protomo2aligner.py --my_tlt=False --r5_kernel_x=5 --r5_iters=0 --r4_mask_apod_y=10 --r4_mask_apod_x=10 --corr_mode=mcf --rundir=/gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0010_thick1200_thick1000 --r1_peak_search_radius_y=300.0 --r1_peak_search_radius_x=300.0 --serialem_stack= --r3_highpass_diameter_x=3861.0 --r4_lowpass_diameter_x=3.68 --filename_prefix= --r4_cmdiameter_x=100.0 --r4_cmdiameter_y=100.0 --r1_region_x=2103 --r1_region_y=3096 --cmdiameter_y=100.0 --r2_corr_mode=mcf --r5_highpass_diameter_x=3861.0 --r4_lowpass_apod_y=9.0 --r4_lowpass_apod_x=9.0 --r3_kernel_y=5 --r3_kernel_x=5 --r3_lowpass_apod_x=9.0 --r3_lowpass_apod_y=9.0 --manual_alignment_finished=False --r5_body=0 --runname=tiltseries0010_thick1200_thick1000 --r2_region_y=3096 --r2_region_x=2103 --r2_sampling=1 --video_type=html5vid --rotation=true --r2_highpass_apod_y=1931.0 --defocus_ang_positive=90 --r5_mask_width_x=2078 --frame_aligned=True --image_file_type=mrc --r3_highpass_diameter_y=3861.0 --r3_highpass_apod_x=1931.0 --r5_cmdiameter_y=100.0 --r5_cmdiameter_x=100.0 --r2_mask_width_x=2078 --r2_mask_width_y=3071 --r5_region_x=2103 --r5_region_y=3096 --highpass_diameter_y=0.001 --highpass_diameter_x=0.001 --create_reconstruction=true --r5_mask_apod_x=10 --r5_mask_apod_y=10 --r4_lowpass_diameter_y=3.68 --r1_body=0 --r1_mask_apod_x=10 --defocus_difference=0.2 --correlation_size_y=160 --correlation_size_x=160 --r2_cmdiameter_x=100.0 --r2_cmdiameter_y=100.0 --gridsearch_limit=2.0 --azimuth_stability_check=True --r1_highpass_diameter_y=3861.0 --r4_kernel_x=5 --r4_kernel_y=5 --amp_contrast_defocus=0.07 --peak_search_radius_x=100.0 --peak_search_radius_y=100.0 --r5_mask_width_y=3071 --window_area=0.95 --r1_corr_mode=mcf --gradient=true --create_tilt_video=true --mask_apod_y=10 --r5_peak_search_radius_y=300.0 --exclude_images_by_angle= --r3_mask_apod_x=10 --r3_mask_apod_y=10 --norotations=false --r3_highpass_apod_y=1931.0 --r3_iters=0 --r1_highpass_diameter_x=3861.0 --r2_lowpass_diameter_y=7.36 --map_size_y=463 --map_size_z=200 --r5_lowpass_diameter_y=3.68 --r5_lowpass_diameter_x=3.68 --max_shift=999999 --cachedir=cache --clip_high=3.5 --grow=0 --r3_cmdiameter_y=100.0 --r3_cmdiameter_x=100.0 --keep_recons=false --r4_mask_width_x=2078 --r4_mask_width_y=3071 --r5_highpass_apod_y=1931.0 --logging=true --DefocusTol=200 --filter=median --sessionname=18nov10g --exclude_images=999999 --commit=False --r1_kernel_y=5 --r1_kernel_x=5 --dose_presets=False --mask_apod_x=10 --r4_corr_mode=mcf --show_window_size=true --defocus_estimate=False --r4_iters=0 --amp_contrast_ctf=0.07 --r1_highpass_apod_y=1931.0 --r1_highpass_apod_x=1931.0 --r2_highpass_apod_x=1931.0 --sampling=8 --starting_tlt_file=Manual --r5_lowpass_apod_x=9.0 --r5_lowpass_apod_y=9.0 --dose_c=2.81 --r1_cmdiameter_y=100.0 --further_alignment=False --r1_lowpass_diameter_y=14.72 --r1_lowpass_diameter_x=14.72 --r4_sampling=1 --res_max=10 --r1_mask_apod_y=10 --res_min=10000 --scale_switch=16 --dose_b=-1.665 --positive=90.0 --r3_peak_search_radius_y=300.0 --r3_peak_search_radius_x=300.0 --negative=-90.0 --parallel=True --tiltseries=10 --r5_corr_mode=pcf --change_refimg=False --r3_mask_width_y=3071 --r3_mask_width_x=2078 --map_sampling=8 --thr_low=0 --gridsearch_step=0.1 --coarse=False --r2_iters=10 --region_y=512 --r4_highpass_diameter_y=3861.0 --r4_highpass_diameter_x=3861.0 --mask_width_x=1024 --highpass_apod_x=0.002 --binning=true --r1_lowpass_apod_y=37.0 --thr_high=999999 --r5_sampling=1 --highpass_apod_y=0.002 --defocus_min=0 --r1_sampling=2 --ctf_correct=False --r2_highpass_diameter_y=3861.0 --r2_mask_apod_y=10 --r2_mask_apod_x=10 --desired_ref_tilt_angle=0.0 --r5_highpass_apod_x=1931.0 --defocus_save=0 --protomo_outdir=out --r5_highpass_diameter_y=3861.0 --defocus_max=0 --r4_region_y=3096 --r4_region_x=2103 --r5_kernel_y=5 --r3_region_x=2103 --r3_region_y=3096 --r4_body=0 --serialem_mdoc= --r2_peak_search_radius_x=300.0 --r2_peak_search_radius_y=300.0 --thickness=1000 --expid=7953 --r2_body=0 --orientation=true --do_estimation=false --iter_gradient=false --projectid=282 --map_size_x=479 --r3_body=0 --r3_lowpass_diameter_y=3.68 --r3_lowpass_diameter_x=3.68 --mask_width_y=1024 --max_correction=999999 --elevation=false --r2_kernel_x=5 --r2_kernel_y=5 --slab=true --pixelsize=1.83586 --link=False --r2_highpass_diameter_x=3861.0 --lowpass_diameter_x=0.5 --lowpass_diameter_y=0.5 --defocus=3 --border=100 --r3_corr_mode=mcf --r2_lowpass_apod_y=18.0 --exclude_images_by_angle_accuracy=0.05 --r2_lowpass_apod_x=18.0 --defocus_ang_negative=-90 --r3_sampling=1 --description= --make_searchable=True --translimit=301.0 --iters=1 --r5_peak_search_radius_x=300.0 --r1_iters=10 --r1_cmdiameter_x=100.0 --preprocessing=true --scale=false --iWidth=20 --r2_lowpass_diameter_x=7.36 --dimx=3838 --dimy=3710 --r4_peak_search_radius_x=300.0 --r4_peak_search_radius_y=300.0 --r1_lowpass_apod_x=37.0 --region_x=512 --manual_alignment=False --loglevel=2 --restart_cycle=0 --dose_a=0.245 --lowpass_apod_y=0.05 --lowpass_apod_x=0.05 --kernel_x=3 --kernel_y=3 --clip_low=3.5 --azimuth_max_deviation=2.5 --tilt_clip=true --r4_highpass_apod_x=1931.0 --r4_highpass_apod_y=1931.0 --azimuth=true --r1_mask_width_y=1523 --r1_mask_width_x=1026 --jobtype=protomo --from_multirefine=True node39 Start time: 2019yr05m28d-10hr39m35s Description: Using alignment .tlt file as determined by protomo2multirefine.py.Starting Protomo alignment Beginning Refinements Beginning Refinement Iteration #1, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) lowpass = 14.72 Angstroms Round #1. Parameters in Protomo units: (Note: At binned by 2, pixelsize is 3.67172 Angstroms, Nyquist is 7.34344 Angstroms) window.lowpass.apodization = { 0.198471351351 0.198471351351 } align.translimit = 150.5e0 map.sampling = 2 window.highpass.diameter = { 0.00190195286195 0.00190195286195 } window.highpass.apodization = { 0.00380292076644 0.00380292076644 } window.mask.apodization = { 10 10 } align.mask.width = { 1026 1523 } sampling = 2 align.mask.apodization = { 10 10 } map.body = 393.447766657 map.lowpass.diameter = { 0.498875 0.498875 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 1051 1548 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 1026 1523 } reference.body = 393.447766657 window.lowpass.diameter = { 0.498875 0.498875 } align.correlation.mode = mcf No parameters were switched for Iteration #1 Creating quality assessment statistics... CCMS(shift) = 0.02947 for Iteration #1 of Tilt-Series #10. CCMS(rotations) = 1.09017 for Iteration #1 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #1 of Tilt-Series #10. The scaled sum of CCMS values is #0.03654 for iteration 1 of Tilt-Series #10. Alignment quality for Iteration #1 of Tilt-Series #10 (18nov10g): Bad - 1st iteration is always bad because the geometry has not yet been estimated (confidence: 100.000000%)Creating Depiction Videos for Iteration #1 in Parallel... Creating Refinement tilt-series video for iteration #1... Generating Refinement reconstruction for iteration #1... The tilt azimuth after iteration #1 deviated from the initial tilt azimuth by 0.652000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #2, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #2 Creating quality assessment statistics... CCMS(shift) = 0.03523 for Iteration #2 of Tilt-Series #10. CCMS(rotations) = 0.96023 for Iteration #2 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #2 of Tilt-Series #10. The scaled sum of CCMS values is #0.03682 for iteration 2 of Tilt-Series #10. Alignment quality for Iteration #2 of Tilt-Series #10 (18nov10g): Bad (confidence: 100.000000%)Creating Depiction Videos for Iteration #2 in Parallel... Creating Refinement tilt-series video for iteration #2... Generating Refinement reconstruction for iteration #2... The tilt azimuth after iteration #2 deviated from the initial tilt azimuth by 1.822000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #3, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #3 Creating quality assessment statistics... CCMS(shift) = 0.02792 for Iteration #3 of Tilt-Series #10. CCMS(rotations) = 0.7517 for Iteration #3 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #3 of Tilt-Series #10. The scaled sum of CCMS values is #0.029 for iteration 3 of Tilt-Series #10. Alignment quality for Iteration #3 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Creating Depiction Videos for Iteration #3 in Parallel... Creating Refinement tilt-series video for iteration #3... Generating Refinement reconstruction for iteration #3... The tilt azimuth after iteration #3 deviated from the initial tilt azimuth by 1.270000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #4 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #4, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #4 Creating quality assessment statistics... CCMS(shift) = 0.02758 for Iteration #4 of Tilt-Series #10. CCMS(rotations) = 0.77807 for Iteration #4 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #4 of Tilt-Series #10. The scaled sum of CCMS values is #0.02935 for iteration 4 of Tilt-Series #10. Alignment quality for Iteration #4 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #4 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #4 in Parallel... Creating Refinement tilt-series video for iteration #4... Generating Refinement reconstruction for iteration #4... The tilt azimuth after iteration #4 deviated from the initial tilt azimuth by 1.476000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #5 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #5, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #5 Creating quality assessment statistics... CCMS(shift) = 0.02625 for Iteration #5 of Tilt-Series #10. CCMS(rotations) = 0.79203 for Iteration #5 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #5 of Tilt-Series #10. The scaled sum of CCMS values is #0.02897 for iteration 5 of Tilt-Series #10. Alignment quality for Iteration #5 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #5 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #5 in Parallel... Creating Refinement tilt-series video for iteration #5... Generating Refinement reconstruction for iteration #5... The tilt azimuth after iteration #5 deviated from the initial tilt azimuth by 0.811000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #6 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #6, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #6 Creating quality assessment statistics... CCMS(shift) = 0.03663 for Iteration #6 of Tilt-Series #10. CCMS(rotations) = 0.9031 for Iteration #6 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #6 of Tilt-Series #10. The scaled sum of CCMS values is #0.03638 for iteration 6 of Tilt-Series #10. Alignment quality for Iteration #6 of Tilt-Series #10 (18nov10g): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #6 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #6 in Parallel... Creating Refinement tilt-series video for iteration #6... Generating Refinement reconstruction for iteration #6... The tilt azimuth after iteration #6 deviated from the initial tilt azimuth by 0.806000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #7 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #7, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #7 Creating quality assessment statistics... CCMS(shift) = 0.03093 for Iteration #7 of Tilt-Series #10. CCMS(rotations) = 0.54877 for Iteration #7 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #7 of Tilt-Series #10. The scaled sum of CCMS values is #0.02644 for iteration 7 of Tilt-Series #10. Alignment quality for Iteration #7 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #7 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #7 in Parallel... Creating Refinement tilt-series video for iteration #7... Generating Refinement reconstruction for iteration #7... The tilt azimuth after iteration #7 deviated from the initial tilt azimuth by 0.814000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #8 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #8, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #8 Creating quality assessment statistics... CCMS(shift) = 0.02916 for Iteration #8 of Tilt-Series #10. CCMS(rotations) = 0.68221 for Iteration #8 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #8 of Tilt-Series #10. The scaled sum of CCMS values is #0.02822 for iteration 8 of Tilt-Series #10. Alignment quality for Iteration #8 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #8 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #8 in Parallel... Creating Refinement tilt-series video for iteration #8... Generating Refinement reconstruction for iteration #8... The tilt azimuth after iteration #8 deviated from the initial tilt azimuth by 1.691000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #9 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #9, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #9 Creating quality assessment statistics... CCMS(shift) = 0.02579 for Iteration #9 of Tilt-Series #10. CCMS(rotations) = 0.44884 for Iteration #9 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #9 of Tilt-Series #10. The scaled sum of CCMS values is #0.02187 for iteration 9 of Tilt-Series #10. Alignment quality for Iteration #9 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #9 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #9 in Parallel... Creating Refinement tilt-series video for iteration #9... Generating Refinement reconstruction for iteration #9... The tilt azimuth after iteration #9 deviated from the initial tilt azimuth by 1.564000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #10 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #10, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #10 Creating quality assessment statistics... CCMS(shift) = 0.02414 for Iteration #10 of Tilt-Series #10. CCMS(rotations) = 0.33291 for Iteration #10 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #10 of Tilt-Series #10. The scaled sum of CCMS values is #0.01873 for iteration 10 of Tilt-Series #10. Alignment quality for Iteration #10 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #10 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #10 in Parallel... Creating Refinement tilt-series video for iteration #10... Generating Refinement reconstruction for iteration #10... The tilt azimuth after iteration #10 deviated from the initial tilt azimuth by 1.652000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #11, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) lowpass = 7.36 angstroms Round #2. Parameters in Protomo units: (Note: At binned by 1, pixelsize is 1.83586 Angstroms, Nyquist is 3.67172 Angstroms) window.lowpass.apodization = { 0.203984444444 0.203984444444 } align.translimit = 301.0e0 map.sampling = 1 window.highpass.diameter = { 0.000950976430976 0.000950976430976 } window.highpass.apodization = { 0.00190146038322 0.00190146038322 } window.mask.apodization = { 10 10 } align.mask.width = { 2078 3071 } sampling = 1 align.mask.apodization = { 10 10 } map.body = 786.895533313 map.lowpass.diameter = { 0.498875 0.498875 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 2103 3096 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 2078 3071 } reference.body = 786.895533313 window.lowpass.diameter = { 0.498875 0.498875 } align.correlation.mode = mcf No parameters were switched for Iteration #11 Creating quality assessment statistics... CCMS(shift) = 0.02674 for Iteration #11 of Tilt-Series #10. CCMS(rotations) = 0.39446 for Iteration #11 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #11 of Tilt-Series #10. The scaled sum of CCMS values is #0.02126 for iteration 11 of Tilt-Series #10. Alignment quality for Iteration #11 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #11 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #11 in Parallel... Creating Refinement tilt-series video for iteration #11... Generating Refinement reconstruction for iteration #11... The tilt azimuth after iteration #11 deviated from the initial tilt azimuth by 1.621000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #12 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #12, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #12 Creating quality assessment statistics... CCMS(shift) = 0.02854 for Iteration #12 of Tilt-Series #10. CCMS(rotations) = 0.42863 for Iteration #12 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #12 of Tilt-Series #10. The scaled sum of CCMS values is #0.02284 for iteration 12 of Tilt-Series #10. Alignment quality for Iteration #12 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #12 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #12 in Parallel... Creating Refinement tilt-series video for iteration #12... Generating Refinement reconstruction for iteration #12... The tilt azimuth after iteration #12 deviated from the initial tilt azimuth by 1.166000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #13 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #13, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #13 Creating quality assessment statistics... CCMS(shift) = 0.02332 for Iteration #13 of Tilt-Series #10. CCMS(rotations) = 0.47761 for Iteration #13 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #13 of Tilt-Series #10. The scaled sum of CCMS values is #0.02121 for iteration 13 of Tilt-Series #10. Alignment quality for Iteration #13 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #13 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #13 in Parallel... Creating Refinement tilt-series video for iteration #13... Generating Refinement reconstruction for iteration #13... The tilt azimuth after iteration #13 deviated from the initial tilt azimuth by 1.461000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #14 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #14, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #14 Creating quality assessment statistics... CCMS(shift) = 0.02503 for Iteration #14 of Tilt-Series #10. CCMS(rotations) = 0.45398 for Iteration #14 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #14 of Tilt-Series #10. The scaled sum of CCMS values is #0.0216 for iteration 14 of Tilt-Series #10. Alignment quality for Iteration #14 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #14 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #14 in Parallel... Creating Refinement tilt-series video for iteration #14... Generating Refinement reconstruction for iteration #14... The tilt azimuth after iteration #14 deviated from the initial tilt azimuth by 1.895000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #15 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #15, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #15 Creating quality assessment statistics... CCMS(shift) = 0.02705 for Iteration #15 of Tilt-Series #10. CCMS(rotations) = 0.40006 for Iteration #15 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #15 of Tilt-Series #10. The scaled sum of CCMS values is #0.02152 for iteration 15 of Tilt-Series #10. Alignment quality for Iteration #15 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #15 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #15 in Parallel... Creating Refinement tilt-series video for iteration #15... Generating Refinement reconstruction for iteration #15... The tilt azimuth after iteration #15 deviated from the initial tilt azimuth by 1.790000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #16 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #16, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) fit.scale switched from false to true on Iteration #16Creating quality assessment statistics... CCMS(shift) = 0.02464 for Iteration #16 of Tilt-Series #10. CCMS(rotations) = 0.50143 for Iteration #16 of Tilt-Series #10. CCMS(scale) = 0.0 for Iteration #16 of Tilt-Series #10. The scaled sum of CCMS values is #0.02235 for iteration 16 of Tilt-Series #10. Alignment quality for Iteration #16 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #16 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #16 in Parallel... Creating Refinement tilt-series video for iteration #16... Generating Refinement reconstruction for iteration #16... The tilt azimuth after iteration #16 deviated from the initial tilt azimuth by 1.687000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #17 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #17, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #17 Creating quality assessment statistics... CCMS(shift) = 0.02286 for Iteration #17 of Tilt-Series #10. CCMS(rotations) = 0.57169 for Iteration #17 of Tilt-Series #10. CCMS(scale) = 0.01118 for Iteration #17 of Tilt-Series #10. The scaled sum of CCMS values is #0.01897 for iteration 17 of Tilt-Series #10. Alignment quality for Iteration #17 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #17 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #17 in Parallel... Creating Refinement tilt-series video for iteration #17... Generating Refinement reconstruction for iteration #17... The tilt azimuth after iteration #17 deviated from the initial tilt azimuth by 1.482000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #18 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #18, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #18 Creating quality assessment statistics... CCMS(shift) = 0.02316 for Iteration #18 of Tilt-Series #10. CCMS(rotations) = 0.88128 for Iteration #18 of Tilt-Series #10. CCMS(scale) = 0.01071 for Iteration #18 of Tilt-Series #10. The scaled sum of CCMS values is #0.02304 for iteration 18 of Tilt-Series #10. Alignment quality for Iteration #18 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #18 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #18 in Parallel... Creating Refinement tilt-series video for iteration #18... Generating Refinement reconstruction for iteration #18... The tilt azimuth after iteration #18 deviated from the initial tilt azimuth by 1.326000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #19 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #19, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #19 Creating quality assessment statistics... CCMS(shift) = 0.03588 for Iteration #19 of Tilt-Series #10. CCMS(rotations) = 0.64381 for Iteration #19 of Tilt-Series #10. CCMS(scale) = 0.01197 for Iteration #19 of Tilt-Series #10. The scaled sum of CCMS values is #0.02454 for iteration 19 of Tilt-Series #10. Alignment quality for Iteration #19 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #19 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #19 in Parallel... Creating Refinement tilt-series video for iteration #19... Generating Refinement reconstruction for iteration #19... The tilt azimuth after iteration #19 deviated from the initial tilt azimuth by 1.758000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #20 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) Beginning Refinement Iteration #20, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1000) No parameters were switched for Iteration #20 Creating quality assessment statistics... CCMS(shift) = 0.0263 for Iteration #20 of Tilt-Series #10. CCMS(rotations) = 0.58942 for Iteration #20 of Tilt-Series #10. CCMS(scale) = 0.01519 for Iteration #20 of Tilt-Series #10. The scaled sum of CCMS values is #0.02169 for iteration 20 of Tilt-Series #10. Alignment quality for Iteration #20 of Tilt-Series #10 (18nov10g): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #20 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #20 in Parallel... Creating Refinement tilt-series video for iteration #20... Generating Refinement reconstruction for iteration #20... The tilt azimuth after iteration #20 deviated from the initial tilt azimuth by 1.452000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees.Finished Refinement for Tilt-Series #10 (tiltseries0010_thick1200_thick1000)! Did everything blow up and now you're yelling at your computer screen? If so, kindly post your issue on the following Appion-Protomo Google group or email Alex at anoble@nysbc.org explaining the issue and include this log file. https://groups.google.com/forum/#!topic/appion-protomo If everything worked beautifully and you publish, please use the appropriate citations listed on the Appion webpage! You can also print out all citations by typing: protomo2aligner.py --citations End time: 2019yr05m29d-12hr49m07s