DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0010/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0010/defocus_estimation/series0010_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0010/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 52.8 41500.0 37000.0 33000.0 1 50.6 41000.0 37500.0 33000.0 2 48.4 40000.0 37500.0 32000.0 3 46.2 40000.0 37000.0 33000.0 4 44.0 39500.0 37500.0 34000.0 5 41.8 39500.0 36000.0 34000.0 6 39.6 38500.0 36000.0 34000.0 7 37.4 38500.0 36000.0 33500.0 8 35.2 38000.0 36000.0 33000.0 9 33.0 36000.0 33000.0 32000.0 10 30.8 34500.0 33500.0 32500.0 11 28.6 34500.0 33500.0 33000.0 12 26.4 35500.0 33000.0 32000.0 13 24.2 34000.0 33000.0 32000.0 14 22.0 34500.0 33000.0 32000.0 15 19.8 34000.0 33000.0 32000.0 16 17.6 34500.0 33000.0 32500.0 17 15.4 34000.0 33000.0 33000.0 18 13.2 34500.0 34000.0 32500.0 19 11.0 34000.0 34500.0 33500.0 20 8.8 34000.0 33500.0 33500.0 21 6.6 34000.0 33500.0 33500.0 22 4.4 34000.0 34000.0 33000.0 23 2.2 34500.0 35000.0 34000.0 24 0.0 35000.0 34500.0 35000.0 25 -0.0 33000.0 33000.0 32500.0 26 -2.2 33000.0 32500.0 32500.0 27 -4.4 32000.0 32500.0 32500.0 28 -6.6 32000.0 32000.0 32000.0 29 -8.8 32000.0 32500.0 33000.0 30 -11.0 32000.0 32000.0 32500.0 31 -13.2 32000.0 32000.0 32500.0 32 -15.4 32000.0 32000.0 32500.0 33 -17.6 32000.0 32000.0 33000.0 34 -19.8 32000.0 33000.0 32500.0 35 -22.0 32000.0 32000.0 33000.0 36 -24.2 32000.0 32000.0 33000.0 37 -26.4 32000.0 32000.0 33500.0 38 -28.6 32000.0 32000.0 33000.0 39 -30.8 32000.0 32000.0 32000.0 40 -33.0 32500.0 32000.0 32500.0 41 -35.2 48000.0 32000.0 32500.0 42 -37.4 32000.0 32000.0 33500.0 43 -39.6 46500.0 32000.0 32500.0 44 -41.8 32000.0 33000.0 33000.0 45 -44.0 34500.0 32000.0 33500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 103.949u 1.056s 1:48.93 96.3% 0+0k 0+0io 0pf+0w