for Iteration #19 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #19 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #19 in Parallel... Creating Refinement tilt-series video for iteration #19... Generating Refinement reconstruction for iteration #19... The tilt azimuth after iteration #19 deviated from the initial tilt azimuth by 2.796000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #20 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #20, Round #2 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #20 Creating quality assessment statistics... CCMS(shift) = 0.03183 for Iteration #20 of Tilt-Series #21. CCMS(rotations) = 0.86198 for Iteration #20 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #20 of Tilt-Series #21. The scaled sum of CCMS values is #0.03316 for iteration 20 of Tilt-Series #21. Alignment quality for Iteration #20 of Tilt-Series #21 (19feb06g): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #20 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #20 in Parallel... Creating Refinement tilt-series video for iteration #20... Generating Refinement reconstruction for iteration #20... The tilt azimuth after iteration #20 deviated from the initial tilt azimuth by 2.066000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #21, Round #3 of Tilt-Series #21 (tiltseries0021_thick1800) lowpass = 22.4 angstroms Round #3. Parameters in Protomo units: (Note: At binned by 2, pixelsize is 5.595 Angstroms, Nyquist is 11.19 Angstroms) window.lowpass.apodization = { 0.199821428571 0.199821428571 } align.translimit = 150.5e0 map.sampling = 2 window.highpass.diameter = { 0.00145513654096 0.00145513654096 } window.highpass.apodization = { 0.00291027308192 0.00291027308192 } window.mask.apodization = { 10 10 } align.mask.width = { 1349 1483 } sampling = 2 align.mask.apodization = { 10 10 } map.body = 612.103630957 map.lowpass.diameter = { 0.499553571429 0.499553571429 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 1374 1508 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 1349 1483 } reference.body = 612.103630957 window.lowpass.diameter = { 0.499553571429 0.499553571429 } align.correlation.mode = mcf No parameters were switched for Iteration #21 Creating quality assessment statistics... CCMS(shift) = 0.02625 for Iteration #21 of Tilt-Series #21. CCMS(rotations) = 0.93206 for Iteration #21 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #21 of Tilt-Series #21. The scaled sum of CCMS values is #0.03177 for iteration 21 of Tilt-Series #21. Alignment quality for Iteration #21 of Tilt-Series #21 (19feb06g): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #21 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #21 in Parallel... Creating Refinement tilt-series video for iteration #21... Generating Refinement reconstruction for iteration #21... The tilt azimuth after iteration #21 deviated from the initial tilt azimuth by 3.129000 degrees, which is greater than the maximum allowed deviation of 2.500000 degrees.Changing the tilt azimuth back to the initial tilt azimuth of -80.000000 degrees for the next iteration. No Round parameters changed for Iteration #22 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #22, Round #3 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #22 Creating quality assessment statistics... CCMS(shift) = 0.02812 for Iteration #22 of Tilt-Series #21. CCMS(rotations) = 0.69268 for Iteration #22 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #22 of Tilt-Series #21. The scaled sum of CCMS values is #0.02791 for iteration 22 of Tilt-Series #21. Alignment quality for Iteration #22 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #22 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #22 in Parallel... Creating Refinement tilt-series video for iteration #22... Generating Refinement reconstruction for iteration #22... The tilt azimuth after iteration #22 deviated from the initial tilt azimuth by 1.808000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #23 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #23, Round #3 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #23 Creating quality assessment statistics... CCMS(shift) = 0.02139 for Iteration #23 of Tilt-Series #21. CCMS(rotations) = 0.68756 for Iteration #23 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #23 of Tilt-Series #21. The scaled sum of CCMS values is #0.02445 for iteration 23 of Tilt-Series #21. Alignment quality for Iteration #23 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #23 of Tilt-Series #21 (19feb06g): StableCreating Depiction Videos for Iteration #23 in Parallel... Creating Refinement tilt-series video for iteration #23... Generating Refinement reconstruction for iteration #23... The tilt azimuth after iteration #23 deviated from the initial tilt azimuth by 1.992000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #24 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #24, Round #3 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #24 Creating quality assessment statistics... CCMS(shift) = 0.01915 for Iteration #24 of Tilt-Series #21. CCMS(rotations) = 0.73995 for Iteration #24 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #24 of Tilt-Series #21. The scaled sum of CCMS values is #0.02437 for iteration 24 of Tilt-Series #21. Alignment quality for Iteration #24 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #24 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #24 in Parallel... Creating Refinement tilt-series video for iteration #24... Generating Refinement reconstruction for iteration #24... The tilt azimuth after iteration #24 deviated from the initial tilt azimuth by 1.931000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #25 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #25, Round #3 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #25 Creating quality assessment statistics... CCMS(shift) = 0.01576 for Iteration #25 of Tilt-Series #21. CCMS(rotations) = 0.76756 for Iteration #25 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #25 of Tilt-Series #21. The scaled sum of CCMS values is #0.02323 for iteration 25 of Tilt-Series #21. Alignment quality for Iteration #25 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #25 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #25 in Parallel... Creating Refinement tilt-series video for iteration #25... Generating Refinement reconstruction for iteration #25... The tilt azimuth after iteration #25 deviated from the initial tilt azimuth by 2.165000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #26, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) lowpass = 11.2 angstroms Round #4. Parameters in Protomo units: (Note: At binned by 1, pixelsize is 2.7975 Angstroms, Nyquist is 5.595 Angstroms) window.lowpass.apodization = { 0.199821428571 0.199821428571 } align.translimit = 301.0e0 map.sampling = 1 window.highpass.diameter = { 0.000727568270481 0.000727568270481 } window.highpass.apodization = { 0.00145513654096 0.00145513654096 } window.mask.apodization = { 10 10 } align.mask.width = { 2724 2992 } sampling = 1 align.mask.apodization = { 10 10 } map.body = 1224.20726191 map.lowpass.diameter = { 0.499553571429 0.499553571429 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 2749 3017 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 2724 2992 } reference.body = 1224.20726191 window.lowpass.diameter = { 0.499553571429 0.499553571429 } align.correlation.mode = mcf No parameters were switched for Iteration #26 Creating quality assessment statistics... CCMS(shift) = 0.01504 for Iteration #26 of Tilt-Series #21. CCMS(rotations) = 0.70257 for Iteration #26 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #26 of Tilt-Series #21. The scaled sum of CCMS values is #0.02157 for iteration 26 of Tilt-Series #21. Alignment quality for Iteration #26 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #26 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #26 in Parallel... Creating Refinement tilt-series video for iteration #26... Generating Refinement reconstruction for iteration #26... The tilt azimuth after iteration #26 deviated from the initial tilt azimuth by 1.979000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #27 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #27, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #27 Creating quality assessment statistics... CCMS(shift) = 0.01407 for Iteration #27 of Tilt-Series #21. CCMS(rotations) = 0.69632 for Iteration #27 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #27 of Tilt-Series #21. The scaled sum of CCMS values is #0.02096 for iteration 27 of Tilt-Series #21. Alignment quality for Iteration #27 of Tilt-Series #21 (19feb06g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #27 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #27 in Parallel... Creating Refinement tilt-series video for iteration #27... Generating Refinement reconstruction for iteration #27... The tilt azimuth after iteration #27 deviated from the initial tilt azimuth by 1.629000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #28 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #28, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #28 Creating quality assessment statistics... CCMS(shift) = 0.01384 for Iteration #28 of Tilt-Series #21. CCMS(rotations) = 0.5947 for Iteration #28 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #28 of Tilt-Series #21. The scaled sum of CCMS values is #0.01882 for iteration 28 of Tilt-Series #21. Alignment quality for Iteration #28 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #28 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #28 in Parallel... Creating Refinement tilt-series video for iteration #28... Generating Refinement reconstruction for iteration #28... The tilt azimuth after iteration #28 deviated from the initial tilt azimuth by 1.155000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #29 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #29, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #29 Creating quality assessment statistics... CCMS(shift) = 0.01529 for Iteration #29 of Tilt-Series #21. CCMS(rotations) = 0.51149 for Iteration #29 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #29 of Tilt-Series #21. The scaled sum of CCMS values is #0.01788 for iteration 29 of Tilt-Series #21. Alignment quality for Iteration #29 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #29 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #29 in Parallel... Creating Refinement tilt-series video for iteration #29... Generating Refinement reconstruction for iteration #29... The tilt azimuth after iteration #29 deviated from the initial tilt azimuth by 0.881000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #30 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #30, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #30 Creating quality assessment statistics... CCMS(shift) = 0.01071 for Iteration #30 of Tilt-Series #21. CCMS(rotations) = 0.52762 for Iteration #30 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #30 of Tilt-Series #21. The scaled sum of CCMS values is #0.01591 for iteration 30 of Tilt-Series #21. Alignment quality for Iteration #30 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #30 of Tilt-Series #21 (19feb06g): Rock Solid!Creating Depiction Videos for Iteration #30 in Parallel... Creating Refinement tilt-series video for iteration #30... Generating Refinement reconstruction for iteration #30... The tilt azimuth after iteration #30 deviated from the initial tilt azimuth by 0.650000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #31 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #31, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) fit.scale switched from false to true on Iteration #31Creating quality assessment statistics... CCMS(shift) = 0.01564 for Iteration #31 of Tilt-Series #21. CCMS(rotations) = 0.56638 for Iteration #31 of Tilt-Series #21. CCMS(scale) = 0.0 for Iteration #31 of Tilt-Series #21. The scaled sum of CCMS values is #0.01915 for iteration 31 of Tilt-Series #21. Alignment quality for Iteration #31 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #31 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #31 in Parallel... Creating Refinement tilt-series video for iteration #31... Generating Refinement reconstruction for iteration #31... The tilt azimuth after iteration #31 deviated from the initial tilt azimuth by 0.734000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #32 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #32, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #32 Creating quality assessment statistics... CCMS(shift) = 0.01336 for Iteration #32 of Tilt-Series #21. CCMS(rotations) = 0.55745 for Iteration #32 of Tilt-Series #21. CCMS(scale) = 0.00499 for Iteration #32 of Tilt-Series #21. The scaled sum of CCMS values is #0.01355 for iteration 32 of Tilt-Series #21. Alignment quality for Iteration #32 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #32 of Tilt-Series #21 (19feb06g): Very StableCreating Depiction Videos for Iteration #32 in Parallel... Creating Refinement tilt-series video for iteration #32... Generating Refinement reconstruction for iteration #32... The tilt azimuth after iteration #32 deviated from the initial tilt azimuth by 0.340000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #33 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #33, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #33 Creating quality assessment statistics... CCMS(shift) = 0.01655 for Iteration #33 of Tilt-Series #21. CCMS(rotations) = 0.4781 for Iteration #33 of Tilt-Series #21. CCMS(scale) = 0.00577 for Iteration #33 of Tilt-Series #21. The scaled sum of CCMS values is #0.01381 for iteration 33 of Tilt-Series #21. Alignment quality for Iteration #33 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #33 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #33 in Parallel... Creating Refinement tilt-series video for iteration #33... Generating Refinement reconstruction for iteration #33... The tilt azimuth after iteration #33 deviated from the initial tilt azimuth by 0.051000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #34 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #34, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #34 Creating quality assessment statistics... CCMS(shift) = 0.01517 for Iteration #34 of Tilt-Series #21. CCMS(rotations) = 0.5741 for Iteration #34 of Tilt-Series #21. CCMS(scale) = 0.00706 for Iteration #34 of Tilt-Series #21. The scaled sum of CCMS values is #0.01507 for iteration 34 of Tilt-Series #21. Alignment quality for Iteration #34 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #34 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #34 in Parallel... Creating Refinement tilt-series video for iteration #34... Generating Refinement reconstruction for iteration #34... The tilt azimuth after iteration #34 deviated from the initial tilt azimuth by 0.433000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #35 of Tilt-Series #21 (tiltseries0021_thick1800) Beginning Refinement Iteration #35, Round #4 of Tilt-Series #21 (tiltseries0021_thick1800) No parameters were switched for Iteration #35 Creating quality assessment statistics... CCMS(shift) = 0.01694 for Iteration #35 of Tilt-Series #21. CCMS(rotations) = 0.56965 for Iteration #35 of Tilt-Series #21. CCMS(scale) = 0.00763 for Iteration #35 of Tilt-Series #21. The scaled sum of CCMS values is #0.01579 for iteration 35 of Tilt-Series #21. Alignment quality for Iteration #35 of Tilt-Series #21 (19feb06g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #35 of Tilt-Series #21 (19feb06g): UnstableCreating Depiction Videos for Iteration #35 in Parallel... Creating Refinement tilt-series video for iteration #35... Generating Refinement reconstruction for iteration #35... The tilt azimuth after iteration #35 deviated from the initial tilt azimuth by 1.141000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees.Finished Refinement for Tilt-Series #21 (tiltseries0021_thick1800)! Did everything blow up and now you're yelling at your computer screen? If so, kindly post your issue on the following Appion-Protomo Google group or email Alex at anoble@nysbc.org explaining the issue and include this log file. https://groups.google.com/forum/#!topic/appion-protomo If everything worked beautifully and you publish, please use the appropriate citations listed on the Appion webpage! You can also print out all citations by typing: protomo2aligner.py --citations End time: 2019yr02m14d-04hr30m46s