DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0022/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0022/defocus_estimation/series0022_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0022/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 32087.9, 48131.82, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 49.1 48000.0 41000.0 37500.0 1 46.8 32000.0 40500.0 32500.0 2 44.5 41500.0 36000.0 47500.0 3 42.2 43000.0 33000.0 46000.0 4 39.9 42500.0 33000.0 48000.0 5 37.6 34000.0 33000.0 36000.0 6 35.3 45500.0 33000.0 37000.0 7 33.0 41000.0 37500.0 41000.0 8 30.7 34000.0 33500.0 34000.0 9 28.4 37000.0 37000.0 35000.0 10 26.1 32000.0 33500.0 43000.0 11 23.8 42500.0 33000.0 48000.0 12 21.5 33500.0 36500.0 45000.0 13 19.2 32000.0 34500.0 40000.0 14 16.9 35500.0 32500.0 34000.0 15 14.6 43500.0 40000.0 37000.0 16 12.3 44500.0 45000.0 32000.0 17 10.0 33000.0 33500.0 32500.0 18 10.0 33500.0 38000.0 32500.0 19 7.7 34000.0 35000.0 35000.0 20 5.4 36500.0 36000.0 32500.0 21 3.1 34500.0 34500.0 32000.0 22 0.8 33500.0 32000.0 35500.0 23 -1.5 34000.0 37500.0 39000.0 24 -3.8 35000.0 43500.0 36500.0 25 -6.1 32000.0 35500.0 48000.0 26 -8.4 38500.0 42500.0 33500.0 27 -10.7 33000.0 32000.0 34500.0 28 -13.0 39500.0 37500.0 38000.0 29 -15.3 33500.0 34500.0 34500.0 30 -17.6 34500.0 32500.0 46500.0 31 -19.9 32500.0 42500.0 47500.0 32 -22.2 37500.0 34000.0 32000.0 33 -24.5 40000.0 33000.0 34500.0 34 -26.8 43000.0 35500.0 42500.0 35 -29.1 34000.0 35500.0 43000.0 36 -31.4 36500.0 42000.0 46000.0 37 -33.7 35000.0 34000.0 38500.0 38 -36.0 32000.0 34500.0 43000.0 39 -38.3 35500.0 35500.0 46000.0 40 -40.6 35500.0 33500.0 39000.0 41 -42.9 33000.0 43500.0 38500.0 42 -45.2 34500.0 35500.0 47000.0 43 -47.5 38500.0 36500.0 43000.0 44 -49.8 34500.0 36500.0 41000.0 45 -52.1 32000.0 39000.0 43000.0 46 -54.4 34500.0 38000.0 34500.0 47 -56.7 39500.0 42500.0 41500.0 48 -59.0 37500.0 48000.0 33000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 119.130u 1.364s 2:11.99 91.2% 0+0k 0+0io 0pf+0w