DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0015/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0015/defocus_estimation/series0015_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0015/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -50.6 46000.0 35000.0 45000.0 1 -48.4 45000.0 34500.0 34000.0 2 -46.2 33000.0 47000.0 42500.0 3 -44.0 41000.0 41500.0 38000.0 4 -41.8 45000.0 43500.0 40000.0 5 -39.6 44500.0 45000.0 48000.0 6 -37.4 44500.0 47500.0 43000.0 7 -35.2 46500.0 32000.0 41500.0 8 -33.0 44000.0 38500.0 41000.0 9 -30.8 40500.0 41500.0 41500.0 10 -28.6 39500.0 41500.0 41500.0 11 -26.4 41500.0 39500.0 39000.0 12 -24.2 40500.0 41500.0 40000.0 13 -22.0 39500.0 42000.0 42000.0 14 -19.8 41000.0 41500.0 41500.0 15 -17.6 38500.0 41500.0 42000.0 16 -15.4 43500.0 45000.0 40500.0 17 -13.2 42500.0 38500.0 42000.0 18 -11.0 41500.0 41000.0 42000.0 19 -8.8 42000.0 43000.0 44500.0 20 -6.6 41500.0 41500.0 42000.0 21 -4.4 42500.0 44000.0 40500.0 22 -2.2 32000.0 42000.0 43500.0 23 -0.0 43000.0 40500.0 44000.0 24 0.0 44500.0 44000.0 42500.0 25 2.2 42000.0 42000.0 43500.0 26 4.4 41500.0 45500.0 43500.0 27 6.6 41500.0 44500.0 44000.0 28 8.8 42000.0 44000.0 43500.0 29 11.0 44000.0 41500.0 42000.0 30 13.2 41500.0 43000.0 45500.0 31 15.4 42000.0 42000.0 44500.0 32 17.6 41500.0 42500.0 41000.0 33 19.8 41500.0 36500.0 43500.0 34 22.0 40500.0 43500.0 45500.0 35 24.2 43000.0 41500.0 41500.0 36 26.4 41500.0 44500.0 44000.0 37 28.6 37500.0 38500.0 42500.0 38 30.8 38500.0 42000.0 40500.0 39 33.0 38500.0 41500.0 39500.0 40 35.2 40000.0 38500.0 43000.0 41 37.4 37500.0 43500.0 42500.0 42 39.6 37500.0 39000.0 40000.0 43 41.8 37000.0 38000.0 35000.0 44 44.0 36000.0 44500.0 47500.0 45 46.2 36500.0 39500.0 36000.0 46 48.4 33500.0 47500.0 41000.0 47 50.6 36000.0 37000.0 41500.0 48 52.8 32000.0 32000.0 32000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 108.826u 0.991s 1:50.08 99.7% 0+0k 0+0io 0pf+0w