DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0017/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0017/defocus_estimation/series0017_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0017/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -41.8 35000.0 38500.0 47000.0 1 -39.6 33000.0 32000.0 33500.0 2 -37.4 34000.0 32000.0 32000.0 3 -35.2 33000.0 32500.0 45500.0 4 -33.0 33000.0 33500.0 40000.0 5 -30.8 32000.0 32000.0 43500.0 6 -28.6 32500.0 34000.0 40000.0 7 -26.4 32500.0 32000.0 32000.0 8 -24.2 32000.0 34000.0 46000.0 9 -22.0 32500.0 32000.0 40000.0 10 -19.8 32500.0 32000.0 32000.0 11 -17.6 32500.0 32000.0 35500.0 12 -15.4 32000.0 32000.0 34000.0 13 -13.2 33000.0 32500.0 47500.0 14 -11.0 32000.0 33000.0 34500.0 15 -8.8 33000.0 33000.0 48000.0 16 -6.6 32500.0 32000.0 32000.0 17 -4.4 33000.0 33000.0 32500.0 18 -2.2 33500.0 34500.0 32000.0 19 -0.0 36000.0 34500.0 32000.0 20 0.0 33000.0 33500.0 32500.0 21 2.2 34500.0 33500.0 33500.0 22 4.4 33000.0 33000.0 33000.0 23 6.6 33000.0 32500.0 32000.0 24 8.8 32500.0 33500.0 32500.0 25 11.0 32500.0 32500.0 32500.0 26 13.2 33000.0 32500.0 34000.0 27 15.4 32000.0 32000.0 32500.0 28 17.6 33000.0 33000.0 33000.0 29 19.8 33500.0 32000.0 33500.0 30 22.0 32000.0 33000.0 34500.0 31 24.2 32000.0 32000.0 32500.0 32 26.4 32500.0 32500.0 34500.0 33 28.6 32000.0 32000.0 32500.0 34 30.8 32000.0 33000.0 34000.0 35 33.0 37000.0 33000.0 35000.0 36 35.2 32000.0 32000.0 36000.0 37 37.4 32000.0 32000.0 34000.0 38 39.6 38500.0 32500.0 34500.0 39 41.8 47500.0 34500.0 34000.0 40 44.0 36500.0 32000.0 32000.0 41 46.2 46000.0 36500.0 35000.0 42 48.4 46000.0 33000.0 35000.0 43 50.6 35500.0 36500.0 35000.0 44 52.8 46000.0 32000.0 34500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 95.774u 1.141s 1:43.44 93.6% 0+0k 0+0io 0pf+0w