DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0022/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0022/defocus_estimation/series0022_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0022/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -39.6 0.0 35000.0 32000.0 1 -37.4 3848519680.0 32000.0 40000.0 2 -35.2 0.0 41500.0 39000.0 3 -33.0 3920701440.0 34500.0 34000.0 4 -30.8 0.0 36500.0 33500.0 5 -28.6 3992883200.0 32500.0 43500.0 6 -26.4 0.0 32000.0 39000.0 7 -24.2 4065064960.0 32000.0 32500.0 8 -22.0 0.0 40500.0 35000.0 9 -19.8 4137246720.0 32500.0 39500.0 10 -17.6 0.0 32000.0 34000.0 11 -15.4 4209428480.0 33000.0 32000.0 12 -13.2 0.0 33000.0 33500.0 13 -11.0 4281610240.0 33500.0 34500.0 14 -8.8 0.0 32000.0 40000.0 15 -6.6 4412616704.0 32000.0 33000.0 16 -4.4 0.0 33000.0 38500.0 17 -2.2 4556980224.0 43500.0 36500.0 18 -0.0 0.0 32500.0 33500.0 19 0.0 32000.0 32000.0 32000.0 20 2.2 32500.0 32000.0 32000.0 21 4.4 33000.0 33000.0 34000.0 22 6.6 32000.0 32500.0 32000.0 23 8.8 32000.0 32000.0 35000.0 24 11.0 32000.0 32000.0 32000.0 25 13.2 40000.0 32000.0 32500.0 26 15.4 32000.0 33500.0 34500.0 27 17.6 32000.0 32500.0 32000.0 28 19.8 32000.0 38000.0 35500.0 29 22.0 32000.0 34000.0 34500.0 30 24.2 33500.0 39000.0 34000.0 31 26.4 36000.0 32000.0 32000.0 32 28.6 32000.0 32000.0 42000.0 33 30.8 36500.0 38000.0 32000.0 34 33.0 32000.0 47000.0 35000.0 35 35.2 40500.0 38000.0 33000.0 36 37.4 48000.0 33500.0 36000.0 37 39.6 44500.0 33000.0 32000.0 38 41.8 36000.0 38500.0 40000.0 39 44.0 34500.0 32000.0 42500.0 40 46.2 35500.0 35000.0 39500.0 41 48.4 48000.0 34500.0 48000.0 42 50.6 45000.0 48000.0 38500.0 43 52.8 38500.0 41500.0 44000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 90.715u 0.981s 1:37.78 93.7% 0+0k 0+0io 0pf+0w