DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0023/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0023/defocus_estimation/series0023_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19feb06g/protomo_alignments/tiltseries0023/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 2.8 Angstroms/Pixel: 2.80 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 11.2 dFMin[A], dFMax[A], FStep: 31623.4, 47435.14, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 46.8 31500.0 31500.0 44000.0 1 44.5 38500.0 33500.0 42500.0 2 42.2 41500.0 34500.0 31500.0 3 39.9 40500.0 42500.0 45500.0 4 37.6 35500.0 43500.0 47000.0 5 35.3 47000.0 34500.0 39500.0 6 33.0 36000.0 40500.0 47000.0 7 30.7 31500.0 38000.0 37500.0 8 28.4 43500.0 39500.0 45000.0 9 26.1 32500.0 31500.0 31500.0 10 23.8 40500.0 40500.0 38500.0 11 21.5 32000.0 33000.0 34000.0 12 19.2 40500.0 31500.0 40000.0 13 16.9 40500.0 39500.0 42000.0 14 14.6 34500.0 38500.0 34500.0 15 12.3 32500.0 32000.0 39000.0 16 10.0 46000.0 42000.0 34000.0 17 10.0 42500.0 32500.0 36500.0 18 7.7 44000.0 38000.0 40500.0 19 5.4 38500.0 32000.0 42500.0 20 3.1 38500.0 31500.0 36500.0 21 0.8 38000.0 32500.0 37500.0 22 -1.5 42500.0 42000.0 44500.0 23 -3.8 31500.0 31500.0 38000.0 24 -6.1 47000.0 34500.0 39500.0 25 -8.4 38000.0 43000.0 34000.0 26 -10.7 44000.0 36000.0 33500.0 27 -13.0 38000.0 36500.0 32000.0 28 -15.3 37000.0 45500.0 31500.0 29 -17.6 32500.0 37500.0 40000.0 30 -19.9 31500.0 33500.0 36000.0 31 -22.2 40000.0 33000.0 35500.0 32 -24.5 39500.0 38500.0 39500.0 33 -26.8 38500.0 42000.0 32000.0 34 -29.1 39500.0 38000.0 31500.0 35 -31.4 32000.0 40000.0 37000.0 36 -33.7 32000.0 41500.0 32000.0 37 -36.0 39000.0 33000.0 33500.0 38 -38.3 31500.0 32000.0 42000.0 39 -40.6 39500.0 40500.0 39500.0 40 -42.9 38000.0 41000.0 33000.0 41 -45.2 38500.0 35500.0 35000.0 42 -47.5 35000.0 33000.0 43000.0 43 -49.8 42000.0 46000.0 43500.0 44 -52.1 31500.0 40500.0 34000.0 45 -54.4 36000.0 37500.0 33000.0 46 -56.7 43500.0 35000.0 44500.0 47 -59.0 32500.0 36000.0 40000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 102.339u 1.152s 1:43.63 99.8% 0+0k 0+0io 0pf+0w