protomo2aligner.py --my_tlt=False --r5_kernel_x=5 --r5_iters=0 --r4_mask_apod_y=10 --r4_mask_apod_x=10 --corr_mode=mcf --rundir=/gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0010_thick1200_thick1200_thick1400 --r1_peak_search_radius_y=300.0 --r1_peak_search_radius_x=300.0 --serialem_stack= --r3_highpass_diameter_x=3820.0 --r4_lowpass_diameter_x=3.68 --filename_prefix= --r4_cmdiameter_x=100.0 --r4_cmdiameter_y=100.0 --r1_region_x=2081 --r1_region_y=3146 --cmdiameter_y=100.0 --r2_corr_mode=mcf --r5_highpass_diameter_x=3820.0 --r4_lowpass_apod_y=9.0 --r4_lowpass_apod_x=9.0 --r3_kernel_y=5 --r3_kernel_x=5 --r3_lowpass_apod_x=9.0 --r3_lowpass_apod_y=9.0 --manual_alignment_finished=False --r5_body=0 --runname=tiltseries0010_thick1200_thick1200_thick1400 --r2_region_y=3146 --r2_region_x=2081 --r2_sampling=1 --video_type=html5vid --rotation=true --r2_highpass_apod_y=1910.0 --defocus_ang_positive=90 --r5_mask_width_x=2056 --frame_aligned=True --image_file_type=mrc --r3_highpass_diameter_y=3820.0 --r3_highpass_apod_x=1910.0 --r5_cmdiameter_y=100.0 --r5_cmdiameter_x=100.0 --r2_mask_width_x=2056 --r2_mask_width_y=3121 --r5_region_x=2081 --r5_region_y=3146 --highpass_diameter_y=0.001 --highpass_diameter_x=0.001 --create_reconstruction=true --r5_mask_apod_x=10 --r5_mask_apod_y=10 --r4_lowpass_diameter_y=3.68 --r1_body=0 --r1_mask_apod_x=10 --defocus_difference=0.2 --correlation_size_y=160 --correlation_size_x=160 --r2_cmdiameter_x=100.0 --r2_cmdiameter_y=100.0 --gridsearch_limit=2.0 --azimuth_stability_check=True --r1_highpass_diameter_y=3820.0 --r4_kernel_x=5 --r4_kernel_y=5 --amp_contrast_defocus=0.07 --peak_search_radius_x=100.0 --peak_search_radius_y=100.0 --r5_mask_width_y=3121 --window_area=0.95 --r1_corr_mode=mcf --gradient=true --create_tilt_video=true --mask_apod_y=10 --r5_peak_search_radius_y=300.0 --exclude_images_by_angle= --r3_mask_apod_x=10 --r3_mask_apod_y=10 --norotations=false --r3_highpass_apod_y=1910.0 --r3_iters=5 --r1_highpass_diameter_x=3820.0 --r2_lowpass_diameter_y=7.36 --map_size_y=463 --map_size_z=200 --r5_lowpass_diameter_y=3.68 --r5_lowpass_diameter_x=3.68 --max_shift=999999 --cachedir=cache --clip_high=3.5 --grow=0 --r3_cmdiameter_y=100.0 --r3_cmdiameter_x=100.0 --keep_recons=false --r4_mask_width_x=2056 --r4_mask_width_y=3121 --r5_highpass_apod_y=1910.0 --logging=true --DefocusTol=200 --filter=median --sessionname=18nov10g --exclude_images=999999 --commit=False --r1_kernel_y=5 --r1_kernel_x=5 --dose_presets=False --mask_apod_x=10 --r4_corr_mode=mcf --show_window_size=true --defocus_estimate=False --r4_iters=0 --amp_contrast_ctf=0.07 --r1_highpass_apod_y=1910.0 --r1_highpass_apod_x=1910.0 --r2_highpass_apod_x=1910.0 --sampling=8 --starting_tlt_file=Manual --r5_lowpass_apod_x=9.0 --r5_lowpass_apod_y=9.0 --dose_c=2.81 --r1_cmdiameter_y=100.0 --further_alignment=False --r1_lowpass_diameter_y=14.72 --r1_lowpass_diameter_x=14.72 --r4_sampling=1 --res_max=10 --r1_mask_apod_y=10 --res_min=10000 --scale_switch=16 --dose_b=-1.665 --positive=90.0 --r3_peak_search_radius_y=300.0 --r3_peak_search_radius_x=300.0 --negative=-90.0 --parallel=True --tiltseries=10 --r5_corr_mode=pcf --change_refimg=False --r3_mask_width_y=3121 --r3_mask_width_x=2056 --map_sampling=8 --thr_low=0 --gridsearch_step=0.1 --coarse=False --r2_iters=10 --region_y=512 --r4_highpass_diameter_y=3820.0 --r4_highpass_diameter_x=3820.0 --mask_width_x=1024 --highpass_apod_x=0.002 --binning=true --r1_lowpass_apod_y=37.0 --thr_high=999999 --r5_sampling=1 --highpass_apod_y=0.002 --defocus_min=0 --r1_sampling=2 --ctf_correct=False --r2_highpass_diameter_y=3820.0 --r2_mask_apod_y=10 --r2_mask_apod_x=10 --desired_ref_tilt_angle=0.0 --r5_highpass_apod_x=1910.0 --defocus_save=0 --protomo_outdir=out --r5_highpass_diameter_y=3820.0 --defocus_max=0 --r4_region_y=3146 --r4_region_x=2081 --r5_kernel_y=5 --r3_region_x=2081 --r3_region_y=3146 --r4_body=0 --serialem_mdoc= --r2_peak_search_radius_x=300.0 --r2_peak_search_radius_y=300.0 --thickness=1400 --expid=7953 --r2_body=0 --orientation=true --do_estimation=false --iter_gradient=false --projectid=282 --map_size_x=479 --r3_body=0 --r3_lowpass_diameter_y=3.68 --r3_lowpass_diameter_x=3.68 --mask_width_y=1024 --max_correction=999999 --elevation=false --r2_kernel_x=5 --r2_kernel_y=5 --slab=true --pixelsize=1.83586 --link=False --r2_highpass_diameter_x=3820.0 --lowpass_diameter_x=0.5 --lowpass_diameter_y=0.5 --defocus=3 --border=100 --r3_corr_mode=mcf --r2_lowpass_apod_y=18.0 --exclude_images_by_angle_accuracy=0.05 --r2_lowpass_apod_x=18.0 --defocus_ang_negative=-90 --r3_sampling=1 --description= --make_searchable=True --translimit=301.0 --iters=1 --r5_peak_search_radius_x=300.0 --r1_iters=5 --r1_cmdiameter_x=100.0 --preprocessing=true --scale=false --iWidth=20 --r2_lowpass_diameter_x=7.36 --dimx=3838 --dimy=3710 --r4_peak_search_radius_x=300.0 --r4_peak_search_radius_y=300.0 --r1_lowpass_apod_x=37.0 --region_x=512 --manual_alignment=False --loglevel=2 --restart_cycle=0 --dose_a=0.245 --lowpass_apod_y=0.05 --lowpass_apod_x=0.05 --kernel_x=3 --kernel_y=3 --clip_low=3.5 --azimuth_max_deviation=2.5 --tilt_clip=true --r4_highpass_apod_x=1910.0 --r4_highpass_apod_y=1910.0 --azimuth=true --r1_mask_width_y=1548 --r1_mask_width_x=1015 --jobtype=protomo --from_multirefine=True node39 Start time: 2019yr06m18d-14hr42m24s Description: Using alignment .tlt file as determined by protomo2multirefine.py.Starting Protomo alignment Beginning Refinements Beginning Refinement Iteration #1, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) lowpass = 14.72 Angstroms Round #1. Parameters in Protomo units: (Note: At binned by 2, pixelsize is 3.67172 Angstroms, Nyquist is 7.34344 Angstroms) window.lowpass.apodization = { 0.198471351351 0.198471351351 } align.translimit = 150.5e0 map.sampling = 2 window.highpass.diameter = { 0.00192236649215 0.00192236649215 } window.highpass.apodization = { 0.00384473298429 0.00384473298429 } window.mask.apodization = { 10 10 } align.mask.width = { 1015 1548 } sampling = 2 align.mask.apodization = { 10 10 } map.body = 550.826873319 map.lowpass.diameter = { 0.498875 0.498875 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 1040 1573 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 1015 1548 } reference.body = 550.826873319 window.lowpass.diameter = { 0.498875 0.498875 } align.correlation.mode = mcf No parameters were switched for Iteration #1 Creating quality assessment statistics... CCMS(shift) = 0.02507 for Iteration #1 of Tilt-Series #10. CCMS(rotations) = 0.24612 for Iteration #1 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #1 of Tilt-Series #10. The scaled sum of CCMS values is #0.01578 for iteration 1 of Tilt-Series #10. Alignment quality for Iteration #1 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Creating Depiction Videos for Iteration #1 in Parallel... Creating Refinement tilt-series video for iteration #1... Generating Refinement reconstruction for iteration #1... The tilt azimuth after iteration #1 deviated from the initial tilt azimuth by 0.894000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #2, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #2 Creating quality assessment statistics... CCMS(shift) = 0.01934 for Iteration #2 of Tilt-Series #10. CCMS(rotations) = 0.21557 for Iteration #2 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #2 of Tilt-Series #10. The scaled sum of CCMS values is #0.01346 for iteration 2 of Tilt-Series #10. Alignment quality for Iteration #2 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Creating Depiction Videos for Iteration #2 in Parallel... Creating Refinement tilt-series video for iteration #2... Generating Refinement reconstruction for iteration #2... The tilt azimuth after iteration #2 deviated from the initial tilt azimuth by 0.826000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #3, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #3 Creating quality assessment statistics... CCMS(shift) = 0.02184 for Iteration #3 of Tilt-Series #10. CCMS(rotations) = 0.29027 for Iteration #3 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #3 of Tilt-Series #10. The scaled sum of CCMS values is #0.01529 for iteration 3 of Tilt-Series #10. Alignment quality for Iteration #3 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Creating Depiction Videos for Iteration #3 in Parallel... Creating Refinement tilt-series video for iteration #3... Generating Refinement reconstruction for iteration #3... The tilt azimuth after iteration #3 deviated from the initial tilt azimuth by 0.601000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #4 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #4, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #4 Creating quality assessment statistics... CCMS(shift) = 0.02099 for Iteration #4 of Tilt-Series #10. CCMS(rotations) = 0.24969 for Iteration #4 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #4 of Tilt-Series #10. The scaled sum of CCMS values is #0.01447 for iteration 4 of Tilt-Series #10. Alignment quality for Iteration #4 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #4 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #4 in Parallel... Creating Refinement tilt-series video for iteration #4... Generating Refinement reconstruction for iteration #4... The tilt azimuth after iteration #4 deviated from the initial tilt azimuth by 0.717000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #5 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #5, Round #1 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #5 Creating quality assessment statistics... CCMS(shift) = 0.01968 for Iteration #5 of Tilt-Series #10. CCMS(rotations) = 0.25173 for Iteration #5 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #5 of Tilt-Series #10. The scaled sum of CCMS values is #0.01406 for iteration 5 of Tilt-Series #10. Alignment quality for Iteration #5 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #5 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #5 in Parallel... Creating Refinement tilt-series video for iteration #5... Generating Refinement reconstruction for iteration #5... The tilt azimuth after iteration #5 deviated from the initial tilt azimuth by 0.580000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #6, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) lowpass = 7.36 angstroms Round #2. Parameters in Protomo units: (Note: At binned by 1, pixelsize is 1.83586 Angstroms, Nyquist is 3.67172 Angstroms) window.lowpass.apodization = { 0.203984444444 0.203984444444 } align.translimit = 301.0e0 map.sampling = 1 window.highpass.diameter = { 0.000961183246073 0.000961183246073 } window.highpass.apodization = { 0.00192236649215 0.00192236649215 } window.mask.apodization = { 10 10 } align.mask.width = { 2056 3121 } sampling = 1 align.mask.apodization = { 10 10 } map.body = 1101.65374664 map.lowpass.diameter = { 0.498875 0.498875 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 2081 3146 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 2056 3121 } reference.body = 1101.65374664 window.lowpass.diameter = { 0.498875 0.498875 } align.correlation.mode = mcf No parameters were switched for Iteration #6 Creating quality assessment statistics... CCMS(shift) = 0.01763 for Iteration #6 of Tilt-Series #10. CCMS(rotations) = 0.24244 for Iteration #6 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #6 of Tilt-Series #10. The scaled sum of CCMS values is #0.01326 for iteration 6 of Tilt-Series #10. Alignment quality for Iteration #6 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #6 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #6 in Parallel... Creating Refinement tilt-series video for iteration #6... Generating Refinement reconstruction for iteration #6... The tilt azimuth after iteration #6 deviated from the initial tilt azimuth by 0.723000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #7 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #7, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #7 Creating quality assessment statistics... CCMS(shift) = 0.01646 for Iteration #7 of Tilt-Series #10. CCMS(rotations) = 0.2111 for Iteration #7 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #7 of Tilt-Series #10. The scaled sum of CCMS values is #0.01245 for iteration 7 of Tilt-Series #10. Alignment quality for Iteration #7 of Tilt-Series #10 (18nov10g): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #7 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #7 in Parallel... Creating Refinement tilt-series video for iteration #7... Generating Refinement reconstruction for iteration #7... The tilt azimuth after iteration #7 deviated from the initial tilt azimuth by 0.713000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #8 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #8, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #8 Creating quality assessment statistics... CCMS(shift) = 0.01819 for Iteration #8 of Tilt-Series #10. CCMS(rotations) = 0.17529 for Iteration #8 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #8 of Tilt-Series #10. The scaled sum of CCMS values is #0.01255 for iteration 8 of Tilt-Series #10. Alignment quality for Iteration #8 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #8 of Tilt-Series #10 (18nov10g): Very StableCreating Depiction Videos for Iteration #8 in Parallel... Creating Refinement tilt-series video for iteration #8... Generating Refinement reconstruction for iteration #8... The tilt azimuth after iteration #8 deviated from the initial tilt azimuth by 0.916000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #9 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #9, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #9 Creating quality assessment statistics... CCMS(shift) = 0.0266 for Iteration #9 of Tilt-Series #10. CCMS(rotations) = 0.32192 for Iteration #9 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #9 of Tilt-Series #10. The scaled sum of CCMS values is #0.0173 for iteration 9 of Tilt-Series #10. Alignment quality for Iteration #9 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #9 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #9 in Parallel... Creating Refinement tilt-series video for iteration #9... Generating Refinement reconstruction for iteration #9... The tilt azimuth after iteration #9 deviated from the initial tilt azimuth by 0.601000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #10 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #10, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #10 Creating quality assessment statistics... CCMS(shift) = 0.01724 for Iteration #10 of Tilt-Series #10. CCMS(rotations) = 0.24429 for Iteration #10 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #10 of Tilt-Series #10. The scaled sum of CCMS values is #0.01315 for iteration 10 of Tilt-Series #10. Alignment quality for Iteration #10 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #10 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #10 in Parallel... Creating Refinement tilt-series video for iteration #10... Generating Refinement reconstruction for iteration #10... The tilt azimuth after iteration #10 deviated from the initial tilt azimuth by 0.736000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #11 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #11, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #11 Creating quality assessment statistics... CCMS(shift) = 0.01812 for Iteration #11 of Tilt-Series #10. CCMS(rotations) = 0.26298 for Iteration #11 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #11 of Tilt-Series #10. The scaled sum of CCMS values is #0.01369 for iteration 11 of Tilt-Series #10. Alignment quality for Iteration #11 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #11 of Tilt-Series #10 (18nov10g): Rock Solid!Creating Depiction Videos for Iteration #11 in Parallel... Creating Refinement tilt-series video for iteration #11... Generating Refinement reconstruction for iteration #11... The tilt azimuth after iteration #11 deviated from the initial tilt azimuth by 0.728000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #12 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #12, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #12 Creating quality assessment statistics... CCMS(shift) = 0.01827 for Iteration #12 of Tilt-Series #10. CCMS(rotations) = 0.16117 for Iteration #12 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #12 of Tilt-Series #10. The scaled sum of CCMS values is #0.01238 for iteration 12 of Tilt-Series #10. Alignment quality for Iteration #12 of Tilt-Series #10 (18nov10g): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #12 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #12 in Parallel... Creating Refinement tilt-series video for iteration #12... Generating Refinement reconstruction for iteration #12... The tilt azimuth after iteration #12 deviated from the initial tilt azimuth by 0.849000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #13 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #13, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #13 Creating quality assessment statistics... CCMS(shift) = 0.01709 for Iteration #13 of Tilt-Series #10. CCMS(rotations) = 0.36617 for Iteration #13 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #13 of Tilt-Series #10. The scaled sum of CCMS values is #0.01472 for iteration 13 of Tilt-Series #10. Alignment quality for Iteration #13 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #13 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #13 in Parallel... Creating Refinement tilt-series video for iteration #13... Generating Refinement reconstruction for iteration #13... The tilt azimuth after iteration #13 deviated from the initial tilt azimuth by 0.457000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #14 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #14, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #14 Creating quality assessment statistics... CCMS(shift) = 0.01835 for Iteration #14 of Tilt-Series #10. CCMS(rotations) = 0.20293 for Iteration #14 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #14 of Tilt-Series #10. The scaled sum of CCMS values is #0.01297 for iteration 14 of Tilt-Series #10. Alignment quality for Iteration #14 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #14 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #14 in Parallel... Creating Refinement tilt-series video for iteration #14... Generating Refinement reconstruction for iteration #14... The tilt azimuth after iteration #14 deviated from the initial tilt azimuth by 0.765000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #15 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #15, Round #2 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #15 Creating quality assessment statistics... CCMS(shift) = 0.01786 for Iteration #15 of Tilt-Series #10. CCMS(rotations) = 0.17342 for Iteration #15 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #15 of Tilt-Series #10. The scaled sum of CCMS values is #0.01241 for iteration 15 of Tilt-Series #10. Alignment quality for Iteration #15 of Tilt-Series #10 (18nov10g): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #15 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #15 in Parallel... Creating Refinement tilt-series video for iteration #15... Generating Refinement reconstruction for iteration #15... The tilt azimuth after iteration #15 deviated from the initial tilt azimuth by 0.861000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. Beginning Refinement Iteration #16, Round #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) lowpass = 3.68 angstroms Round #3. Parameters in Protomo units: (Note: At binned by 1, pixelsize is 1.83586 Angstroms, Nyquist is 3.67172 Angstroms) window.lowpass.apodization = { 0.407968888889 0.407968888889 } align.translimit = 301.0e0 map.sampling = 1 window.highpass.diameter = { 0.000961183246073 0.000961183246073 } window.highpass.apodization = { 0.00192236649215 0.00192236649215 } window.mask.apodization = { 10 10 } align.mask.width = { 2056 3121 } sampling = 1 align.mask.apodization = { 10 10 } map.body = 1101.65374664 map.lowpass.diameter = { 0.99775 0.99775 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 2081 3146 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 2056 3121 } reference.body = 1101.65374664 window.lowpass.diameter = { 0.99775 0.99775 } align.correlation.mode = mcf fit.scale switched from false to true on Iteration #16Creating quality assessment statistics... CCMS(shift) = 0.01629 for Iteration #16 of Tilt-Series #10. CCMS(rotations) = 0.32129 for Iteration #16 of Tilt-Series #10. CCMS(scale) = 0.01243 for Iteration #16 of Tilt-Series #10. The scaled sum of CCMS values is #0.01386 for iteration 16 of Tilt-Series #10. Alignment quality for Iteration #16 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #16 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #16 in Parallel... Creating Refinement tilt-series video for iteration #16... Generating Refinement reconstruction for iteration #16... The tilt azimuth after iteration #16 deviated from the initial tilt azimuth by 0.664000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #17 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #17, Round #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #17 Creating quality assessment statistics... CCMS(shift) = 0.01676 for Iteration #17 of Tilt-Series #10. CCMS(rotations) = 0.22055 for Iteration #17 of Tilt-Series #10. CCMS(scale) = 0.01226 for Iteration #17 of Tilt-Series #10. The scaled sum of CCMS values is #0.01261 for iteration 17 of Tilt-Series #10. Alignment quality for Iteration #17 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #17 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #17 in Parallel... Creating Refinement tilt-series video for iteration #17... Generating Refinement reconstruction for iteration #17... The tilt azimuth after iteration #17 deviated from the initial tilt azimuth by 0.581000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #18 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #18, Round #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #18 Creating quality assessment statistics... CCMS(shift) = 0.01772 for Iteration #18 of Tilt-Series #10. CCMS(rotations) = 0.24319 for Iteration #18 of Tilt-Series #10. CCMS(scale) = 0.01125 for Iteration #18 of Tilt-Series #10. The scaled sum of CCMS values is #0.0129 for iteration 18 of Tilt-Series #10. Alignment quality for Iteration #18 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #18 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #18 in Parallel... Creating Refinement tilt-series video for iteration #18... Generating Refinement reconstruction for iteration #18... The tilt azimuth after iteration #18 deviated from the initial tilt azimuth by 0.485000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #19 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #19, Round #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #19 Creating quality assessment statistics... CCMS(shift) = 0.02165 for Iteration #19 of Tilt-Series #10. CCMS(rotations) = 0.29395 for Iteration #19 of Tilt-Series #10. CCMS(scale) = 0.01186 for Iteration #19 of Tilt-Series #10. The scaled sum of CCMS values is #0.01509 for iteration 19 of Tilt-Series #10. Alignment quality for Iteration #19 of Tilt-Series #10 (18nov10g): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #19 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #19 in Parallel... Creating Refinement tilt-series video for iteration #19... Generating Refinement reconstruction for iteration #19... The tilt azimuth after iteration #19 deviated from the initial tilt azimuth by 0.368000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees. No Round parameters changed for Iteration #20 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) Beginning Refinement Iteration #20, Round #3 of Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400) No parameters were switched for Iteration #20 Creating quality assessment statistics... CCMS(shift) = 0.01577 for Iteration #20 of Tilt-Series #10. CCMS(rotations) = 0.19843 for Iteration #20 of Tilt-Series #10. CCMS(scale) = 0.0081 for Iteration #20 of Tilt-Series #10. The scaled sum of CCMS values is #0.0106 for iteration 20 of Tilt-Series #10. Alignment quality for Iteration #20 of Tilt-Series #10 (18nov10g): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #20 of Tilt-Series #10 (18nov10g): UnstableCreating Depiction Videos for Iteration #20 in Parallel... Creating Refinement tilt-series video for iteration #20... Generating Refinement reconstruction for iteration #20... The tilt azimuth after iteration #20 deviated from the initial tilt azimuth by 0.465000 degrees, which is less than the maximum allowed deviation of 2.500000 degrees.Finished Refinement for Tilt-Series #10 (tiltseries0010_thick1200_thick1200_thick1400)! Did everything blow up and now you're yelling at your computer screen? If so, kindly post your issue on the following Appion-Protomo Google group or email Alex at anoble@nysbc.org explaining the issue and include this log file. https://groups.google.com/forum/#!topic/appion-protomo If everything worked beautifully and you publish, please use the appropriate citations listed on the Appion webpage! You can also print out all citations by typing: protomo2aligner.py --citations End time: 2019yr06m19d-22hr54m55s